A Systems Approach to Uncover the Effects of the PGPR Pseudomonas koreensis on the Level of Drought Stress Tolerance in Helianthus Annuus

Macleod, Kyle; Rumbold, Karl; Padayachee, Kershree

doi:10.1016/j.proenv.2015.07.200

Cited by 10 publications

(3 citation statements)

References 2 publications

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“…capsici ) [ 58 ], and two siderophore-producing strains of B. amyloliquefaciens eliminated Ralstonia solanacearum -induced bacterial wilt in tomatoes [ 59 ]. Likewise, increased siderophore production at even higher levels of water stress has been reported [ 60 , 61 ].…”

Section: Resultsmentioning

confidence: 93%

Drought Tolerant Enterobacter sp./Leclercia adecarboxylata Secretes Indole-3-acetic Acid and Other Biomolecules and Enhances the Biological Attributes of Vigna radiata (L.) R. Wilczek in Water Deficit Conditions

Ahmed

Shahid

Syed

et al. 2021

Biology

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Drought or water stress is a limiting factor that hampers the growth and yield of edible crops. Drought-tolerant plant growth-promoting rhizobacteria (PGPR) can mitigate water stress in crops by synthesizing multiple bioactive molecules. Here, strain PAB19 recovered from rhizospheric soil was biochemically and molecularly characterized, and identified as Enterobacter sp./Leclercia adecarboxylata (MT672579.1). Strain PAB19 tolerated an exceptionally high level of drought (18% PEG-6000) and produced indole-3-acetic acid (176.2 ± 5.6 µg mL−1), ACC deaminase (56.6 ± 5.0 µg mL−1), salicylic acid (42.5 ± 3.0 µg mL−1), 2,3-dihydroxy benzoic acid (DHBA) (44.3 ± 2.3 µg mL−1), exopolysaccharide (204 ± 14.7 µg mL−1), alginate (82.3 ± 6.5 µg mL−1), and solubilized tricalcium phosphate (98.3 ± 3.5 µg mL−1), in the presence of 15% polyethylene glycol. Furthermore, strain PAB19 alleviated water stress and significantly (p ≤ 0.05) improved the overall growth and biochemical attributes of Vigna radiata (L.) R. Wilczek. For instance, at 2% PEG stress, PAB19 inoculation maximally increased germination, root dry biomass, leaf carotenoid content, nodule biomass, leghaemoglobin (LHb) content, leaf water potential (ΨL), membrane stability index (MSI), and pod yield by 10%, 7%, 14%, 38%, 9%, 17%, 11%, and 11%, respectively, over un-inoculated plants. Additionally, PAB19 inoculation reduced two stressor metabolites, proline and malondialdehyde, and antioxidant enzymes (POD, SOD, CAT, and GR) levels in V. radiata foliage in water stress conditions. Following inoculation of strain PAB19 with 15% PEG in soil, stomatal conductance, intercellular CO2 concentration, transpiration rate, water vapor deficit, intrinsic water use efficiency, and photosynthetic rate were significantly improved by 12%, 8%, 42%, 10%, 9% and 16%, respectively. Rhizospheric CFU counts of PAB19 were 2.33 and 2.11 log CFU g−1 after treatment with 15% PEG solution and 8.46 and 6.67 log CFU g−1 for untreated controls at 40 and 80 DAS, respectively. Conclusively, this study suggests the potential of Enterobacter sp./L. adecarboxylata PAB19 to alleviate water stress by improving the biological and biochemical features and of V. radiata under water-deficit conditions.

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Section: Resultsmentioning

confidence: 93%

Drought Tolerant Enterobacter sp./Leclercia adecarboxylata Secretes Indole-3-acetic Acid and Other Biomolecules and Enhances the Biological Attributes of Vigna radiata (L.) R. Wilczek in Water Deficit Conditions

Ahmed

Shahid

Syed

et al. 2021

Biology

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“…For instance, Ródenas et al found that P. mandelii strain 29 could signi cantly increase mycorrhizal colonization (Navarro-Ródenas et al 2016), while another study found that P. mandelii was associated with rice plant nitrogen xation. Pseudomonas koreensis has not been reported on much, though P. koreensis Ps 9-14T has been isolated from the soil (Kwon et al 2003), and another study discovered that P. koreensis could enhance the drought resistance of Helianthus annuus (Macleod et al 2015). A P. koreensis JDM-2 strain was isolated from Eucommia ulmoides roots and exhibited ACC deaminase activity, and found that it had signi cant antibacterial effect on Bacillus subtilis (Gong 2011).…”

