A survey of the pyrabactin resistance-like abscisic acid receptor gene family in poplar

Yu, Jingling; Li, Hejuan; Peng, Yi; Yang, Lun; Zhao, Fugeng; Luan, Sheng

doi:10.1080/15592324.2017.1356966

Cited by 5 publications

(7 citation statements)

References 12 publications

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“…The expression of distinct RCARs decreased under drought conditions (Cohen et al ., ) and recovered upon re‐watering (Bizet et al ., ). Overexpression of RCAR1 in Arabidopsis as well as in poplar resulted in more drought tolerant plants (Ma et al ., ; Yu et al ., , ,b), while only multiple RCAR knock‐out mutants exhibited enhanced stress sensitivity (Park et al ., ; González‐Guzmán et al ., ). In our study, we used poplar lines overexpressing the ABA receptor RCAR1/PYL9 (Singh et al ., ).…”

Section: Discussionmentioning

confidence: 99%

“…For example, the poplar RCAR family (also denoted as PYR/PYL/RCAR or PYRL) has 14 members (Papacek et al, 2017). Overexpression of PYRL1 and PYRL5 in poplar (denoted after Yu et al, 2017b) confers drought tolerance (Yu et al, 2017a). In Arabidopsis, overexpression of AtRCAR1/PYL9 results in higher survival after drought than overexpression of other members of the RCAR gene family (Zhao et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Abscisic acid signalling mediates biomass trade‐off and allocation in poplar

Wildhagen

Tylewicz

et al. 2019

New Phytologist

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Summary Abscisic acid (ABA) is a well known stress hormone regulating drought adaptation of plants. Here, we hypothesised that genetic engineering of genes involved in ABA stress signalling and photoperiodic regulation affected drought resistance by trade‐off with biomass production in perennial poplar trees. We grew Populus tremula × tremuloides wild‐type (T89) and various transgenic lines (two transformation events of 35S::abi1‐1, 35S::RCAR, RCAR:RNAi, 35S::ABI3, 35S::AREB3, 35S::FDL1, FDL1:RNAi, 35S::FDL2 and FDL2:RNAi) outdoors and exposed them to drought in the second growth period. After the winter, the surviving lines showed a huge variation in stomatal conductance, leaf size, whole‐plant leaf area, tree height, stem diameter, and biomass. Whole‐plant leaf area was a strong predictor for woody biomass production. The 35S::AREB3 lines were compromised in biomass production under well irrigated conditions compared with wild‐type poplars but were resilient to drought. ABA signalling regulated FDL1 and FDL2 expression under stress. Poplar lines overexpressing FDL1 or FDL2 were drought‐sensitive; they shed leaves and lost root biomass, whereas the FDL RNAi lines showed higher biomass allocation to roots under drought. These results assign a new function in drought acclimation to FDL genes aside from photoperiodic regulation. Our results imply a critical role for ABA‐mediated processes in balancing biomass production and climate adaptation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Abscisic acid signalling mediates biomass trade‐off and allocation in poplar

Wildhagen

Tylewicz

et al. 2019

New Phytologist

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“…The phytohormone abscisic acid (ABA) signaling pathway is an important pathway for higher plants to regulate biotic and abiotic stress, and plays an important role in regulating plant tolerance and growth and development [ 13 ]. This pathway mainly involves the ABA receptor protein (PYR/PYL/RCAR), PP2Cs, and SNF1-associated protein kinase 2 (SnRK2s) [ 14 , 15 , 16 ]. ABA can reportedly interact with the receptor protein PYR/PYL/RCAR and regulate SnRK2-type protein kinase activity through negative regulation, which plays an important role in regulating PP2C in connection with transduction pathways [ 12 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and Abiotic Stress Response Analysis of PP2C Gene Family in Woodland and Pineapple Strawberries

