“…Pap sampling from the vaginal pool became obsolete after Ayre (1947) described a modified technique whereby the Pap sample was collected from the ectocervix rather than the vaginal pool. Although Ayre's technique is still used today, recent studies have refuted the belief that use of a small amount of aqueous vaginal lubricant, applied either on the vaginal speculum or vaginal introitus, contaminates the interpretation of cervical cytology, or hampers the detection of cervical human papillomavirus (HPV), Chlamydia trachomatis, and Neisseria gonorrheoeae or bacterial vaginosis (Amies, Miller, Lee, & Koutsky, 2002;Griffith, Stuart, Gluck, & Heartwell, 2005;Hathaway, 2006;Kozakis, Vuddamalay, & Munday, 2006;Sawaya et al, 2008;Vella, 1991). Harmanli and Jones (2010) reported that there is level 1 evidence that a small amount of vaginal lubricant on the external surface of the speculum does not interfere with interpretation of cervical cytology or culture specimens collected from the cervix.…”