A synthetic curcuminoid derivative inhibits nitric oxide and proinflammatory cytokine synthesis

Tham, Chau Ling; Liew, Choi Yi; Lam, Kok Wai; Mohamad, Azam Shah; Kim, Min Kyu; Cheah, Yoke Kqueen; Zakaria, Zuhaina; Sulaiman, Mohd Roslan; Lajis, Nordin H.; Israf, Daud Ahmad

doi:10.1016/j.ejphar.2009.11.053

Cited by 59 publications

(37 citation statements)

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“…As described previously, curcumin had some targets, such as growth factors, growth factor receptors, transcription factors, cytokines, enzymes, and apoptosis regulator genes. 38,39,42,43 Gautam and Jachak 44 demonstrated decreased levels of IL-1b and TNF-a in mice suffering from chronic inflammation. By in vitro study, it is shown that a level of 5mM curcumin at can inhibit the production of TNF-a and IL-1 by human monocyte induced by Lipopolysaccharide (LPS).…”

Section: Figurementioning

confidence: 98%

Curcumin decreased level of proinflammatory cytokines in monocyte cultures exposed to preeclamptic plasma by affecting the transcription factors NF-κB and PPAR-γ

Rahardjo

Widjajanto

Sujuti

et al. 2014

Biomarkers and Genomic Medicine

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The purpose of this study is to determine the role of curcumin in the alteration of levels of interleukin (IL)-1a, IL-6, and tumor necrosis factor a (TNF-a) as proinflammatory cytokines in monocyte culture exposed to preeclamptic plasma, as well as the effect on the transcription factors: nuclear factor kappa beta (NF-kB) and peroxisome proliferator-activated receptor g (PPAR-g). Plasma was taken from preeclamptic women (n Z 12) and normotensive pregnant women (n Z 12). Monocyte cultures were taken from nonpregnant healthy woman. Monocyte cultures were incubated with plasma for 48 hours. Curcumin in various doses were given in monocytic cultures prior to and after preeclamptic plasma exposure. The levels of IL-1a, IL-6, and TNF-a as well as NF-kB and PPAR-g in each culture were determined by enzyme-linked immunosorbent assay procedures. The final data were analyzed by analysis of variance (ANOVA) and path analysis. This study shows a significant increase (p < 0.05) in the levels of proinflammatory cytokines (IL-1a, IL-6, and TNFa) in monocyte cultures exposed (B.Rahardjo). + MODELPlease cite this article in press as: Rahardjo B, et al., Curcumin decreased level of proinflammatory cytokines in monocyte cultures exposed to preeclamptic plasma by affecting the transcription factors NF-kB and PPAR-g, Biomarkers and Genomic Medicine (2014), http://dx. Biomarkers and Genomic Medicine (2014) xx, 1e11 to preeclamptic plasma compared with normotensive pregnancy plasma. Curcumin treatment in various doses can decrease significantly (p < 0.05) proinflammatory cytokine levels in monocyte cultures that have been already stimulated by preeclamptic plasma. After curcumin treatment, there was a decreased level of nuclear NF-kB p50 and a significantly increased level of PPAR-g. Curcumin has a direct effect on decreasing the levels of nuclear NF-kB p50 and also curcumin indirectly influenced the level of nuclear NF-kB p50 by the increased level of PPAR-g. Curcumin could decrease levels of proinflammatory cytokines (IL-1a, IL-6, and TNFa) in monocyte cultures exposed to preeclamptic plasma by affecting the transcription factors, NF-kB and PPAR-g. Curcumin has potential in the prevention and future treatment of preeclampsia, through inflammation pathways assumed as being responsible for the development of preeclampsia.

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Section: Figurementioning

confidence: 98%

Curcumin decreased level of proinflammatory cytokines in monocyte cultures exposed to preeclamptic plasma by affecting the transcription factors NF-κB and PPAR-γ

Rahardjo

Widjajanto

Sujuti

et al. 2014

Biomarkers and Genomic Medicine

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“…The amount of nitrite was extrapolated from the sodium nitrite (Sigma Aldrich) standard graph. The inhibition rate was calculated as described previously by Tham et al (2010).…”

Section: Nitrite Determinationmentioning

confidence: 99%

“…The protein concentration in the cell lysate was determined using the Pierce Bicinchoninic Acid (BCA) Protein Assay kit (Thermo Fisher Scientific) as described by the manufacturer. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot were performed as described by Tham et al (2010). To detect iNOS and β-actin, the membranes (Pall Corporation, Port Washington, NY, USA) were incubated separately in antiiNOS rabbit polyclonal primary antibody (1:1000 dilution) (Cayman Chemical Company, Ann Arbor, MI, USA) and anti-β-actin polyclonal antibody (1:1000 dilution) (BioVision Incorporated, Milpitas, CA, USA) overnight.…”

