A survey of True flies (Insecta: Diptera) by DNA Barcoding of Malaise Trap Collection in Bangladesh

Abstract

True flies (Insecta: Diptera) are widely distributed and the key components in most ecosystems. The objective of this study was to identify the true flies' diversity through DNA barcoding technique (658 bp sequence from the 5′-end of cytochromeoxidase I) in Bangladesh. Specimens were collected by a Malaise trap at Chittagong University Campus between April 2014 and March 2015. In this study, we examined 36476 sequences of 38267 true flies, and resulted 105 species, 109 genera, 54 subfamilies and 59 families. Among them 79 species, 69 genera, 12 subfamilies and 23 families are new country records. All the specimen records with the Barcode Index Numbers (BINs) (the species proxies), are available on the Barcode of Life Data System (BOLD).

DNA barcoding of the horsefly fauna (Diptera, Tabanidae) of Croatia with notes on the morphology and taxonomy of selected species from Chrysopsinae and Tabaninae

“…Distinguishing these morphological features can be difficult, leading to misidentifications. Therefore, many recent studies utilize DNA barcoding to verify/supplement their findings ( Banerjee et al 2015 ; Nitiyamatawat et al 2017 ; Changbunjong et al 2018 ; Mugasa et al 2018 ; Morinière et al 2019 ; Changbunjong et al 2020 ; Mazumdar et al 2021 ). DNA barcoding is established as a universal tool in biodiversity research, ensuring rapid and accurate species identification independent of the developmental stage ( Hebert et al 2003 ).…”