A Systematic Review of Autologous Platelet-Rich Plasma and Fat Graft Preparation Methods

Luck, Joshua; Smith, Oliver J.; Mosahebi, Afshin

doi:10.1097/gox.0000000000001596

Cited by 30 publications

(21 citation statements)

References 77 publications

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“…Certainly, many researchers also reported a similar positive effect of platelet-rich plasma (PRP) addition as an alternative approach in fat grafting. 31 However, for some patients with fat accumulation, extracting SVF from a large number of excess aspirated fat is better than extracting PRP from blood.…”

Section: Discussionmentioning

confidence: 99%

Autologous fat graft assisted by stromal vascular fraction improves facial skin quality: A randomized controlled trial

Yin

et al. 2020

Journal of Plastic, Reconstructive & Aesthetic Surgery

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Background: Cell-assisted lipotransfer (CAL) promotes the survival of fat grafts with high vascular density and improves skin quality by increasing collagen content. However, no study has quantified the changes on the skin surface, and rigorous methodological evaluations are still lacking. Design: Fifty patients were recruited and randomly divided into two groups: an experimental group (n = 25) that underwent a stromal vascular fraction (SVF)-assisted fat graft and a control group (n = 25) that underwent fat graft only. Methods: The SVF cells were counted, tested in terms of viability, and characterized. The volumes of whole faces were determined by using a 3D scanner and Geomagic software preoperation, immediately after surgery, and 6 months postoperation. Facial skin qualities, including spots, wrinkles, texture, pores, UV spots, brown spots, red areas, and porphyrins, were detected by a VISIA skin detector preoperation and 6 months postoperation. A visual analog scale was used for clinical evaluation. Results: The cell pellet contained 1-3 × 10 7 /mL of fresh SVF cells. The cell viability exceeded 98%. The immunophenotyping characteristics and stemness were consistent with the features of adipose-derived stem cells (ADSCs). The survival rate of SVF-enriched fat grafts was significantly higher than that of control grafts: 77.6% ±11.6% versus 56.2% ±9.5% (p < 0.001). The VISIA values of wrinkles (19.3 ± 6.6 versus 10.9 ± 5.5, p < 0.001) and texture (15.8 ± 7.0 versus 10.3 ± 5.0, p < 0.01) were significantly higher in SVF-enriched group than in control group at 6 months postoperation. During long-term follow-up, the majority of patients in both groups were satisfied with the final facial esthetic results.

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Section: Discussionmentioning

confidence: 99%

Autologous fat graft assisted by stromal vascular fraction improves facial skin quality: A randomized controlled trial

Yin

et al. 2020

Journal of Plastic, Reconstructive & Aesthetic Surgery

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“…Due to early ischemia and hypoxia, adipose tissue with a diameter larger than 1200 um tend to necrosis at the central region after transplantation into the body 8 . Studies showed that implanted fat tissue can be classified into three zones from the periphery to the center at 4 weeks after transplantation: survival, regenerated, and necrotic zones 9 .…”

mentioning

confidence: 99%

Delayed two steps PRP injection strategy for the improvement of fat graft survival with superior angiogenesis

