A synthesis of cyclo-2,3-diphospho-D-glycerate from D-mannitol

Berkessel, Albrecht; Geisel, Urs; Hérault, David A.

doi:10.1016/0040-4039(95)02154-x

Cited by 3 publications

(2 citation statements)

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“…Phospho compounds, enzymes, and coenzymes Cyclic 2,3-diphosphoglycerate (ammonium salt) was synthesized in an enantiomerically pure form from D-mannitol as described [Berkessel et al 1996; see also Earle et al (1996)]. 2,3-Bisphosphoglycerate (pentasodium salt) was from Sigma (Deisenhofen, Germany).…”

Section: Methodsmentioning

confidence: 99%

Activation and thermostabilization effects of cyclic 2,3-diphosphoglycerate on enzymes from the hyperthermophilic Methanopyrus kandleri

Shima¹,

Hérault

Berkessel

et al. 1998

Archives of Microbiology

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Enzymes involved in methane formation from carbon dioxide and dihydrogen in Methanopyrus kandleri require high concentrations (> 1 M) of lyotropic salts such as K2HPO4/KH2PO4 or (NH4)2SO4 for activity and for thermostability. The requirement correlates with high intracellular concentrations of cyclic 2,3-diphosphoglycerate (cDPG; approximately 1 M) in this hyperthermophilic organism. We report here on the effects of potassium cDPG on the activity and thermostability of the two methanogenic enzymes cyclohydrolase and formyltransferase and show that at cDPG concentrations prevailing in the cells the investigated enzymes are highly active and completely thermostable. At molar concentrations also the potassium salts of phosphate and of 2,3-bisphosphoglycerate, the biosynthetic precursor of cDPG, were found to confer activity and thermostability to the enzymes. Thermodynamic arguments are discussed as to why cDPG, rather than these salts, is present in high concentrations in the cells of Mp. kandleri.

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Section: Methodsmentioning

confidence: 99%

Activation and thermostabilization effects of cyclic 2,3-diphosphoglycerate on enzymes from the hyperthermophilic Methanopyrus kandleri

Shima¹,

Hérault

Berkessel

et al. 1998

Archives of Microbiology

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“…However, carbodiimide coupling of 7 smoothly led to the formation of the protected cyclic pyrophosphate 9 . In these reactions, the carbodiimide used was not dicyclohexylcarbodiimide as in the synthesis of the cyclic pyrophosphate methanophosphagen, but rather a large excess of the water-soluble carbodiimide EDC . The substituted urea byproduct of the reaction was removed by cation exchange chromatography (DOWEX 50WX8-200).…”

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confidence: 99%