A sustained-release PDGF-BB nanocomposite hydrogel for DM-associated bone regeneration

Li, Yaxin; Liu, Ziyang; Xu, Chenci; Shin, Airi; Wu, Jian‐Ying; Li, Dejian; Lin, Kaili; Liu, Jiaqiang

doi:10.1039/d2tb02037h

Cited by 15 publications

(9 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a similar vein, Li and his colleagues developed a nanocomposite hydrogel containing synthetic magnesium silicate clay that gradually releases PDGF-BB. Despite the regular and gradual release profile, more than 60% of the growth factor is released within the first 5 days . In contrast, our scaffold releases a comparable amount of growth factor within 10 days, making it available for cell differentiation into keratinocytes.…”

Section: Discussionmentioning

confidence: 90%

“…Despite the regular and gradual release profile, more than 60% of the growth factor is released within the first 5 days. 94 In contrast, our scaffold releases a comparable amount of growth factor within 10 days, making it available for cell differentiation into keratinocytes. Additionally, Wei et al utilized a PLGA copolymer to encapsulate platelet growth factor and the PDGF-BB-release profile of these polymer microspheres was also favorable.…”

Section: Discussionmentioning

confidence: 98%

See 1 more Smart Citation

Development of a Composite Hydrogel Containing Statistically Optimized PDGF-Loaded Polymeric Nanospheres for Skin Regeneration: In Vitro Evaluation and Stem Cell Differentiation Studies

Hazrati,

Alizadeh,

Soltani

et al. 2024

ACS Omega

View full text Add to dashboard Cite

Platelet-derived growth factor-BB (PDGF-BB) is a polypeptide growth factor generated by platelet granules faced to cytokines. It plays a role in forming and remodeling various tissue types, including epithelial tissue, through interaction with cell-surface receptors on most mesenchymal origin cells. However, it breaks down quickly in biological fluids, emphasizing the importance of preserving them from biodegradation. To address this challenge, we formulated and evaluated PDGF-encapsulated nanospheres (PD@PCEC) using polycaprolactone-polyethylene glycol-polycaprolactone. PD@PCECs were fabricated through the triple emulsion methodology and optimized by using the Box− Behnken design. The encapsulation efficiency (EE) of nanoencapsulated PDGF-BB was investigated concerning four variables: stirring rate (X1), stirring duration (X2), poly(vinyl alcohol) concentration (X3), and PDGF-BB concentration (X4). The selected optimized nanospheres were integrated into a gelatin-collagen scaffold (PD@PCEC@GC) and assessed for morphology, biocompatibility, in vitro release, and differentiation-inducing activity in human adipose-derived stem cells (hADSCs). The optimized PD@PCEC nanospheres exhibited a particle size of 177.9 ± 91 nm, a zeta potential of 5.2 mV, and an EE of 87.7 ± 0.44%. The release profile demonstrated approximately 85% of loaded PDGF-BB released during the first 360 h, with a sustained release over the entire 504 h period, maintaining bioactivity of 87.3%. The study also included an evaluation of the physicochemical properties of the scaffolds and an assessment of hADSC adhesion to the scaffold's surface. Additionally, hADSCs cultivated within the scaffold effectively differentiated into keratinocyte-like cells (KLCs) over 21 days, evidenced by morphological changes and upregulation of keratinocyte-specific genes, including cytokeratin 18, cytokeratin 19, and involucrin, at both transcriptional and protein levels.

show abstract

Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 98%

Development of a Composite Hydrogel Containing Statistically Optimized PDGF-Loaded Polymeric Nanospheres for Skin Regeneration: In Vitro Evaluation and Stem Cell Differentiation Studies

Hazrati,

Alizadeh,

Soltani

et al. 2024

ACS Omega

View full text Add to dashboard Cite

show abstract

“… 199 Commonly used growth factors include bone morphogenetic protein (BMP), platelet-derived growth factor (PDGF), and fibroblast growth factor (FGF). 195 , 200 Often, growth factors are used in combination with other bioactive molecules to better promote osteogenesis, thereby accelerating the recovery and repair of bone tissue. 201 Ratanavaraporn et al evaluated the effectiveness of gelatin hydrogels doped with a combination of stromal cell-derived factor-1 (SDF-1) and BMP-2 on the regeneration of bone from rat ulna adventitial defects and subcutaneous sites.…”