Guo

Liu

et al. 2023

IJMS

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Protein phosphatase 2C (PP2C) is a negative regulator of serine/threonine residue protein phosphatase and plays an important role in abscisic acid (ABA) and abiotic-stress-mediated signaling pathways in plants. The genome complexity of woodland strawberry and pineapple strawberry is different due to the difference in chromosome ploidy. This study conducted a genome-wide investigation of the FvPP2C (Fragaria vesca) and FaPP2C (Fragaria ananassa) gene family. Fifty-six FvPP2C genes and 228 FaPP2C genes were identified from the woodland strawberry and pineapple strawberry genomes, respectively. FvPP2Cs were distributed on seven chromosomes, and FaPP2Cs were distributed on 28 chromosomes. The size of the FaPP2C gene family was significantly different from that of the FvPP2C gene family, but both FaPP2Cs and FvPP2Cs were localized in the nucleus, cytoplasm, and chloroplast. Phylogenetic analysis revealed that 56 FvPP2Cs and 228 FaPP2Cs could be divided into 11 subfamilies. Collinearity analysis showed that both FvPP2Cs and FaPP2Cs had fragment duplication, and the whole genome duplication was the main cause of PP2C gene abundance in pineapple strawberry. FvPP2Cs mainly underwent purification selection, and there were both purification selection and positive selection effects in the evolution of FaPP2Cs. Cis-acting element analysis found that the PP2C family genes of woodland and pineapple strawberries mainly contained light responsive elements, hormone responsive elements, defense and stress responsive elements, and growth and development-related elements. The results of quantitative real-time PCR (qRT-PCR) showed that the FvPP2C genes showed different expression patterns under ABA, salt, and drought treatment. The expression level of FvPP2C18 was upregulated after stress treatment, which may play a positive regulatory role in ABA signaling and abiotic stress response mechanisms. This study lays a foundation for further investigation on the function of the PP2C gene family.

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“…Circadian components and ABA-signalling pathways are largely conserved between Arabidopsis and Populus ( Ibáñez et al, 2010 ; Kozarewa et al, 2010 ; Cai et al, 2017 ; Yu et al, 2017 ; Tylewicz et al, 2018 ; Rigoulot et al, 2019 ). We hypothesised that ZTL’s roles in regulating the circadian clock and physiological responses to drought and ABA would also be conserved.…”

Section: Resultsmentioning

confidence: 99%

ZEITLUPE Promotes ABA-Induced Stomatal Closure in Arabidopsis and Populus

et al. 2022

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Plants balance water availability with gas exchange and photosynthesis by controlling stomatal aperture. This control is regulated in part by the circadian clock, but it remains unclear how signalling pathways of daily rhythms are integrated into stress responses. The serine/threonine protein kinase OPEN STOMATA 1 (OST1) contributes to the regulation of stomatal closure via activation of S-type anion channels. OST1 also mediates gene regulation in response to ABA/drought stress. We show that ZEITLUPE (ZTL), a blue light photoreceptor and clock component, also regulates ABA-induced stomatal closure in Arabidopsis thaliana, establishing a link between clock and ABA-signalling pathways. ZTL sustains expression of OST1 and ABA-signalling genes. Stomatal closure in response to ABA is reduced in ztl mutants, which maintain wider stomatal apertures and show higher rates of gas exchange and water loss than wild-type plants. Detached rosette leaf assays revealed a stronger water loss phenotype in ztl-3, ost1-3 double mutants, indicating that ZTL and OST1 contributed synergistically to the control of stomatal aperture. Experimental studies of Populus sp., revealed that ZTL regulated the circadian clock and stomata, indicating ZTL function was similar in these trees and Arabidopsis. PSEUDO-RESPONSE REGULATOR 5 (PRR5), a known target of ZTL, affects ABA-induced responses, including stomatal regulation. Like ZTL, PRR5 interacted physically with OST1 and contributed to the integration of ABA responses with circadian clock signalling. This suggests a novel mechanism whereby the PRR proteins—which are expressed from dawn to dusk—interact with OST1 to mediate ABA-dependent plant responses to reduce water loss in time of stress.

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A survey of the pyrabactin resistance-like abscisic acid receptor gene family in poplar

Cited by 5 publications

References 12 publications

Abscisic acid signalling mediates biomass trade‐off and allocation in poplar

Abscisic acid signalling mediates biomass trade‐off and allocation in poplar

Genome-Wide Identification and Abiotic Stress Response Analysis of PP2C Gene Family in Woodland and Pineapple Strawberries

ZEITLUPE Promotes ABA-Induced Stomatal Closure in Arabidopsis and Populus

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