A Systematic Survey of Characteristic Features of Yeast Cell Death Triggered by External Factors

Grosfeld, Erika V.; Bidiuk, Victoria; Mitkevich, Olga V.; Kushnirov, Vitaly V.; Alexandrov, Alexander I.

doi:10.3390/jof7110886

Cited by 19 publications

(16 citation statements)

References 190 publications

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“…There are also numerous cases of apoptosis-like cell death in yeast where a shock causes cells to cease division but not exhibit immediate membrane permeabilization. A simple experimental indicator of this type of cell death is a non-congruence between the number of PI-positive cells and non-viable cell numbers as determined by colony-forming assays [ 29 , 30 ].…”

Section: Resultsmentioning

confidence: 99%

Theoretical and Experimental Assay of Shock Experienced by Yeast Cells during Laser Bioprinting

Grosfeld

Zhigarkov

Alexandrov

et al. 2022

IJMS

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Laser-induced forward transfer (LIFT) is a useful technique for bioprinting using gel-embedded cells. However, little is known about the stresses experienced by cells during LIFT. This paper theoretically and experimentally explores the levels of laser pulse irradiation and pulsed heating experienced by yeast cells during LIFT. It has been found that only 5% of the cells in the gel layer adjacent to the absorbing Ti film should be significantly heated for fractions of microseconds, which was confirmed by the fact that a corresponding population of cells died during LIFT. This was accompanied by the near-complete dimming of intracellular green fluorescent protein, also observed in response to heat shock. It is shown that microorganisms in the gel layer experience laser irradiation with an energy density of ~0.1–6 J/cm2. This level of irradiation had no effect on yeast on its own. We conclude that in a wide range of laser fluences, bioprinting kills only a minority of the cell population. Importantly, we detected a previously unobserved change in membrane permeability in viable cells. Our data provide a wider perspective on the effects of LIFT-based bioprinting on living organisms and might provide new uses for the procedure based on its effects on cell permeability.

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Section: Resultsmentioning

confidence: 99%

Theoretical and Experimental Assay of Shock Experienced by Yeast Cells during Laser Bioprinting

Grosfeld

Zhigarkov

Alexandrov

et al. 2022

IJMS

Self Cite

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show abstract

“…With respect to its progeny, S. cerevisiae YVGC13A reacted to copper’s supplementation by inducing genes responsible for carbohydrates metabolism and protein biosynthesis as a general mechanism of stress response in this species ( Figure 5 E,F) [ 45 , 49 ]. Conversely, the higher intensities in fatty acids may be the result of higher reactive oxygen species (ROS) accumulation, already described as a specific S. cerevisiae response to stress conditions triggered by copper [ 50 , 51 ].…”

Section: Resultsmentioning

confidence: 99%

Renewing Lost Genetic Variability with a Classical Yeast Genetics Approach

Gupte

Pierantoni

Conti

et al. 2023

JoF

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Due to their long domestication time course, many industrial Saccharomyces cerevisiae strains are adopted in numerous processes mostly for historical reasons instead of scientific and technological needs. As such, there is still significant room for improvement for industrial yeast strains relying on yeast biodiversity. This paper strives to regenerate biodiversity with the innovative application of classic genetic methods to already available yeast strains. Extensive sporulation was indeed applied to three different yeast strains, specifically selected for their different origins as well as backgrounds, with the aim of clarifying how new variability was generated. A novel and easy method to obtain mono-spore colonies was specifically developed, and, to reveal the extent of the generated variability, no selection after sporulation was introduced. The obtained progenies were then tested for their growth in defined mediums with high stressor levels. A considerable and strain-specific increase in both phenotypic and metabolomic variability was assessed, and a few mono-spore colonies were found to be of great interest for their future exploitation in selected industrial processes.

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“…(D) Under the “Dex-trade-off” condition, azf1 Δ cells were more severely stained by Phloxine B (pink color). Phloxine B is a negatively-charged dye that only stains cells intracellularly when cell membrane intactness has been compromised (Grosfeld et al 2021). (E) The azf1 Δ mutant is more sensitive to mild cell wall disruption.…”

Section: Resultsmentioning

confidence: 99%

Rapid compensatory evolution by secondary perturbation of a primary disrupted transcriptional network

Hsu

Cheng

Liao

et al. 2022

Preprint

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The discrete steps of transcriptional rewiring have been proposed to occur neutrally to ensure steady gene expression under stabilizing selection over long time-scales, especially when a regulon is being transferred from one transcription factor (TF) to another. Cooperative DNA binding between redundant regulatory components at the intermediate transition stage is believed to mediate this process, enabling a conflict-free switch between two TFs without a disruptive change in gene expression. Here, we have performed an evolutionary repair experiment on the Lachancea kluyveri yeast sef1Δ mutant by means of a suppressor development strategy. Complete loss of SEF1 forced cells to activate a rewiring process to compensate for the pleiotropic defects arising from misexpression of multiple TCA cycle genes. Using different selective conditions, we identified one generalist and one specialist suppressive loss-of-function mutation of IRA1 and AZF1, respectively. Our subsequent analyses show that Azf1 is a weak transcriptional activator regulated by the Ras1-PKA pathway. Azf1 loss-of-function triggers extensive gene expression changes responsible for both the compensatory and trade-off phenotypes. Our results indicate that the pleiotropic effects of dual perturbation of transcriptional networks are a potential mechanism for rapid adaptive compensation, facilitating the process of incipient transcriptional rewiring, and formation of complex traits.

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A Systematic Survey of Characteristic Features of Yeast Cell Death Triggered by External Factors

Cited by 19 publications

References 190 publications

Theoretical and Experimental Assay of Shock Experienced by Yeast Cells during Laser Bioprinting

Theoretical and Experimental Assay of Shock Experienced by Yeast Cells during Laser Bioprinting

Renewing Lost Genetic Variability with a Classical Yeast Genetics Approach

Rapid compensatory evolution by secondary perturbation of a primary disrupted transcriptional network

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