A systematic review of Trichomonas vaginalis in Turkey from 2002 to 2020

Beyhan, Yunus Emre

doi:10.1016/j.actatropica.2021.105995

Cited by 6 publications

(4 citation statements)

References 25 publications

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“…Between 2002 and 2020, 65 articles about T. vaginalis infection in the general Turkish population were found. Infection was found to be prevalent in 5.94% and 6.17% of men and 2.87% of women (20); that was in disagreement with the current study, in which no-significant difference between the rate of infection was 2% for men and 2.04% for women.…”

Section: Discussioncontrasting

confidence: 99%

Evaluation of direct microscopy and culture method for detection of Trichomonas vaginalis in vaginal discharge and urine samples

Abdulhameed Salh,

Mohammed Obaid,

Ali Shafiq

2023

NTU-JPS

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Trichomoniasis has emerged as the most common sexually transmitted disease, and limited data is available on the effective screening technique for the diagnosis of Trichomonas vaginalis (T. vaginalis). This study aimed to compare the ability of two culture media (InPouch TV and Diamond's) to support the growth of clinical isolates of Trichomonas vaginalis and their relative sensitivity for detection of the organism. A total of 343 patients complaining of vaginal discharge of 293 women and urine sample of 50 men were included in the study. From December 2021 to May 2022, from Azadi Teaching Hospital, Private clinics, and Midwifery and Childbearing Hospital in Kirkuk. Three vaginal swabs and a urine sample were screened for trichomoniasis by wet mount microscopy. Diamond Media Culture and Pouch TV were used. The 343 cases studied, 6 women and 1 man were positive by wet mount microscopy, which means 7 (2%) and 336 (98%) were negative. Sterile vaginal swab with centrifugation and urine samples had the highest rate of specificity (85%) to detect TV compared to vaginal swab without centrifugation and Amies Gel Transport Media (AGT) media, which had a lower sensitivity (33% and 16%), respectively. Inpouch TV media was the best culture to grow TV and remained for 10 days, but Local Diamond Modified Media (LDMM) had a poor result for growth of trichomonas vaginalis and only remained alive for 2 days. According to our experiment, the most successful routes of detection are urine and vaginal swab with centrifugation, and Inpouch TV is the unique culture for growth and better than LDMM.

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Section: Discussioncontrasting

confidence: 99%

Evaluation of direct microscopy and culture method for detection of Trichomonas vaginalis in vaginal discharge and urine samples

Abdulhameed Salh,

Mohammed Obaid,

Ali Shafiq

2023

NTU-JPS

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“…Our reported TV prevalence was higher than that reported in Poland (0%), 15 France (1.7%), 16 China (1.64%) 17 and the USA (3.1%), 18 while it was lower than the frequency rates reported in Turkey (6.17%), 19 Brazil (27.8%), 20 Ethiopia (9.62%) 21 and Papua New Guinea (39.3%). 22 Obviously, the higher rates and increased risk of TV in some countries and poor nations may be related to poverty, a low level of education, a lack of knowledge regarding safe sex (e.g.…”

Section: Discussioncontrasting

confidence: 78%

The prevalence ofTrichomonas vaginalisinfection among the female population of Iran: a systematic review and meta-analysis

Moghadamizad

Olfatifar

et al. 2023

International Health

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Trichomonas vaginalis is a protozoan parasite and probably one of the main causes of adverse birth outcomes. Still, there is a paucity of information regarding the burden of T. vaginalis infection in Iranian women. To address this gap, we conducted a systematic review and meta-analysis of studies on trichomoniasis among females in Iran from January 2000 to January 2023. We searched multiple electronic databases (PubMed, Science Direct, Scopus, ProQuest, Web of Science, Google Scholar, Scientific Information Database, Iran Medex, Magiran and Iran Doc) and identified 108 articles with a total of 200 728 women. Using a random effects model, we found a pooled prevalence of 4.30% (95% CI 3.23 to 5.52%). Subgroup analysis, also, demonstrated a statistically significant association between the T. vaginalis prevalence in Iranian women, and the age range, city, sample type, being in prison, sex working and symptomatic infections. Our findings provide important information for healthcare authorities and can inform prevention strategies against trichomoniasis in Iran.

