A survey of introns in three genes of rotifers

Welch, David B. Mark; Meselson, Matthew

doi:10.1007/978-94-010-0756-6_43

Cited by 4 publications

(3 citation statements)

References 15 publications

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“…The hsp82 and histone gene cluster contigs in both species are gene rich with exons constituting approximately 50% of the sequenced 800 kb. The lack of transposable genetic elements and the abundance of small introns in these regions are consistent with findings reported for P. roseola hsp82 regions and other gene-rich regions of bdelloid genomes Meselson 2000, 2005;Mark Welch and Meselson 2001;Mark Welch et al 2008;Gladyshev, Meselson, and Arkhipova 2008). There are introns even in the canonical histone genes contrary to the general lack of introns in histone genes of other species.…”

Section: Introns and Transposable Elementssupporting

confidence: 87%

Degenerate Tetraploidy Was Established Before Bdelloid Rotifer Families Diverged

Hur

Doninck

Mandigo

et al. 2008

Molecular Biology and Evolution

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Rotifers of Class Bdelloidea are abundant freshwater invertebrates known for their remarkable ability to survive desiccation and their lack of males and meiosis. Sequencing and annotation of approximately 50-kb regions containing the four hsp82 heat shock genes of the bdelloid Philodina roseola, each located on a separate chromosome, have suggested that its genome is that of a degenerate tetraploid. In order to determine whether a similar structure exists in a bdelloid distantly related to P. roseola and if degenerate tetraploidy was established before the two species separated, we sequenced regions containing the hsp82 genes of a bdelloid belonging to a different family, Adineta vaga, and the histone gene clusters of P. roseola and A. vaga. Our findings are entirely consistent with degenerate tetraploidy and show that it was established before the two bdelloid families diverged and therefore probably before the bdelloid radiation.

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Section: Introns and Transposable Elementssupporting

confidence: 87%

Degenerate Tetraploidy Was Established Before Bdelloid Rotifer Families Diverged

Hur

Doninck

Mandigo

et al. 2008

Molecular Biology and Evolution

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“…An important next step is to understand the genetic basis of resting egg formation. Various studies on single gene function have been published in recent years (Wheelock et al, 1999;Kaneko et al, 2002Kaneko et al, , 2005, and increasing molecular information has been available on bdelloid rotifers (Mark Welch & Meselson, 2001;Mark Welch & Mark Welch 2005;Pouchkina-Stantcheva et al, 2007) and monogonont brachionid rotifers (Suga et al, 2007a(Suga et al, , 2007b(Suga et al, , 2008; however, there is almost no information on gene regulation in the brachionid rotifer life cycle.…”

Section: Introductionmentioning

confidence: 99%

Isolation of mixis-related genes from the rotifer Brachionus plicatilis using subtractive hybridization

et al. 2010

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The monogonont rotifer Brachionus plicatilis produces resting eggs through sexual reproduction (mixis), which is affected by external and internal factors. We collected resting eggs from rotifers cultured at 15 and 25ºC and hatched them with and without 14-day dormancy in the dark. Stem females hatched from both conditions were further cultured at 15, 20 and 25ºC. We obtained two phenotypes, one with high mixis (more than 50%), which was hatched from resting eggs formed at 15ºC without dormancy, and another in which sexual reproduction occurred at 25ºC and resting eggs were formed at 15ºC with a 14-day dormancy. In the latter phenotype, mictic females appeared at 15ºC, but not at 25ºC. Using subtractive hybridization, we isolated one gene from the latter phenotype of females that had no significant similarity to known genes in BLAST searches.We propose that this gene is unique to rotifer mictic reproduction. Ongoing characterization of this gene attempts understand its role in mixis.3

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“…Furthermore, the genomes of each of seven sexually reproducing rotifers have been found to contain either two nearly identical sequences or only a single sequence of the stress-inducible gene HSP82 for a so-called heat-shock protein, while the four bdelloid genomes examined contain two or more divergent copies of this gene in comparison (Mark Welch & Meselson 2000). The asexual bdelloids also have more copies of other genes (Mark Welch & Meselson 1998, 2001a; these copies are more divergent (Mark Welch & Meselson 2000, 2001a and contain more introns (Mark Welch & Meselson 2000, 2001a than those of the monogonont.…”

Section: Some Evidence On Ancient Asexual Scandalsmentioning

confidence: 99%

Strategies of Sexual Reproduction in Aphids

Joachim¹

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II AcknowledgementsMy visit at the University of East Anglia in Norwich was funded by the Deutscher Akademischer Austausch Dienst. I thank Prof. Anthony F. G. Dixon for excellent support and supervision in Norwich and Prof. Stefan Vidal for the same in Göttingen.Further thanks go to: Prof. Matthias Schaefer for yielding the theme to me; Thomas Thieme for a tip on preparing the sperm storage organ of female aphids and pictures of Euceraphis betulae and Uroleucon cichorii that have been presented during a talk at Rennes (France); Geoffrey A. Parker for discussing his old and classic publications on yellow dung flies with me via e-mail; Sebastian Domhof for precious statistical advice as well as Lars Reimer for helpful chats on statistics; Angelika Metje for tending thistles; Fabien Halkett for a tip at

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A survey of introns in three genes of rotifers

Cited by 4 publications

References 15 publications

Degenerate Tetraploidy Was Established Before Bdelloid Rotifer Families Diverged

Degenerate Tetraploidy Was Established Before Bdelloid Rotifer Families Diverged

Isolation of mixis-related genes from the rotifer Brachionus plicatilis using subtractive hybridization

Strategies of Sexual Reproduction in Aphids

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