A TA/Cu2+ Nanoparticle Enhanced Carboxymethyl Chitosan-Based Hydrogel Dressing with Antioxidant Properties and Promoting Wound Healing

Abstract

Background As the first line of immune defense and the largest organ of body, skin is vulnerable to damage caused by surgery, burns, collisions and other factors. Wound healing in the skin is a long and complex physiological process that is influenced by a number of different factors. Proper wound care can greatly improve the speed of wound healing and reduce the generation of scars. However, traditional wound dressings (bandages, gauze, etc.) often used in clinical practice have a single function, lack of active ingredients and are limited in use. Hydrogels with three-dimensional network structure are a potential biomedical material because of their physical and chemical environment similar to extracellular matrix. In particular, hydrogel dressings with low price, good biocompatibility, degradability, antibacterial and angiogenic activity are favored by the public. Methods Here, a carboxymethyl chitosan-based hydrogel dressing (CMCS-TA/Cu 2+ ) reinforced by copper ion crosslinked tannic acid (TA/Cu 2+ ) nanoparticles was developed. This study investigated the physical and chemical characteristics, cytotoxicity, and angiogenesis of TA/Cu 2+ nanoparticles and CMCS-TA/Cu 2+ hydrogels. Furthermore, a full-thickness skin defect wound model was employed to assess the in vivo wound healing capacity of hydrogel dressings. Results The introduction of TA/Cu 2+ nanoparticles not only could increase the mechanical properties of the hydrogel but also continuously releases copper ions to promote cell migration (the cell migration could reach 92% at 48 h) and tubule formation, remove free radicals and promote wound healing (repair rate could reach 90% at 9 days). Conclusion Experiments have proved that CMCS-TA/Cu 2+ hydrogel has good cytocompatibility, antioxidant and wound healing ability, providing an advantageous solution for skin repair.

Cu2O Nanocubes Embedded in Polycaprolactone Nanofibers for Photo–chemotherapeutic Wound Disinfection and Regeneration

“…The appropriate Cu 2+ release can inhibit bacteria to a certain extent, even without light irradiation, thereby help reduce bacterial contamination during material storage and surgery . In addition, the slow release rate confined the concentration of Cu 2+ within an appropriate range that can avoid the dose-dependent toxicity. , Meanwhile, the Cu 2+ were given in doses that can promote cell migration and thus accelerate wound healing . Over the course of 14 days, the total release reached about 20 μg, equivalent to a daily release of about 1.43 μg, far less than the maximum daily intake of copper (ca.…”

“…50,51 Meanwhile, the Cu 2+ were given in doses that can promote cell migration and thus accelerate wound healing. 52 Over the course of 14 days, the total release reached about 20 μg, equivalent to a daily release of about 1.43 μg, far less than the maximum daily intake of copper (ca. 10 mg/d).…”

Antioxidative, Anti-Inflammatory, Antibacterial, Photo-Cross-Linkable Hydrogel of Gallic Acid–Chitosan Methacrylate: Synthesis, In Vitro, and In Vivo Assessments

“…Similar to many other commonly used natural polymers, such as gelatin, collagen, and hyaluronic acid, CS is cost-effective with good biocompatibility and unique antibacterial activity, making it attractive for a wide range of applications in the biomedical field. Especially, CS-based hydrogels have gained increasing attention for drug delivery, wound healing, health monitoring, and bioadhesives …”