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“…According to the latest estimates, the annual incidence of T. vaginalis worldwide is higher than that of chlamydia and gonorrhea combined (Bouchemal et al, 2017). From 2002 to 2020, the T. vaginalis prevalence in Turkey was 5.94% (2.87% in men and 6.17% in women; Beyhan, 2021). According to the results of a cross‐sectional study in Fujian and Sichuan Provinces, China, the prevalences of T. vaginalis were 3.2% and 0.7% (Chen et al, 2006; Yongjun et al, 2009), respectively.…”

Section: Discussionmentioning

confidence: 99%

Construction a novel detection method for Trichomonas vaginalis based on recombinant enzyme polymerase amplification targeting the Actin gene

Deng

Sheng

et al. 2023

J Eukaryotic Microbiology

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Trichomoniasis is a common and curable sexually transmitted disease worldwide. The rapid, convenient, and accurate diagnosis of trichomoniasis is an important link in the prevention and treatment of the disease. The current detection methods of Trichomonas vaginalis are mainly wet mount microscopy, culture, nested PCR, and loop‐mediated isothermal amplification. However, these detection methods have some shortcomings. In this study, a recombinant enzyme polymerase amplification (RPA) assay had been conducted to detect T. vaginalis. The target gene and the corresponding primers were screened, and the reaction system and conditions were optimized in the assay of RPA. The sensitivity and specificity of this detection method were analyzed. The detection efficiency of wet mount microscopy, culture, nested PCR, and RPA was compared by testing 53 clinical samples from vaginal secretions. By screening, the actin gene of T. vaginalis could be used as a target gene for RPA detection of T. vaginalis, and the optimum reaction condition to amplify the actin gene by RPA was at 39°C for 30 min. The detection limit of T. vaginalis DNA using RPA was 1 pg, corresponding to a sensitivity of approximately five trophozoites. The RPA assay demonstrated high specificity for T. vaginalis, and there was no cross‐reactivity with Giardia lamblia, Escherichia coli, Lactobacillus, Toxoplasma gondii, Staphylococcus aureus, and Candida albicans. Of the 53 clinical samples, the positive rates of T. vaginalis detected by wet mount microscopy, culture, nested PCR and RPA were 50.9 4% (27/53), 71.7% (38/53), 71.7% (38/53), and 69.81% (37/53), respectively. Compared with culture which was used as the gold standard for diagnosing trichomoniasis, testing clinical samples by wet mount microscopy showed 71.05% sensitivity, 100% specificity, and moderate diagnostic agreement with the culture (K = 0.581, Z = 4.661, p < 0.001). The nested PCR showed 100% sensitivity, 100% specificity, and excellent diagnostic agreement (K = 1, Z = 7.28, p < 0.001), while RPA displayed 97.37% sensitivity, 100% specificity, and excellent diagnostic agreement (K = 0.954, Z = 6.956, p < 0.001). At the present study, rapid amplification of actin gene by RPA could be used as a tool for detection of T. vaginalis. The detection method of RPA was more sensitive than wet mount microscopy and displayed excellent specificity. Moreover, RPA amplification of actin gene did not require a PCR instrument and the amplification time was shorter than that of ordinary PCR. Therefore, the RPA assay was proposed in this study as a point‐of‐care examination and a diagnostic method of T. vaginalis infection, which exhibited the potential value in the treatment and prevention of trichomoniasis.

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A systematic review of Trichomonas vaginalis in Turkey from 2002 to 2020

Cited by 6 publications

References 25 publications

Evaluation of direct microscopy and culture method for detection of Trichomonas vaginalis in vaginal discharge and urine samples

Evaluation of direct microscopy and culture method for detection of Trichomonas vaginalis in vaginal discharge and urine samples

The prevalence ofTrichomonas vaginalisinfection among the female population of Iran: a systematic review and meta-analysis

Construction a novel detection method for Trichomonas vaginalis based on recombinant enzyme polymerase amplification targeting the Actin gene

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