A survey of viruses infecting Rosa spp. in New Zealand

Milleza, E. J. M.; Ward, L. I.; Delmiglio, Catia; Tang, Joe; Veerakone, S.; Perez‐Egusquiza, Z.

doi:10.1007/s13313-012-0191-x

Cited by 22 publications

(17 citation statements)

References 29 publications

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“…The bizarre symptoms of WRLRD are clearly different from those associated with known diseases, with no other viruses infecting roses or wild roses having been reported as responsible for WRLRD (Laney et al ., ; Milleza et al ., ; Moury et al ., ; Rivera and Engel, ). Therefore, to our knowledge, this is the first description of the disease.…”

Section: Discussionmentioning

confidence: 99%

“…More than 24 viruses belonging to 10 genera of eight families have been reported to infect roses or wild roses, but no closteroviruses or betaflexiviruses are known to infect Rosa plants (Milleza et al ., ). To our knowledge, this is the first report in this respect.…”

Section: Discussionmentioning

confidence: 99%

“…The rose (Rosa rugosa Thunb.) is one of the most important cut flowers and domestic flowers in the world, and is the top-selling flower in many countries (Milleza et al, 2013). The wild rose (Rosa multiflora Thunb.)…”

Section: Introductionmentioning

confidence: 99%

“…In some countries, virus-indexing procedures are often carried out to ensure that rootstocks are free of virus, but, despite this, viruses remain a problem for the industry. Viral diseases are seldom lethal, but can significantly reduce plant vigour, flower quality and production yield (Milleza et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Deep sequencing reveals a novel closterovirus associated with wild rose leaf rosette disease

Yang

Hóng

et al. 2014

Molecular Plant Pathology

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A bizarre virus-like symptom of a leaf rosette formed by dense small leaves on branches of wild roses (Rosa multiflora Thunb.), designated as 'wild rose leaf rosette disease' (WRLRD), was observed in China. To investigate the presumed causal virus, a wild rose sample affected by WRLRD was subjected to deep sequencing of small interfering RNAs (siRNAs) for a complete survey of the infecting viruses and viroids. The assembly of siRNAs led to the reconstruction of the complete genomes of three known viruses, namely Apple stem grooving virus (ASGV), Blackberry chlorotic ringspot virus (BCRV) and Prunus necrotic ringspot virus (PNRSV), and of a novel virus provisionally named 'rose leaf rosette-associated virus' (RLRaV). Phylogenetic analysis clearly placed RLRaV alongside members of the genus Closterovirus, family Closteroviridae. Genome organization of RLRaV RNA (17,653 nucleotides) showed 13 open reading frames (ORFs), except ORF1 and the quintuple gene block, most of which showed no significant similarities with known viral proteins, but, instead, had detectable identities to fungal or bacterial proteins. Additional novel molecular features indicated that RLRaV seems to be the most complex virus among the known genus members. To our knowledge, this is the first report of WRLRD and its associated closterovirus, as well as two ilarviruses and one capilovirus, infecting wild roses. Our findings present novel information about the closterovirus and the aetiology of this rose disease which should facilitate its control. More importantly, the novel features of RLRaV help to clarify the molecular and evolutionary features of the closterovirus.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Deep sequencing reveals a novel closterovirus associated with wild rose leaf rosette disease

Yang

Hóng

et al. 2014

Molecular Plant Pathology

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“…Cryptoviruses are reported to be widespread in nature and have escaped detection for many years because most of them cause no visible symptoms or, in a few situations, very mild symptoms (Hull 2014). RoCV1 has been found in asymptomatic plants as well as plants with mottling, leaf spots and necrosis (Milleza et al 2013), as well as in mixed infections with rose spring dwarf-associated virus (Salem et al 2008) or rose rosette emaravirus (Martin and Tzanetakis 2008).…”

mentioning

confidence: 99%

High throughput sequencing and RT-qPCR assay reveal the presence of rose cryptic virus-1 in the United Kingdom

et al. 2019

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Rose cryptic virus-1 (RoCV1) also known as Rosa multiflora cryptic virus is a partitivirus affecting roses, one of the most important ornamental crops worldwide. RoCV1 has previously been reported in the US, Canada and New Zealand, and has now been identified in the United Kingdom for the first time. Using High Throughput Sequencing (HTS) RoCV1 sequences were found in two samples collected in 2007 and 2012. This discovery led to the development of a RT-qPCR (TaqMan) assay for the detection of this virus. As part of a rose virus survey in the UK, 251 samples were analysed using the newly developed RoCV1 RT-qPCR test, following ELISA analysis for other common rose viruses. The results of the RT-qPCR test were confirmed using published conventional PCR primers and Sanger sequencing of amplified products. Results suggest that RoCV1 could have been infecting roses in the UK since at least 2007, with a large number of recently collected samples (43%) found to be infected. Cryptoviruses are not thought to cause direct economic losses in their plant host, although it is not clear what impact they might have in mixed infections.Open Access This article is distributed under the terms of the Creative Comm ons Attribution 4.0 International License (http:// creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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