Section: Western Blot Analysismentioning

confidence: 99%

Inhibition of nitric oxide production by Solanum melongena and Solanum macrocarpon on RAW 264.7 cells

Yiin

Abidin

Shuib

et al. 2015

Frontiers in Life Science

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Nitric oxide (NO) plays an important role in the physiology and pathophysiology of disease. Overproduction of NO is associated with chronic inflammatory diseases and cancers. Several species of Solanaceae have been used traditionally to treat inflammatory-related diseases. To analyse the possible anti-inflammatory properties of these species, the Griess assay was used to evaluate the effects of various Solanum melongena and Solanum macrocarpon extracts on NO production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. The cytotoxicity of the extracts on the cell line was tested using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Extracts that significantly inhibited NO production were further evaluated for inducible nitric oxide synthase (iNOS) expression by Western blot. Thin-layer chromatography was used to determine the major compounds in the extracts. All extracts significantly inhibited NO production in LPS-stimulated RAW 264.7 cells in a dose-dependent manner. At 200 μg/ml, ethyl acetate extract of S. macrocarpon showed the highest NO inhibition of 81%, with a median inhibitory concentration (IC 50 ) value of 44.78 ± 0.04 μg/ml. The viability of cells treated with the extracts was greater than 80%. Ethanol and ethyl acetate extracts of S. melongena, together with ethanol, hexane and ethyl acetate extracts of S. macrocarpon, reduced iNOS expression significantly. At 200 μg/ml, ethyl acetate extract of S. macrocarpon inhibited iNOS protein expression by 79%. Phytochemical analysis of the extracts showed that fluorescent, double-bond compounds, phenols, flavonoids and terpenoids were mainly present in the extracts. Taken together, the results show the potential of ethanol and ethyl acetate extracts of S. melongena, and hexane and ethyl acetate extracts of S. macrocarpon, as agents for the prevention and treatment of inflammatory-related diseases.

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“…There are many attempts to synthesize curcumin analogues to circumvent the problem of low bioavailabilities and extensive metabolism (Anand et al 2007(Anand et al , 2008. In terms of the potential pharmacodynamic effects of curcumin, anti-inflammatory (Tham et al 2010;Zhao et al 2010) and antioxidant activities (Dai et al 2009) are two of the most prominent properties among all others. Interestingly, some analogues have independent anti-inflammatory activities that are not associated with the "antioxidant" activities (Lee et al 2009).…”

Section: Polyphenols: Curcumin and Curcmin Analoguesmentioning

confidence: 99%

Role of Nutraceuticals on Nrf2 and Its Implication in Cancer Prevention

Saw

Kong

2011

Nutraceuticals and Cancer

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"Nutraceuticals" have recently become a hot topic for the commercial world and the biomedical community. It is postulated that nutraceuticals are relatively non-toxic food supplements with many health benefits including prevention of cancer. Many nutraceuticals are antioxidants by nature; isothiocyanates and polyphenols have been extensively researched in many laboratories including ours. This chapter will cover nutraceuticals from the mechanistic perspectives, and how they would interact with Nuclear factor (erythroid-derived 2)-like 2 (Nrf2), which is the master regulator of the antioxidant response to counteract oxidative stress. Nrf2 deficient (knockout; KO) mice lack response to some nutraceutical cancer chemopreventive agents suggesting the important role of Nrf2 in cancer prevention. Nrf2 is also shown to have protective function against inflammation, a pathological process that is implicated in many diseases including carcinogenesis. Scientific evidence on the role of nutraceuticals on Nrf2 in anti-oxidative stress coupled with anti-inflammatory will also be discussed in this chapter.

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A synthetic curcuminoid derivative inhibits nitric oxide and proinflammatory cytokine synthesis

Cited by 59 publications

References 31 publications

Curcumin decreased level of proinflammatory cytokines in monocyte cultures exposed to preeclamptic plasma by affecting the transcription factors NF-κB and PPAR-γ

Curcumin decreased level of proinflammatory cytokines in monocyte cultures exposed to preeclamptic plasma by affecting the transcription factors NF-κB and PPAR-γ

Inhibition of nitric oxide production by Solanum melongena and Solanum macrocarpon on RAW 264.7 cells

Role of Nutraceuticals on Nrf2 and Its Implication in Cancer Prevention

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