A Survey of Viral Diseases of Proso Millet (Panicum miliaceum L.) and Sorghum (Sorghum bicolor L.) in South Korea

Abstract

Throughout year 2015 to 2016, 101 proso millet and 200 sorghum samples were collected from five provinces in South Korea. The samples were subjected to paired-end RNA sequencing and further analyzed by RT-PCR. The results indicated that Rice black-streaked dwarf virus (RBSDV) was detected from sorghum collected in Gyeongsang province. The other four viruses, including RBSDV, Rice stripe virus (RSV), Barley virus G (BVG), and Cereal yellow dwarf virus (CYDV), were detected from proso millet. Among four viruses, both RSV and RBSDV were identified high frequency from proso millet collected from Gyeongsang province. Otherwise, BVG was nearly equally identified from five provinces, suggesting that the virus was supposedly widespread nationwide. RBSDV was first identified from both proso millet and sorghum in South Korea. The other virus annotated CYDV identified proso millet was shown to have relatively low identities compared to CYDV previously reported, suggesting that the virus might be new member of Polerovirus.

The Detection of Plant Viruses in Korean Ginseng (Panax ginseng) through RNA Sequencing

“…Due to the detection limit of conventional diagnostic techniques (PCR and serological assay), it has not yet been clear what kind of virus infects P. ginseng and little is known about plant virus in P. ginseng in Korea. Recently, metatranscriptomic analysis with RNA sequencing (RNA-Seq) and RT-PCR have been used in diagnostic tools for detecting novel or unknown viruses and the confirmation of the presence of viruses (Min et al, 2017, Oh et al, 2018, Yang et al, 2019. This study reports the first metatranscriptomic analysis of the P. ginseng virome using RNA-Seq and RT-PCR assay for identifying the occurrence and incidence of P. ginseng viruses in Korea.…”