2015
DOI: 10.1016/j.ymben.2015.03.016
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A synthetic cGMP-sensitive gene switch providing Viagra®-controlled gene expression in mammalian cells and mice

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Cited by 10 publications
(3 citation statements)
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“…The activity of this chimera is tightly regulated by the hormone b-estradiol and the intensity of the response is modulated by the number of lexA-binding sites in the target promoter allowing the expression levels to be scaled up or down. In general, network motifs can be implemented using different tools such as synthetic promoters, 15,[92][93][94][95] synthetic transcription factors 96,97 and synthetic RNA biosensors or riboswitches. [98][99][100] Besides cooperativity, molecular titration 101 This journal is © The Royal Society of Chemistry 2016 can give rise to ultrasensitivity.…”
Section: Digital Synthetic Circuitsmentioning
confidence: 99%
“…The activity of this chimera is tightly regulated by the hormone b-estradiol and the intensity of the response is modulated by the number of lexA-binding sites in the target promoter allowing the expression levels to be scaled up or down. In general, network motifs can be implemented using different tools such as synthetic promoters, 15,[92][93][94][95] synthetic transcription factors 96,97 and synthetic RNA biosensors or riboswitches. [98][99][100] Besides cooperativity, molecular titration 101 This journal is © The Royal Society of Chemistry 2016 can give rise to ultrasensitivity.…”
Section: Digital Synthetic Circuitsmentioning
confidence: 99%
“…One such system, developed by Kim et al, detected intracellular cGMP levels and produced an easily-discernable output upon detection. 50 To detect the cGMP surge following signalling, they took advantage of a natural non-human cGMP sensor and rewired it to act as an inducible transcription factor. 50 They showed that the system specifically reacted to cGMP in a graded manner.…”
Section: Detecting Intra-and Extra-cellular Statesmentioning
confidence: 99%
“…50 To detect the cGMP surge following signalling, they took advantage of a natural non-human cGMP sensor and rewired it to act as an inducible transcription factor. 50 They showed that the system specifically reacted to cGMP in a graded manner. cGMP is normally degraded by phosphodiesterases (PDEs), and the inhibition of PDEs to prolong cGMP signalling is a clinically applied therapy.…”
Section: Detecting Intra-and Extra-cellular Statesmentioning
confidence: 99%