A systematic review of quantitative burn wound microbiology in the management of burns patients

Halstead, Fenella D.; Lee, Kwang Chear; Kwei, Johnny; Dretzke, Janine; Oppenheim, Beryl; Moiemen, Naiem

doi:10.1016/j.burns.2017.06.008

Cited by 34 publications

(20 citation statements)

References 53 publications

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“…While swabbing may not be appropriate for absolute quantitation, microbiome sampling by other means (e.g., biopsy 25 ) might be more amenable. However, the relevance of quantitative microbiological data for clinical outcomes, and the techniques for collecting these data, are not yet clear 23 .…”

Section: Discussionmentioning

confidence: 99%

Microbial predictors of healing and short-term effect of debridement on the microbiome of chronic wounds

Verbanic

Shen

Lee

et al. 2020

npj Biofilms Microbiomes

108

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Chronic wounds represent a large and growing disease burden. Infection and biofilm formation are two of the leading impediments of wound healing, suggesting an important role for the microbiome of these wounds. Debridement is a common and effective treatment for chronic wounds. We analyzed the bacterial content of the wound surface from 20 outpatients with chronic wounds before and immediately after debridement, as well as healthy skin. Given the large variation observed among different wounds, we introduce a Bayesian statistical method that models patient-to-patient variability and identify several genera that were significantly enriched in wounds vs. healthy skin. We found no difference between the microbiome of the original wound surface and that exposed by a single episode of sharp debridement, suggesting that this debridement did not directly alter the wound microbiome. However, we found that aerobes and especially facultative anaerobes were significantly associated with wounds that did not heal within 6 months. The facultative anaerobic genus Enterobacter was significantly associated with lack of healing. The results suggest that an abundance of facultative anaerobes is a negative prognostic factor in the chronic wound microbiome, possibly due to the increased robustness of such communities to different metabolic environments.

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Section: Discussionmentioning

confidence: 99%

Microbial predictors of healing and short-term effect of debridement on the microbiome of chronic wounds

Verbanic

Shen

Lee

et al. 2020

npj Biofilms Microbiomes

108

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show abstract

“…If one feels the need to swab the wound, this could be an indication of how worried one may feel about infection, in a wound where infection is not overtly evident. [10] Sometimes a wound does not look infected, but there may be a clinical concern that there is occult infection. In this case, one would do a wound swab.…”

Section: Discussionmentioning

confidence: 99%

A wound assessment and management acronym: BE ME SIC SOS

Kleintjes

2018

S Afr J PRASPB

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Wound assessment comprises the assessment of many factors. The TIME acronym (T= tissue; I = infection/inflammation; M = moisture; E= epithelial edge) has been widely promoted and used for wound assessment. [1] For the purpose of consistent wound assessment for our wound study purposes, there arose a demand for more information to be consistently extracted than that incorporated in TIME. The value of the new acronym is that it provides for consistent wound descriptions in record keeping and of the clinical presentation of wounds, and consistent parameters for research purposes. It is also a useful method for teaching wound assessment to medical students and other personnel.

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“…Two concentrations of bacteria were used for testing according to the "10 5 guideline"; a lower concentration to represent a systemic bacterial load during infection and a higher concentration to represent bacterial load curing cutaneous infection. [24][25][26][27][28]…”

Section: Introductionmentioning

confidence: 99%

In vitro antimicrobial efficacy of two medical grade honey formulations against common high‐risk meticillin‐resistant staphylococci and Pseudomonas spp. pathogens

Cremers¹,

Belas

Costa

et al. 2019

Veterinary Dermatology

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Background Antimicrobial resistance is a problem in human and animal healthcare. Honey may be used for its wound healing properties and antimicrobial effects. Objective To investigate the antimicrobial activity of two commercially available medical grade honeys (MGHs) against Staphylococcus spp. and Pseudomonas spp. isolates. Methods and materials Two formulations, MGH1 (40% w/v honey) and MGH2 (80% w/v Manuka honey), were tested in vitro for minimal inhibitory concentrations (MIC) and minimal bactericidal concentrations (MBC) against 11 Staphylococcus and 11 Pseudomonas isolates at low [1.5 × 104 colony forming units (cfu)/well] and high (1.5 × 106 cfu/well) concentrations of inoculum, representing systemic and cutaneous bacterial loads during infection, respectively. Results MGH2 showed a lower MIC against staphylococci than MGH1, although this was not statistically significant. MGH1 had stronger bactericidal effects against staphylococci than MGH2, although this effect was statistically significant only at the higher bacterial concentration (P < 0.01). For Pseudomonas spp., MGH1 had significantly higher antimicrobial activity (both MIC and MBC) than MGH2 against all isolates tested and at both bacterial concentrations (P < 0.05). Conclusions and clinical importance Both MGHs were effective in vitro against common cutaneous pathogens including meticillin‐resistant staphylococci and Pseudomonas species. The higher efficacy of the MGH1 formulation against Pseudomonas and its consistent effects against staphylococci, while containing only half of the amount of honey compared to MGH2, invites further investigation of the mechanisms and clinical applications of MGH1.

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A systematic review of quantitative burn wound microbiology in the management of burns patients

Abstract: The evidence base for the utility and reliability of quantitative microbiology for diagnosing or predicting clinical outcomes in burns patients is limited and often poorly reported. Consequently future research is warranted.

Cited by 34 publications

References 53 publications

Microbial predictors of healing and short-term effect of debridement on the microbiome of chronic wounds

Microbial predictors of healing and short-term effect of debridement on the microbiome of chronic wounds

A wound assessment and management acronym: BE ME SIC SOS

In vitro antimicrobial efficacy of two medical grade honey formulations against common high‐risk meticillin‐resistant staphylococci and Pseudomonas spp. pathogens

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