2020
DOI: 10.1186/s12906-020-2871-3
|View full text |Cite
|
Sign up to set email alerts
|

A systematic analysis of natural α-glucosidase inhibitors from flavonoids of Radix scutellariae using ultrafiltration UPLC-TripleTOF-MS/MS and network pharmacology

Abstract: Background: Flavonoids from plant medicines are supposed to be viable alternatives for the treatment of type 2 diabetes (T2D) as less toxicity and side effects. Radix scutellariae (RS) is a widely used traditional medicine in Asia. It has shown great potential in the research of T2D. However, the pharmacological actions remain obscured due to the complex chemical nature of plant medicines.Methods: In the present study, a systematic method combining ultrafiltration UPLC-TripleTOF-MS/MS and network pharmacology … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

0
17
0

Year Published

2020
2020
2023
2023

Publication Types

Select...
7
1

Relationship

0
8

Authors

Journals

citations
Cited by 21 publications
(17 citation statements)
references
References 86 publications
0
17
0
Order By: Relevance
“…Hs.eg.db (version 3.8.2; bioconductor.org/packages/org.Hs.eg.db ), for pathway enrichment analysis by the use of the predicted targets. The compound-target-pathway network was then established with the software Cytoscape-v3.7.2 ( Shannon et al, 2003 ) for understanding the complex relationships and revealing the molecular mechanisms ( Wang L. et al, 2020 ).…”
Section: Methodsmentioning
confidence: 99%
“…Hs.eg.db (version 3.8.2; bioconductor.org/packages/org.Hs.eg.db ), for pathway enrichment analysis by the use of the predicted targets. The compound-target-pathway network was then established with the software Cytoscape-v3.7.2 ( Shannon et al, 2003 ) for understanding the complex relationships and revealing the molecular mechanisms ( Wang L. et al, 2020 ).…”
Section: Methodsmentioning
confidence: 99%
“…With this integrated information, the ion was finally confirmed to be that of baicalin (Fig 1). The corresponding mass spectra and related structures are shown in Fig 1. According to the above-mentioned analytical method and relevant literature [8,21], a total of 25 chemical markers that were differentially expressed between the two Radix Scutellariae treatment groups was successfully identified, including 19 candidate ions in positive ion mode and 6 candidate ions in negative mode. Using Waters Masslynx software, we finally confirmed their identities with the MS/MS data.…”
Section: Identification Of Chemical Markers In Radix Scutellariaementioning
confidence: 99%
“…However, these two compounds could be distinguished by the relative abundance of the loss of CO and CHO from the parent ion [M+H‐176‐CH 3 ] + . In the MS/MS analysis of compound 5, the relative abundance of ion [M+H‐176‐CH 3 ‐CO] + at m/z 242.05 was lower than that of ion [M+H‐176‐CH 3 ‐CHO] at m/z 241.04, since the loss of CHO could produce a more stable p ‐quinoid skeleton [26]. Nevertheless, this was contrary to the analysis of compound 4.…”
Section: Resultsmentioning
confidence: 99%