Section: Tissue Engineering Treatment Of Bone Infectionsmentioning

confidence: 99%

Prospects and challenges for the application of tissue engineering technologies in the treatment of bone infections

Qin,

Yang,

Zhao

et al. 2024

Bone Res

Self Cite

View full text Add to dashboard Cite

Osteomyelitis is a devastating disease caused by microbial infection in deep bone tissue. Its high recurrence rate and impaired restoration of bone deficiencies are major challenges in treatment. Microbes have evolved numerous mechanisms to effectively evade host intrinsic and adaptive immune attacks to persistently localize in the host, such as drug-resistant bacteria, biofilms, persister cells, intracellular bacteria, and small colony variants (SCVs). Moreover, microbial-mediated dysregulation of the bone immune microenvironment impedes the bone regeneration process, leading to impaired bone defect repair. Despite advances in surgical strategies and drug applications for the treatment of bone infections within the last decade, challenges remain in clinical management. The development and application of tissue engineering materials have provided new strategies for the treatment of bone infections, but a comprehensive review of their research progress is lacking. This review discusses the critical pathogenic mechanisms of microbes in the skeletal system and their immunomodulatory effects on bone regeneration, and highlights the prospects and challenges for the application of tissue engineering technologies in the treatment of bone infections. It will inform the development and translation of antimicrobial and bone repair tissue engineering materials for the management of bone infections.

show abstract

“…Accordingly, some therapeutic intervention approaches to facilitate bone healing under diabetic conditions have been proposed, including local application of anti‐inflammatory cytokines, hyperbaric oxygen therapy or implantation of isogenic adult stem cells (ASCs) 7–9 . Unfortunately, bone regeneration of DM remains a clinical challenge, with defect of stem cells in a high‐glucose microenvironment being the primary obstacle 10 . Hence, it is imperative to develop an effective strategy to recruit autologous stem cells to improve osteogenesis in DM patients.…”

Section: Introductionmentioning

confidence: 99%

Targeting RORα in macrophages to boost diabetic bone regeneration

et al. 2023

View full text Add to dashboard Cite

Diabetes mellitus (DM) has become a serious threat to human health. Bone regeneration deficiency and nonunion caused by DM is perceived as a worldwide epidemic, with a very high socioeconomic impact on public health. Here, we find that targeted activation of retinoic acid‐related orphan receptor α (RORα) by SR1078 in the early stage of bone defect repair can significantly promote in situ bone regeneration of DM rats. Bone regeneration relies on the activation of macrophage RORα in the early bone repair, but RORα of DM rats fails to upregulation as hyperglycemic inflammatory microenvironment induced IGF1‐AMPK signalling deficiency. Mechanistic investigations suggest that RORα is vital for macrophage‐induced migration and proliferation of bone mesenchymal stem cells (BMSCs) via a CCL3/IL‐6 depending manner. In summary, our study identifies RORα expressed in macrophages during the early stage of bone defect repair is crucial for in situ bone regeneration, and offers a novel strategy for bone regeneration therapy and fracture repair in DM patients.

show abstract

A sustained-release PDGF-BB nanocomposite hydrogel for DM-associated bone regeneration

Abstract: Regeneration of bone tissue in the environment of diabetes mellitus (DM) remains one of the clinical challenges, with malfunction of stem cells in a high-glucose microenvironment being the primary obstacle....

Cited by 15 publications

References 35 publications

Development of a Composite Hydrogel Containing Statistically Optimized PDGF-Loaded Polymeric Nanospheres for Skin Regeneration: In Vitro Evaluation and Stem Cell Differentiation Studies

Development of a Composite Hydrogel Containing Statistically Optimized PDGF-Loaded Polymeric Nanospheres for Skin Regeneration: In Vitro Evaluation and Stem Cell Differentiation Studies

Prospects and challenges for the application of tissue engineering technologies in the treatment of bone infections

Targeting RORα in macrophages to boost diabetic bone regeneration

Contact Info

Product

Resources

About