A Systematic Method for Evaluating Quality of Jinqi Jiangtang Tablet: Simultaneous Determination of 21 Compounds by HPLC–MS

Xu, Zhangyao; Zhao, Yining; Qiu, Zhipeng; Li, Yuanyuan; Wang, Tianming; Shi, Rong; Ma, Yueming

doi:10.1007/s10337-018-3667-1

Cited by 2 publications

(1 citation statement)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The chemical components contained in TCM compounds are complex with uneven content, and those effective components are the material basis of the biological effect of the whole formula. Through the simultaneous qualitative and quantitative analysis, the content of various chemical components and the proportion of each component can be determined, hence realizing the quality control of TCM compounds [ 21 – 26 ]. However, to the best of our knowledge, the methods for determining components used in previous reports only focused on one or two herbs of the whole preparation of GGJTW [ 1 , 27 – 29 ].…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of Ultrahigh-Performance Liquid Chromatography Coupled with Triple Quadrupole Mass Spectrometry Method for Quantitative Determination of Ten Active Compounds in Ge-Gen-Jiao-Tai-Wan

Wang

Zhu

Chen

et al. 2022

Journal of Analytical Methods in Chemistry

View full text Add to dashboard Cite

A rapid, accurate, and sensitive method for the simultaneous determination of 10 main components, namely puerarin, daidzin, coptisine, epiberberine, jatrorrhizine, berberine, palmatine, coumarin, daidzein, and cinnamic acid in Ge-Gen-Jiao-Tai-Wan, was developed based on ultra-high-performance liquid chromatography coupled with triple quadrupole mass spectrometry. Analysis was performed on an Agilent 1290 Infinity II series UHPLC system, equipped with a Waters ACQUITY UPLC HSS T3 column (100 × 2.1 mm, 1.8 μm) by using (A) 0.1% acetic acid and (B) methanol as mobile phase. The flow rate was 0.3 mL/min, and the injection volume was 1 μL. Mass spectrometry was operated in multiple reaction monitoring mode using an Agilent 6460 triple quadrupole mass spectrometer equipped with an AJS-ESI ion source. Agilent Mass Hunter Work Station Software was employed for data acquisition and processing. All calibration curves showed excellent linear regressions (R2 > 0.9992). The precision, repeatability, and stability of the ten compounds were below 4.56% in terms of relative standard deviation. The average extraction recovery ranged from 96.53% to 102.69% with a relative standard deviation of 1.14–3.78% for all samples. This study potently contributes to the quantitative evaluation of Ge-Gen-Jiao-Tai-Wan, thereby providing a scientific basis for further studies and clinical application of Ge-Gen-Jiao-Tai-Wan.

show abstract