A systematic review and meta-analysis approach on diagnostic value of MLH1 promoter methylation for head and neck squamous cell carcinoma

Li, Qun; Hong, Jinjiong; Shen, Zhisen; Deng, Hongxia; Shen, Yi; Wu, Zhenhua; Zhou, Chongchang

doi:10.1097/md.0000000000017651

Cited by 4 publications

(4 citation statements)

References 19 publications

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“…These differences reflect the heterogeneity of these diseases in their histology and clinical behavior, with different aetiologias and risk factors, and known tissue and tumor-type specificity of methylation patterns [ 22 ]. The prevalence of MGMT and MLH1 methylation detected in this study are also consistent with previously summarized data for HNSC in the TCGA database [ 23 , 24 ].…”

Section: Discussionsupporting

confidence: 92%

DNA Methylation by Bisulfite Next-Generation Sequencing for MLH1 and MGMT in Oral Squamous Cell Carcinomas and Potentially Malignant Disorders: An Integrative Analysis towards Field Cancerization

et al. 2022

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Background and Objectives: MGMT methylation is a well-described biomarker in several solid tumors and MLH1 seems to occur in the initial stages of oral carcinogenesis. The aims of this study were to evaluate MHL1 and MGMT methylation levels in oral squamous cell carcinoma (OSCC) and oral potentially malignant disorders (OPMDs), and to integrate this information with The Cancer Genome Atlas (TCGA) database. Materials and Methods: To determine the percentage of gene methylation in MLH1 and MGMT, pyrosequencing analysis was conducted. Samples were divided as follows: (1) patients diagnosed with OSCC (N = 16); (2) patients with OPDM who developed OSCC in the same location (N = 47); and (3) patients with OPDM who developed OSCC in a different location (N = 22). As a validation cohort in this study, data from The Cancer Genomic Atlas (TCGA) database, particularly regarding Head and Neck Squamous Cell Carcinoma, was used. Results: Overall MLH1 methylation levels of 8.6 ± 11.5% and 8.1 ± 9.2% for MGMT were obtained. With regard to MHL1, the OSCC presented the highest degree of methylation with 9.3 ± 7.3% (95%CI 5.1–13.6), and with regards to MGMT, the simultaneous malignancy group presented the highest degree of methylation with 10 ± 13.5% (95%CI 6–10), although no significant differences were found between the groups (p = 0.934 and p = 0.515, respectively). The estimated survival was higher for MGMT methylated cases (19.1 months, 95%CI 19.1–19.1) than for unmethylated cases (9.4 months, 95%CI 6–12.8), but not statistically significant. Conclusions: Our results did not show a correlation between MGMT and MLH1 methylation and any clinicopathological feature or survival in our institutional cohort. MLH1 methylation was present mainly in OSCC, whilst MGMT in OPMD represented a modest contribution to field cancerization, with an overall consistency with the TCGA database.

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Section: Discussionsupporting

confidence: 92%

DNA Methylation by Bisulfite Next-Generation Sequencing for MLH1 and MGMT in Oral Squamous Cell Carcinomas and Potentially Malignant Disorders: An Integrative Analysis towards Field Cancerization

et al. 2022

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“…The MLH1 promoter methylation has been reported as a well-established biomarker in several types of cancer, such as esophageal cancer, colorectal cancer, non-small cell lung cancer, gastric cancer, papillary thyroid cancer, and bladder cancer [16] .…”

Section: Resultsmentioning

confidence: 99%

Tobacco Smoking as A Risk Factor in DNA Methylation of Repair Gene (MLH1) Using Cytobbrush from Lateral Border of the Tongue

Muayad Hashim Matloob,

Ameena Ryhan Diajil

2021

Indian Journal of Forensic Medicine & Toxicology

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Objective:The aim of this study was to evaluate the epigenetic effect in the process of oral carcinogenesis by screening the methylation of repair gene in chronic tobacco smokers.Material and Method: Study design: One hundred male volunteers, divided into two groups: the 1 st group consisted of 58 smokers, each consumed 20 cigarettes/day for at least 10 years; and the 2 nd group consisted of 48non-smokers who were consider as a control group.The samples were taking from lateral border of the tongue by exfoliative cytology, and the extracted DNA was treated with phenol-chloroform gDNA,Screening of methylation was done by Methyl Specific PCR (MSP).Results :-The results of this studyshowed significant effect of tobacco smoking in methylation of MLH1 gene in site 1in comparison to non-smoker group,(P > 0.05), with Odds ratio = 4.957 CI ().

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“…A meta-analysis has suggested that hypermethylation of the MLH1 promoter is associated with HNSCC. Thus, methylated MLH1 could be a potential diagnostic biomarker for HNSCC (91). One study showed that epigenetic silencing of O6-methylguanine-DNA methyltransferase (MGMT, involved in the DR pathway) DNA repair enzyme through promoter hypermethylation (HmMGMT) may increase TP53 oncosuppressor gene mutations, thereby promoting HNSCC (92).…”

Section: Dna Damage Repair In Hnsccmentioning

confidence: 99%

Targeting DNA damage response as a potential therapeutic strategy for head and neck squamous cell carcinoma

Lei

He²,

Jiang³

et al. 2022

Front. Oncol.

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Cells experience both endogenous and exogenous DNA damage daily. To maintain genome integrity and suppress tumorigenesis, individuals have evolutionarily acquired a series of repair functions, termed DNA damage response (DDR), to repair DNA damage and ensure the accurate transmission of genetic information. Defects in DNA damage repair pathways may lead to various diseases, including tumors. Accumulating evidence suggests that alterations in DDR-related genes, such as somatic or germline mutations, single nucleotide polymorphisms (SNPs), and promoter methylation, are closely related to the occurrence, development, and treatment of head and neck squamous cell carcinoma (HNSCC). Despite recent advances in surgery combined with radiotherapy, chemotherapy, or immunotherapy, there has been no substantial improvement in the survival rate of patients with HNSCC. Therefore, targeting DNA repair pathways may be a promising treatment for HNSCC. In this review, we summarized the sources of DNA damage and DNA damage repair pathways. Further, the role of DNA damage repair pathways in the development of HNSCC and the application of small molecule inhibitors targeting these pathways in the treatment of HNSCC were focused.

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A systematic review and meta-analysis approach on diagnostic value of MLH1 promoter methylation for head and neck squamous cell carcinoma

Cited by 4 publications

References 19 publications

DNA Methylation by Bisulfite Next-Generation Sequencing for MLH1 and MGMT in Oral Squamous Cell Carcinomas and Potentially Malignant Disorders: An Integrative Analysis towards Field Cancerization

DNA Methylation by Bisulfite Next-Generation Sequencing for MLH1 and MGMT in Oral Squamous Cell Carcinomas and Potentially Malignant Disorders: An Integrative Analysis towards Field Cancerization

Tobacco Smoking as A Risk Factor in DNA Methylation of Repair Gene (MLH1) Using Cytobbrush from Lateral Border of the Tongue

Targeting DNA damage response as a potential therapeutic strategy for head and neck squamous cell carcinoma

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