Three new Otacilia species were collected from Jiangxi Provinces, China during a survey of the spider fauna of the region: Otacilia anfu Liu, sp. nov. (♂♀), O. guanshan Liu, sp. nov. (♂♀), and O. mingyueshan Liu, sp. nov. (♂♀). All species are described and illustrated with photographs and SEM micrographs, and their distribution is also mapped.