A system employing a minimal defined medium for the selection of tumorigenic cells

Schaeffer, Warren I.; Simkins, Stephen G.; Lamore, Bette J.

doi:10.1007/bf02624431

Cited by 5 publications

(2 citation statements)

References 7 publications

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“…Although most tumours are of monoclonal origin, they are composed of multiple cell subpopulations which differ in characteristics such as: nutritional requirements [5], metastatic potential, growth kinetics [29], sensitivity to host defense mechanisms, and immunogenicity [30]. LB leukaemia may be constituted by several cell populations with different immunogenic characteristics.…”

Section: Discussionmentioning

confidence: 99%

“…Tumour cell populations are often characterized by their heterogeneity; since genetic instability and epigenetic or phenotypic modulation allow variants to develop . Under some circumstances, changes in the composition of the microenvironment of tutnour cells may exert selective pressure, resulting in populations with advantageous alterations in their properties [5].…”

Section: Introductionmentioning

confidence: 99%

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Induction of Anti‐Tumour Immunity in Syngeneic Mice by Leukaemic Cell Line

et al. 1995

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Induction of anti-tumour immunity in syngeneic mice by LBC cell line derived from a non-immunogenic T cell leukaemia was studied. The immunization of BALB/c mice with LBC irradiated cells induced in them anti-tumour spleen cells, cytotoxic T lymphocytes and anti-LBC antibodies. The anti-LBC antibodies reacted with components of 14, 16 and 27 kDa present on LB tumour cells, LBC cell line and normal thymocytes, but not with normal lymph node cells. Furthermore, immunization of the autologous hosts with LBC cells partially protected them against subsequent challenge with the original LB leukaemic cells. These findings demonstrate that culture conditions induced modifications in the antigenic properties of the leukaemic cells, allowing LBC cells to stimulate an immune response directed against components expressed at early stages during T cell maturation. These results also suggest that the immune response is responsible for the prolongation of the survival time of the mice inoculated with the parental leukaemic cells.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Induction of Anti‐Tumour Immunity in Syngeneic Mice by Leukaemic Cell Line

et al. 1995

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Effects of subcultivation and culture medium on differentiation of human fetal cardiac myocytes

Goldman

Wurzel

1992

In Vitro Cell Dev Biol - Animal

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Previous work has suggested that subcultivated human fetal heart muscle cell cultures contain immature cardiac muscle cells capable only of limited differentiation after mitogen withdrawal. We studied several human fetal heart cultures (14-15 wk gestation) at several passage levels using immunocytochemistry, autoradiography, and Northern blot analysis. Characteristics in high-mitogen (growth) medium were compared with those after serum withdrawal. Cultured cells from one heart, expanded through 2 passages in growth medium, did not beat; however, 75% of cells did beat after subsequent culture for 24 days in low-serum (differentiation) medium containing insulin. In confluent cultures after 1 passage, there was no detectable difference in the number of cardiac myocytes present in growth medium compared with that 7 days after serum withdrawal. After 4 passages, however, serum withdrawal increased the number of cells expressing immunoreactive sarcomeric myosin heavy chain by 100-fold; expression of immunoreactive sarcomeric actin and alpha-cardiac actin mRNA also increased in the same cultures. Similar results were obtained in cultures kept in differentiation medium for 20 days before passage and expansion in growth medium. Using isopycnic centrifugation, a high-density cell fraction was isolated which contained no immunostained myocytes in growth medium but numerous myocytes after serum withdrawal. Combined immunocytochemistry/autoradiography showed that myocytes synthesize DNA in growth medium and in serum-free medium containing fibroblast growth factor, but not in serum-free medium alone. The results indicate that a) human fetal cardiac muscle cells proliferate in vitro and can maintain a phenotype characteristic of fetal myocytes after multiple subcultivations followed by serum withdrawal; b) after subcultivation in growth medium, some myocytes modulate their phenotype into one in which detectable levels of cardiac contractile proteins are expressed only after mitogen withdrawal, and c) the phenotype attained after serum withdrawal is in part dependent on passage level. Cultured human fetal myocardial cells may provide a useful experimental system for the study of human cardiac muscle cell biology.

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Replacement of vertebrate serum with lipids and other factors in the culture of invertebrate cells, tissues, parasites, and pathogens

Goodwin

1991

In Vitro Cell Dev Biol - Animal

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Culture medium supplementation with vertebrate serum results in the selection of fibroblastoid insect cell lines and a general decline during continuous subculturing of both morphologic and functional differentiation of the surviving cells. Essential lipid mixtures can substitute for vertebrate serum in the culture of insect and some vertebrate cells, tissues, parasites, and pathogens. The provision of sterols and essential (with nonessential) polyunsaturated fatty acids as phospholipids in oxidation-protected peptoliposomes or proteoliposomes allows cells in culture to duplicate in vivo specific membranes more accurately. Such lipid-corrected membranes allow cultured cells to communicate with neighboring cells through the extracellular matrix, effectively transmit hormonal signals directly and via receptor control, and respond with various tissue-specific functions and differentiation states as directed.

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A system employing a minimal defined medium for the selection of tumorigenic cells

Cited by 5 publications

References 7 publications

Induction of Anti‐Tumour Immunity in Syngeneic Mice by Leukaemic Cell Line

Induction of Anti‐Tumour Immunity in Syngeneic Mice by Leukaemic Cell Line

Effects of subcultivation and culture medium on differentiation of human fetal cardiac myocytes

Replacement of vertebrate serum with lipids and other factors in the culture of invertebrate cells, tissues, parasites, and pathogens

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