A Survey of Pyridoxal 5′-Phosphate-Dependent Proteins in the Gram-Positive Model Bacterium Bacillus subtilis

Richts, Björn; Rosenberg, Jonathan B.; Commichau, Fabian M.

doi:10.3389/fmolb.2019.00032

Cited by 43 publications

(42 citation statements)

References 221 publications

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“…Pyridoxal-5-P (vitamin B 6 ) is a cofactor required by a range of enzymes involved in amino acid biosynthesis and catabolism, iron, cell wall component and carbon metabolism, and biosynthesis of other cofactors (For a full list refer to [120]). Biosynthesis of pyridoxal-5-P in E. coli utilizes 1-deoxy-D-xylulose-5-P and 3-amino-2-oxopropyl phosphate as substrates, and is catalysed via PdxA/PdxJ, then PdxH ( Figure 6H) [121].…”

Section: Pyridoxal-5p Biosyntheismentioning

confidence: 99%

Current knowledge and recent advances in understanding metabolism of the model cyanobacteriumSynechocystissp. PCC 6803

2020

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Cyanobacteria are key organisms in the global ecosystem, useful models for studying metabolic and physiological processes conserved in photosynthetic organisms, and potential renewable platforms for production of chemicals. Characterizing cyanobacterial metabolism and physiology is key to understanding their role in the environment and unlocking their potential for biotechnology applications. Many aspects of cyanobacterial biology differ from heterotrophic bacteria. For example, most cyanobacteria incorporate a series of internal thylakoid membranes where both oxygenic photosynthesis and respiration occur, while CO2 fixation takes place in specialized compartments termed carboxysomes. In this review, we provide a comprehensive summary of our knowledge on cyanobacterial physiology and the pathways in Synechocystis sp. PCC 6803 (Synechocystis) involved in biosynthesis of sugar-based metabolites, amino acids, nucleotides, lipids, cofactors, vitamins, isoprenoids, pigments and cell wall components, in addition to the proteins involved in metabolite transport. While some pathways are conserved between model cyanobacteria, such as Synechocystis, and model heterotrophic bacteria like Escherichia coli, many enzymes and/or pathways involved in the biosynthesis of key metabolites in cyanobacteria have not been completely characterized. These include pathways required for biosynthesis of chorismate and membrane lipids, nucleotides, several amino acids, vitamins and cofactors, and isoprenoids such as plastoquinone, carotenoids, and tocopherols. Moreover, our understanding of photorespiration, lipopolysaccharide assembly and transport, and degradation of lipids, sucrose, most vitamins and amino acids, and haem, is incomplete. We discuss tools that may aid our understanding of cyanobacterial metabolism, notably CyanoSource, a barcoded library of targeted Synechocystis mutants, which will significantly accelerate characterization of individual proteins.

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Section: Pyridoxal-5p Biosyntheismentioning

confidence: 99%

Current knowledge and recent advances in understanding metabolism of the model cyanobacteriumSynechocystissp. PCC 6803

2020

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“…Other thiamin dependent enzymes are transketolase, αketoacid decarboxylase, α-ketoacid dehydrogenase and acetolactate synthase (9). A very similar scenario was observed for pyridoxal 5'-phosphate (vitamin B6) in the model Gram-positive organism Bacillus subtilis (10).…”

Section: Introductionmentioning

confidence: 58%

Essential Metabolic Routes as a Way to ESKAPE from Antibiotic Resistance

Barra

Dantas

Morão

et al. 2019

Preprint

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The antibiotic resistance is a worldwide concern and that requires a concerted action from physicians, patients, governmental agencies and academia to prevent infections and the spread of resistance, to track resistant bacteria, to improve the use of current antibiotics and to develop new antibiotics. Despite the efforts spent so far, the current antibiotics in the market are restricted to only five general targets/pathways highlighting the need for basic research focusing on the discovery and evaluation of new potential targets. Here we interrogate two biosynthetic pathways as potentially druggable pathways in bacteria. The biosynthesis pathway for thiamine (vitamin B1), absent in humans, but found in many bacteria, including pathogenic organism in the group of the ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumanii, Pseudomonas aeruginosa and Enterobacter species) and the biosynthesis pathway for pyridoxal 5'phosphate and its vitamers (vitamin B6), found in S. aureus. Using current genomic data, we discuss the possibilities of inhibition of enzymes in the pathway and review the current state of the art in the scientific literature.

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“…Other thiamin-dependent enzymes are transketolase, α-ketoacid decarboxylase, α-ketoacid dehydrogenase, and acetolactate synthase (9). A very similar scenario was observed for pyridoxal 5 ′ -phosphate (vitamin B6) in the model Gram-positive organism Bacillus subtilis (10).…”

Section: Introductionmentioning

confidence: 60%

Essential Metabolic Routes as a Way to ESKAPE From Antibiotic Resistance

Barra

Dantas

Morão

et al. 2020

Front. Public Health

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Antibiotic resistance is a worldwide concern that requires a concerted action from physicians, patients, governmental agencies, and academia to prevent infections and the spread of resistance, track resistant bacteria, improve the use of current antibiotics, and develop new antibiotics. Despite the efforts spent so far, the current antibiotics in the market are restricted to only five general targets/pathways highlighting the need for basic research focusing on the discovery and evaluation of new potential targets. Here we interrogate two biosynthetic pathways as potentially druggable pathways in bacteria. The biosynthesis pathway for thiamine (vitamin B1), absent in humans, but found in many bacteria, including organisms in the group of the ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumanii, Pseudomonas aeruginosa, and Enterobacter sp.) and the biosynthesis pathway for pyridoxal 5 ′ -phosphate and its vitamers (vitamin B6), found in S. aureus.Using current genomic data, we discuss the possibilities of inhibition of enzymes in the pathway and review the current state of the art in the scientific literature.

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A Survey of Pyridoxal 5′-Phosphate-Dependent Proteins in the Gram-Positive Model Bacterium Bacillus subtilis

Cited by 43 publications

References 221 publications

Current knowledge and recent advances in understanding metabolism of the model cyanobacteriumSynechocystissp. PCC 6803

Current knowledge and recent advances in understanding metabolism of the model cyanobacteriumSynechocystissp. PCC 6803

Essential Metabolic Routes as a Way to ESKAPE from Antibiotic Resistance

Essential Metabolic Routes as a Way to ESKAPE From Antibiotic Resistance

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