“…There are, however, a number of Salmonellae which are unable to synthesize certain essential vitamins and amino acids and these must be supplied in the culture medium. The need of Salmonella typhosa for tryptophan (Fildes et al, 1933), of Salmonella gallinarum for vitamin B1, and of Salmonella pullorum for nicotinic acid, leucine, aspartic acid, cystine, and other amino acids (Johnson and Rettger, 1943;Gilfillan et al, 1955) has been described. Also, Lederberg (1947) has reported strains of Salmonella paratyphi A, S. paratyphi B, S. choleraesuis, S. enteritidis, S. dublin, and S. typhimurium which require for growth different combinations of vitamin B1, biotin, and a variety of amino acids.…”