A targeted analysis of flavonoids in asparagus using the UPLC-MS technique

Abstract

Production development for asparagus has become an important research subject due to its low shelf life. In order to determine the content of flavonoids in asparagus tips and shoots, LC-MS-based method was performed for a targeted analysis of flavonoids in asparagus, and 34 peaks attributed to the targeted flavonoids were characterised. Twelve peaks corresponding to rutin, isoquercitrin, quercetin, naringin, taxifolin, vitexin, genistin, daidzein, luteolin, chrysin, and kaempferide were identified and quantified from the asparagus tips and shoots by the LC-MS-based detection with monitoring of parent/daughter ions. The results showed that rutin (> 99%) was the main flavonoid present in the asparagus tips and shoots. Although the tips and shoots contained almost similar compounds, the content of the major compounds, especially rutin, was significantly different. Therefore, the method established through this study could be used for quantitative analysis of flavonoids, especially rutins, in asparagus. The result will provide a theoretical basis for food development in asparagus.<br /><br />

Nutricosmetic effects of Asparagus officinalis: a potent matrix metalloproteinase-1 inhibitor

“…A. officinalis tips contained a significantly higher gallic acid and rutin content than the spear ( p < 0.05). The results were in accordance with previous studies reporting that the A. officinalis tip was a rich source of gallic acid and rutin 18 , 19 . In addition, the present study revealed that both drying temperature and drying duration significantly affected the gallic acid and rutin content in both A. officinalis tip and spear.…”

“…Since rutin was reported as the main flavonoid present in the tip and spear of A. officinalis 18 , the content of rutin was also investigated in the present study. HPLC chromatograms of standard gallic acid, standard rutin, and A. officinalis extracts are shown in Fig.…”

Phytochemical Profiling by UPLC‐ESI‐QTOF‐MS of Kalaharia uncinata (Schinz) Moldenke, Widely Used in Traditional Medicine in DR Congo

“…They exhibited similar molecular ions at m/z 299.0556 [M−H] − and m/z 301.0712 [M+H] + , suggesting the molecular formula C 16 H 12 O 6 . The mass spectra of both compounds 20 and 23 observed in the AcOEt and the MeOH 90 % fractions, respectively, exhibited in ESI − mode an abundant fragment ion at m/z 284 [M−H‐15] − , after the loss of 15 Da (most probably a methyl group) and fit with many authors for kaempferide [46–49] and Zhang et al [45] . for diosmetin.…”

“…Compound 1, which are consistent with Gu et al [63] for methyl-quercetin. Based on LC/MS data (Table 1 , after the loss of 15 Da (most probably a methyl group) and fit with many authors for kaempferide [46][47][48][49] and Zhang et al [45] for diosmetin. Thus, based on LC/MS data reported in Table 1, compounds 20 and 23 are most probably trihydroxy-methoxyflavones.…”

Development and application of mass spectrometric molecular networking for analyzing the ingredients of areca nut

“…In positive mode, the network consisted of 7115 nodes and 7455 edges, while in By comparing the MS-DIAL metabolic database and GNPS annotation results, we determined the chemical information of the nodes in the molecular network, marking different compound categories with distinct colors. In total, we annotated 17 phenols, 43,44 6 alkaloids, 45 4 carbohydrates, 46 5 amino acids, 10 lipids, 47 5 nucleosides and 1 organic acid within the network.…”