A Systematic Review of Stem Cell Differentiation into Keratinocytes for Regenerative Applications

Hazrati, Raheleh; Davaran, Soodabeh; Keyhanvar, Peyman; Soltani, Somaieh; Alizadeh, Effat

doi:10.1007/s12015-023-10636-9

Cited by 3 publications

(2 citation statements)

References 167 publications

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“…On the 14th day, most of the stem cells cultured on the scaffolds were circular/polygonal. On the 21st day and the final evaluation of the cells under the microscope, the formation of multiple clusters was observed (11). Additionally, in the differentiation group with ATRA, presumably due to the presence of a minor amount of DMSO in the culture medium following 4 days of culture on the PD@PCEC@GC hydrogels, (B) following 10 days of culture on the PD@PCEC@GC hydrogels, and (C) following 21 days of cultured on the PD@PCEC@GC hydrogels.…”

Section: Characterization Of Hadscsmentioning

confidence: 99%

“…Meanwhile, stem cells, particularly adipose-derived stem cells (ADSCs), have shown great promise in tissue engineering and regenerative medicine due to their unique properties. − Since ADSCs are easy to access, are abundant, and have multilineage plasticity, they have emerged as a promising skin regeneration candidate . They secrete various growth factors that promote wound healing, making them ideal for enhancing tissue renewal. , Although ADSCs have the potential to differentiate into functional keratinocytes, their efficient and controlled differentiation remains a challenge …”

Section: Introductionmentioning

confidence: 99%

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Development of a Composite Hydrogel Containing Statistically Optimized PDGF-Loaded Polymeric Nanospheres for Skin Regeneration: In Vitro Evaluation and Stem Cell Differentiation Studies

Hazrati,

Alizadeh,

Soltani

et al. 2024

ACS Omega

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Platelet-derived growth factor-BB (PDGF-BB) is a polypeptide growth factor generated by platelet granules faced to cytokines. It plays a role in forming and remodeling various tissue types, including epithelial tissue, through interaction with cell-surface receptors on most mesenchymal origin cells. However, it breaks down quickly in biological fluids, emphasizing the importance of preserving them from biodegradation. To address this challenge, we formulated and evaluated PDGF-encapsulated nanospheres (PD@PCEC) using polycaprolactone-polyethylene glycol-polycaprolactone. PD@PCECs were fabricated through the triple emulsion methodology and optimized by using the Box− Behnken design. The encapsulation efficiency (EE) of nanoencapsulated PDGF-BB was investigated concerning four variables: stirring rate (X1), stirring duration (X2), poly(vinyl alcohol) concentration (X3), and PDGF-BB concentration (X4). The selected optimized nanospheres were integrated into a gelatin-collagen scaffold (PD@PCEC@GC) and assessed for morphology, biocompatibility, in vitro release, and differentiation-inducing activity in human adipose-derived stem cells (hADSCs). The optimized PD@PCEC nanospheres exhibited a particle size of 177.9 ± 91 nm, a zeta potential of 5.2 mV, and an EE of 87.7 ± 0.44%. The release profile demonstrated approximately 85% of loaded PDGF-BB released during the first 360 h, with a sustained release over the entire 504 h period, maintaining bioactivity of 87.3%. The study also included an evaluation of the physicochemical properties of the scaffolds and an assessment of hADSC adhesion to the scaffold's surface. Additionally, hADSCs cultivated within the scaffold effectively differentiated into keratinocyte-like cells (KLCs) over 21 days, evidenced by morphological changes and upregulation of keratinocyte-specific genes, including cytokeratin 18, cytokeratin 19, and involucrin, at both transcriptional and protein levels.

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Section: Characterization Of Hadscsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of a Composite Hydrogel Containing Statistically Optimized PDGF-Loaded Polymeric Nanospheres for Skin Regeneration: In Vitro Evaluation and Stem Cell Differentiation Studies

Hazrati,

Alizadeh,

Soltani

et al. 2024

ACS Omega

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Controlled therapeutic cholesterol delivery to cells for the proliferation and differentiation of keratinocytes

Berniak,

Moradi,

Lichawska-Cieslar

et al. 2024

J. Mater. Chem. B

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The Science of Human Skin

Thanawiroon,

Khumsikiew,

Yingngam

2024

Advances in Chemical and Materials Engineering

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The appearance and health of the skin, the body's largest organ, significantly influence an individual's perceived attractiveness. It appears healthy when it glows and seems aged when it turns rough and becomes susceptible to diseases. For this reason, comprehension of the science behind human skin is the way to create cosmeceuticals that bridge the gap between cosmetics and pharmaceuticals. This chapter aims to provide all the details of the skin's anatomy, its cellular composition by layers, its important processes, and the main external stimuli with which the skin, as a barrier, interacts. The chapter also discusses recent dermatology findings and innovative technologies that have aided in the advancement of cosmeceuticals. To summarize, the above are the key elements of this chapter on the anatomy, physiology, and biological function of the skin.

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A Systematic Review of Stem Cell Differentiation into Keratinocytes for Regenerative Applications

Cited by 3 publications

References 167 publications

Development of a Composite Hydrogel Containing Statistically Optimized PDGF-Loaded Polymeric Nanospheres for Skin Regeneration: In Vitro Evaluation and Stem Cell Differentiation Studies

Development of a Composite Hydrogel Containing Statistically Optimized PDGF-Loaded Polymeric Nanospheres for Skin Regeneration: In Vitro Evaluation and Stem Cell Differentiation Studies

Controlled therapeutic cholesterol delivery to cells for the proliferation and differentiation of keratinocytes

The Science of Human Skin

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