This study aimed to identify differences in sprint performance outcomes when comparing sustained isometric contractions to plyometric exercise and a non-dynamic warmup. The sample observed were from female collegiate basketball, soccer, and volleyball athletes with prior strength and sprint training experience (n = 8). Over the course of four randomized sessions, the participants performed a non-dynamic control protocol (Con), a plyometric protocol (Plyo), a short duration (10s) lower body isometric protocol (SDI), and a long duration (30s) lower body isometric contraction protocol (LDI). Three sprint trials were performed at one-, five-, and 10minutes post-potentiation protocol. Three hypotheses were made given the protocols: (H1) The submaximal isometric contractions in the lower body would be equal to plyometric exercises as a potentiation tool for 20-meter sprint performance (H2) a longer submaximal contraction (:30) would be superior to the shorter duration isometric contraction (:10) for 20-meter sprint performance (H3) both isometric contraction protocols would be superior to a control warm up that does not include any plyometric or heavy strength stimulus. It was found that there was a significant effect noted for the protocols used in the study [F(3, 21) = 3.99, p = 0.021, partial η 2 = 0.36]. However, no significant effect was found between the different levels of the rest intervals, and no interaction was present between protocol and rest. Additionally, no significant difference