In the fast-paced development of life in today’s society, the use of ECG signals is not only used in the medical direction to scan patients; portable ECG signal acquisition tools can collect and analyze the corresponding ECG signals in time and respond to the wearer in time, so that the user can make timely adjustments or seek medical treatment in time to avoid a malignant situation that occurs. For people in sports, the collection of ECG signals is more helpful to the arrangement and estimation of training conditions. Based on nonvolatile memory technology, data will not be lost when the computer is turned off or suddenly or unexpectedly shut down. This research puts forward the design of an antiexercise ECG monitoring system that collects ECG signals for a group that is in the process of exercise. Use mathematical calculation system to classify, and conduct in-depth analysis of the reasons for poor anti-interference ability, and use the original denoising algorithm calculation as the basis to adapt to the dynamic situation in the opposite situation to the normal ECG signal acquisition in the static state. The collection is modified from the original basis. This research proposes an improved scheme for multichannel acquisition. Chips of semiconductor memory devices based on nonvolatile memory are embedded in mobile devices for use. The experimental results in this paper show that the comparison experiment ratio of collecting ECG signals from the sixth, third, fourth, and fifth directions can effectively reduce the mean square error (MSE), and the error of channel 6 is directly from 0.1598. It is down to 0.00143. Several other items showed a significant decrease. It shows that the anti-interference ability of the ECG monitoring system can be further enhanced by increasing the number of collection terminals.