Mou

Peng

et al. 2020

Sci Rep

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platelet-rich plasma (pRp) has been widely used to improve the fat retention rate in autologous fat transplantation since it possesses a good angiogenesis capability in vivo. However, due to the short halflife of growth factors released from PRP and its uneven distribution in injected fat tissue, the strategy of pRp in fat transplantation needs further improvement. Since the capillaries started to grow into fat grafts in 1 week and vascular growth peaks in the second week after transplantation, we hypothesized that delayed two-steps PRP injection into the interior of grafts, accompanied with the extent of neovascularization might theoretically promote microvessel growth inside transplanted adipose tissue. 24 nude mice were divided into three groups: Blank group (0.35 mL fat mixed with 0.15 mL saline, n = 8), Single step group (0.35 mL fat mixed with 0.15 mLPRP, N = 8), and Two steps group (0.35 mL fat (day 0) + 0.075 mL PRP (day 7) + 0.075 mL PRP (day 14), N = 8). At 6 and 14 weeks post-transplantation, grafts were dissected, weighted, and assessed for histology, angiogenesis, fat regeneration and inflammation level. The weight and volume of the fat samples revealed no statistical difference among the three groups at 6 weeks after fat transplantation. The weight and volume of the Two steps group fat samples showed significantly higher compared to that in Blank and Single step groups at 14 weeks after fat transplantation (weight: 137.25 ± 5.60 mg versus 87.5 ± 3.90 mg,106.75 ± 2.94 mg, respectively; volume: 0.13 ± 0.01 mL versus 0.08 ± 0.01 mL, 0.09 ± 0.01 mL, respectively). Histological assessments indicated that delayed two-steps pRp injection strategy helps to improve adipose tissue content and reduce the composition of fibrous connective tissue at 14 weeks after fat transplantation. At 6 weeks and 14 weeks after transplantation, CD31 immunofluorescence indicated that delayed two-steps PRP injection strategy helps to improve angiogenesis and significantly higher compared to that in Blank and Single step groups (6 weeks: 28.75 ± 4.54 versus 10.50 ± 2.06, 21.75 ± 1.85; 14 weeks: 21.75 ± 2.86 versus 9.87 ± 2.08, 11.75 ± 1.47, respectively). Preadipocyte count indicated delayed two-steps PRP injection strategy might promote fat regeneration and significantly higher compared to that in Blank and Single step groups at 14 weeks (129.75 ± 6.57 versus 13.50 ± 3.50, 17.12 ± 6.23, respectively). In this study, we demonstrated that the novel delayed two-steps PRP injection strategy remarkably enhanced the long-term fat retention rate and improved the neovascularization extent in the interior of the fat graft. Platelet-rich plasma, Delayed two-steps injection, Angiogenesis, Fat transplantation Autologous fat transplantation has become popular for its well convenience and biocompatibility in the application of soft-tissue augmentation 1. However, the fat grafting retention rate is still less than ideal 2,3. The results of retention rate are varied (approximately 20% to 75%) 2,3. Many studies have investigated that the adipos...

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“…2, 3, 4 [17]. Luck et al befassten sich in ihrem Review besonders mit den unterschiedlichen angewandten Techniken und konkludierten letztlich, dass die methodische Heterogenität und die zu unpräzise Beschreibung der Techniken in den einzelnen Publikationen eine aussagekräftige Bewertung erschweren [9]. Somit bleibt die Frage nach dem optimalen Protokoll nach wie vor offen und zeigt die Notwendigkeit methodisch korrekter, präzise dokumentierter klinischer Studien.…”

Section: Fallbeispieleunclassified

ACA-Technik: „stromal vascular fraction“, „platelet-rich plasma“ und Mikrofett zur körpereigenen Regeneration und Hautverjüngung

Stevens¹

2018

J Ästhet Chir

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enthält zusätzlich ein Video zur Herstellung von Platelet-Rich-Stroma (PRS oder Autologous Conditioned Plasma Stromal Vascular Fraction [ACP SVF ]). Beitrag und Zusatzmaterial stehen Ihnen im elektronischen Volltextarchiv auf http://www.springermedizin.de/journal-fueraesthetische-chirurgie zur Verfügung. Sie finden das Zusatzmaterial am Beitragsende unter "Supplementary Material". Herstellung von plättchenreichem Plasma und "stromal vascular fraction" at Point-of-Care Plättchenreiches Plasma wird durch Zentrifugation aus einer geringfügigen Menge Eigenblut am Behandlungsort herge

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A Systematic Review of Autologous Platelet-Rich Plasma and Fat Graft Preparation Methods

Abstract: Supplemental Digital Content is available in the text.

Cited by 30 publications

References 77 publications

Autologous fat graft assisted by stromal vascular fraction improves facial skin quality: A randomized controlled trial

Autologous fat graft assisted by stromal vascular fraction improves facial skin quality: A randomized controlled trial

Delayed two steps PRP injection strategy for the improvement of fat graft survival with superior angiogenesis

ACA-Technik: „stromal vascular fraction“, „platelet-rich plasma“ und Mikrofett zur körpereigenen Regeneration und Hautverjüngung

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