A Survey of
            <i>Helicobacter pylori</i>
            Antibiotic-Resistant Genotypes and Strain Lineages by Whole-Genome Sequencing in China

Zhou, Yan; Zhong, Zishao; Hu, Shengjuan; Wang, Jing; Deng, Yanhong; Li, Ximei; Chen, Xianmei; Li, Xue; Tang, Yuanyuan; Li, Xiaofei; Hao, Qian; Li, Jun; Sang, Tian; Yang, Bo; Bai, Feihu

doi:10.1128/aac.02188-21

Cited by 18 publications

(29 citation statements)

References 80 publications

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“…This result is in agreement with the previous studies. 25,51,52 Therefore, resistance to clarithromycin and levofloxacin can be detected based on the genotype. Metronidazole resistance is frequently associated with loss of rdxA function.…”

Section: Concordance Analysis Of Phenotypic and Genotypic Resistancementioning

confidence: 99%

Characteristics of phenotypic antibiotic resistance of Helicobacter pylori and its correlation with genotypic antibiotic resistance: A retrospective study in Ningxia

Zhou

Deng

et al. 2023

Helicobacter

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Background Geographic differences exist in the antibiotic resistance patterns of Helicobacter pylori. Personalized treatment regimens based on local or individual resistance data are essential. We evaluated the current status of H. pylori resistance in Ningxia, analyzed resistance‐related factors, and assessed the concordance of phenotypic and genotypic resistance. Methods Strains were isolated from the gastric mucosa of patients infected with H. pylori in Ningxia and relevant clinical information was collected. Phenotypic antibiotic susceptibility assays (Kirby–Bauer disk diffusion) and antibiotic resistance gene detection (Sanger sequencing) were performed. Results We isolated 1955 H. pylori strains. The resistance rates of H. pylori to amoxicillin, levofloxacin, clarithromycin, and metronidazole were 0.9%, 42.4%, 40.4%, and 94.2%, respectively. Only five tetracycline‐resistant and one furazolidone‐resistant strain were identified. Overall, 3.3% of the strains were sensitive to all six antibiotics. Multidrug‐resistant strains accounted for 22.9%, of which less than 20% were from Wuzhong. Strains isolated from women and patients with nonulcerative disease had higher rates of resistance to levofloxacin and clarithromycin. Higher rates of resistance to metronidazole, levofloxacin, and clarithromycin were observed in the older age group than in the younger age group. The kappa coefficients of phenotypic resistance and genotypic resistance for levofloxacin and clarithromycin were 0.830 and 0.809, respectively, whereas the remaining antibiotics showed poor agreement. Conclusion H. pylori antibiotic resistance is severe in Ningxia. Therefore, furazolidone, amoxicillin, and tetracycline are better choices for the empirical therapy of H. pylori infection in this region. Host sex, age, and the presence of ulcerative diseases may affect antibiotic resistance of the bacteria. Personalized therapy based on genetic testing for levofloxacin and clarithromycin resistance may be a future direction for the eradication therapy of H. pylori infection in Ningxia.

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Section: Concordance Analysis Of Phenotypic and Genotypic Resistancementioning

confidence: 99%

Characteristics of phenotypic antibiotic resistance of Helicobacter pylori and its correlation with genotypic antibiotic resistance: A retrospective study in Ningxia

Zhou

Deng

et al. 2023

Helicobacter

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“…Currently, clarithromycin, amoxicillin and metronidazole are recommended for the treatment. However, mutations in H. pylori genes have led to the treatment failure ( 156 ).…”

Section: Methicillin-resistant Staphylococcus Aureus ...mentioning

confidence: 99%

Cell membrane-coated nanoparticles: An emerging antibacterial platform for pathogens of food animals

Altaf

Alkheraije

2023

Front. Vet. Sci.

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Bacterial pathogens of animals impact food production and human health globally. Food animals act as the major host reservoirs for pathogenic bacteria and thus are highly prone to suffer from several endemic infections such as pneumonia, sepsis, mastitis, and diarrhea, imposing a major health and economical loss. Moreover, the consumption of food products of infected animals is the main route by which human beings are exposed to zoonotic bacteria. Thus, there is excessive and undue administration of antibiotics to fight these virulent causative agents of food-borne illness, leading to emergence of resistant strains. Thus, highprevalence antibiotic-resistant resistant food-borne bacterial infections motivated the researchers to discover new alternative therapeutic strategies to eradicate resistant bacterial strains. One of the successful therapeutic approach for the treatment of animal infections, is the application of cell membrane-coated nanoparticles. Cell membranes of several different types of cells including platelets, red blood cells, neutrophils, cancer cells, and bacteria are being wrapped over the nanoparticles to prepare biocompatible nanoformulations. This diversity of cell membrane selection and together with the possibility of combining with an extensive range of nanoparticles, has opened a new opportunistic window for the development of more potentially effective, safe, and immune evading nanoformulations, as compared to conventionally used bare nanoparticle. This article will elaborately discuss the discovery and development of novel bioinspired cell membrane-coated nanoformulations against several pathogenic bacteria of food animals such as Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, Salmonella enteritidis, Campylobacter jejuni, Helicobacter pylori, and Group A Streptococcus and Group B Streptococcus.

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“…The treatment strategy should meet the needs of personalized and precise diagnosis as well as complying with the standardized treatment regimen on the consensus. The China Center for H. pylori Molecular Medicine (CCHpMM) (advocated by the late Professor Xiao Shudong, a gastroenterologist) and its nationwide branches have conducted a series of H. pylori culture and antibiotic susceptibility tests, antibiotic resistance gene mutation detection, immunohistochemistry for coccoid transformation (for those who have failed in eradication), H. pylori whole genome sequencing, host CYP2C19 gene polymorphism, and other H. pylori related tests, providing a solid foundation for basic and clinical research and application of H. pylori in China 31 . CCHpMM and its branches devote to create an H. pylori epidemiological map, antibiotic resistance map, and molecular epidemiological map of antibiotic resistance genes in China, which will provide an important reference for the rational selection of H. pylori eradication treatment regimens in different regions 8 .…”

Section: Pre‐treatment Strategymentioning

confidence: 99%

Achieving Helicobacter pylori eradication in the primary treatment requires a deep integration of personalization and standardization

Zhong

Zhan²,

Xu³

et al. 2022

Helicobacter

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A Survey of Helicobacter pylori Antibiotic-Resistant Genotypes and Strain Lineages by Whole-Genome Sequencing in China

Cited by 18 publications

References 80 publications

Characteristics of phenotypic antibiotic resistance of Helicobacter pylori and its correlation with genotypic antibiotic resistance: A retrospective study in Ningxia

Characteristics of phenotypic antibiotic resistance of Helicobacter pylori and its correlation with genotypic antibiotic resistance: A retrospective study in Ningxia

Cell membrane-coated nanoparticles: An emerging antibacterial platform for pathogens of food animals

Achieving Helicobacter pylori eradication in the primary treatment requires a deep integration of personalization and standardization

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