Section: Discussionmentioning

confidence: 99%

Effects of Plant Growth Promoting Rhizobacteria Microbial Inoculants on the Growth, Rhizosphere Soil Properties, and Bacterial Community of Pinus sylvestris var. mongolica Annual Seedlings

Song

Deng

et al. 2020

Preprint

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[Objective] Determine the ability of three plant growth promoting rhizobacteria (PGPR) strains (Pseudomonas Mandelli A36, Serratia plymuthica A13 and Pseudomonas koreensis A20) to promote plant growth, evaluate the effect of inoculation with PGPR strains on seedling biomass, root structure, nutrient index, and enzyme activity, and assess the effect of PGPR inoculation on soil nutrient index, enzyme activity, and the soil microecological environment.[Method] The ability of the three PGPR strains to secrete indole-3-acetic acid (IAA), dissolve inorganic phosphorus, and produce siderophore and hydrolase was determined by the medium color change method, pot experiment to determine the effects of three PGPR strains on plant biomass, physicochemical properties, soil physicochemical properties and microbial diversity. [Result] The three PGPR strains had the ability to secrete IAA, solubilize inorganic phosphorus, and produce siderophore, the results of the pot experiment showed that inoculation with PGPR strain had a significant effect on plant biomass, root index, nutrient index and enzyme activity, as well as soil nutrient index, enzyme activity and bacterial diversity. [Conclusion] This study provides basic data references for PGPR strains to improve the soil microecological environment and promote the growth and development of Pinus sylvestris var. Mongolica seedlings.

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“…Under these unsettled conditions, the abundance of microbial generalists in the soil might be promoted, which can cope with a broad range of resources, and the abundance of specialists might be reduced [ 21 ]. For pseudomonads, the P. putida and P. koreensis strains, which have previously been reported as common members of the plant growth-promoting and drought-tolerant Pseudomonas community [ 22 , 23 ], are expected to dominate the Pseudomonas community. In this respect, the mediating role of plant community composition on the impact of climate change on soil pseudomonades, such as important plant growth-promoting soil bacteria [ 24 , 25 ], is of particular interest.…”

Section: Introductionmentioning

confidence: 99%

Drought and Plant Community Composition Affect the Metabolic and Genotypic Diversity of Pseudomonas Strains in Grassland Soils

et al. 2021

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Climate and plant community composition (PCC) modulate the structure and function of microbial communities. In order to characterize how the functional traits of bacteria are affected, important plant growth-promoting rhizobacteria of grassland soil communities, pseudomonads, were isolated from a grassland experiment and phylogenetically and functionally characterized. The Miniplot experiment was implemented to examine the mechanisms underlying grassland ecosystem changes due to climate change, and it investigates the sole or combined impact of drought and PCC (plant species with their main distribution either in SW or NE Europe, and a mixture of these species). We observed that the proportion and phylogenetic composition of nutrient-releasing populations of the Pseudomonas community are affected by prolonged drought periods, and to a minor extent by changes in plant community composition, and that these changes underlie seasonality effects. Our data also partly showed concordance between the metabolic activities and 16S phylogeny. The drought-induced shifts in functional Pseudomonas community traits, phosphate and potassium solubilization and siderophore production did not follow a unique pattern. Whereas decreased soil moisture induced a highly active phosphate-solubilizing community, the siderophore-producing community showed the opposite response. In spite of this, no effect on potassium solubilization was detected. These results suggest that the Pseudomonas community quickly responds to drought in terms of structure and function, the direction of the functional response is trait-specific, and the extent of the response is affected by plant community composition.

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A Systems Approach to Uncover the Effects of the PGPR Pseudomonas koreensis on the Level of Drought Stress Tolerance in Helianthus Annuus

Cited by 10 publications

References 2 publications

Drought Tolerant Enterobacter sp./Leclercia adecarboxylata Secretes Indole-3-acetic Acid and Other Biomolecules and Enhances the Biological Attributes of Vigna radiata (L.) R. Wilczek in Water Deficit Conditions

Drought Tolerant Enterobacter sp./Leclercia adecarboxylata Secretes Indole-3-acetic Acid and Other Biomolecules and Enhances the Biological Attributes of Vigna radiata (L.) R. Wilczek in Water Deficit Conditions

Effects of Plant Growth Promoting Rhizobacteria Microbial Inoculants on the Growth, Rhizosphere Soil Properties, and Bacterial Community of Pinus sylvestris var. mongolica Annual Seedlings

Drought and Plant Community Composition Affect the Metabolic and Genotypic Diversity of Pseudomonas Strains in Grassland Soils

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