A targeted metabolomic procedure for amino acid analysis in different biological specimens by ultra-high-performance liquid chromatography–tandem mass spectrometry

Casado, Mercedes; Sierra, Cristina; Batllori, Marta; Artuch, Rafael; Ormazábal, Aída

doi:10.1007/s11306-018-1374-4

Cited by 37 publications

(25 citation statements)

References 32 publications

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“…After 10-minute incubation on ice, proteins were pelleted at 13,000 rpm for 5 minutes. Hippocampal amino acids were analyzed by ultra-high-performance liquid chromatography (UPLC) coupled to MS/MS, as previously reported [ 99 ]. Briefly, amino acids were separated in a Waters ACQUITY UPLC H-class chromatograph and quantified with a Waters Xevo TQD triple-quadrupole mass spectrometer using positive electrospray ionization conditions in the MRM mode.…”

Section: Methodsmentioning

confidence: 99%

Generation of mitochondrial reactive oxygen species is controlled by ATPase inhibitory factor 1 and regulates cognition

et al. 2021

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The mitochondrial ATP synthase emerges as key hub of cellular functions controlling the production of ATP, cellular signaling, and fate. It is regulated by the ATPase inhibitory factor 1 (IF1), which is highly abundant in neurons. Herein, we ablated or overexpressed IF1 in mouse neurons to show that IF1 dose defines the fraction of active/inactive enzyme in vivo, thereby controlling mitochondrial function and the production of mitochondrial reactive oxygen species (mtROS). Transcriptomic, proteomic, and metabolomic analyses indicate that IF1 dose regulates mitochondrial metabolism, synaptic function, and cognition. Ablation of IF1 impairs memory, whereas synaptic transmission and learning are enhanced by IF1 overexpression. Mechanistically, quenching the IF1-mediated increase in mtROS production in mice overexpressing IF1 reduces the increased synaptic transmission and obliterates the learning advantage afforded by the higher IF1 content. Overall, IF1 plays a key role in neuronal function by regulating the fraction of ATP synthase responsible for mitohormetic mtROS signaling.

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Section: Methodsmentioning

confidence: 99%

Generation of mitochondrial reactive oxygen species is controlled by ATPase inhibitory factor 1 and regulates cognition

et al. 2021

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“…To increase sensitivity and enhanced chromatographic separation, derivatization of dipeptides with 6-aminoquinolyl-N-hydroxysuccinimudyl carbamate (AQC), also known under the brand name AccQ-Tag™ (Waters), was applied. AQC labels primary as well as secondary amines and is widely used for amino acid analysis [ 10 , 11 , 12 ]. Via this method 36 dipeptides could be identified and quantified.…”

Section: Introductionmentioning

confidence: 99%

A Novel UPLC-MS/MS Method Identifies Organ-Specific Dipeptide Profiles

Heidenreich

Pfeffer

Kracke

et al. 2021

IJMS

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Background: Amino acids have a central role in cell metabolism, and intracellular changes contribute to the pathogenesis of various diseases, while the role and specific organ distribution of dipeptides is largely unknown. Method: We established a sensitive, rapid and reliable UPLC-MS/MS method for quantification of 36 dipeptides. Dipeptide patterns were analyzed in brown and white adipose tissues, brain, eye, heart, kidney, liver, lung, muscle, sciatic nerve, pancreas, spleen and thymus, serum and urine of C57BL/6N wildtype mice and related to the corresponding amino acid profiles. Results: A total of 30 out of the 36 investigated dipeptides were detected with organ-specific distribution patterns. Carnosine and anserine were most abundant in all organs, with the highest concentrations in muscles. In liver, Asp-Gln and Ala-Gln concentrations were high, in the spleen and thymus, Glu-Ser and Gly-Asp. In serum, dipeptide concentrations were several magnitudes lower than in organ tissues. In all organs, dipeptides with C-terminal proline (Gly-Pro and Leu-Pro) were present at higher concentrations than dipeptides with N-terminal proline (Pro-Gly and Pro-Leu). Organ-specific amino acid profiles were related to the dipeptide profile with several amino acid concentrations being related to the isomeric form of the dipeptides. Aspartate, histidine, proline and serine tissue concentrations correlated with dipeptide concentrations, when the amino acids were present at the C- but not at the N-terminus. Conclusion: Our multi-dipeptide quantification approach demonstrates organ-specific dipeptide distribution. This method allows us to understand more about the dipeptide metabolism in disease or in healthy state.

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“…Since then, several compilations of reference ranges for urine metabolites have been published, with a very good book being published by N. Blau et al [8]; and there are extensive Internet resources which are also worth being mentioned [48][49][50]. Valuable reference concentrations in urine for infants and children [51,52], and neonates and infants [24,51,53], have been recently published. In most cases ranges of normal concentrations previously published represent compilations of single samples (one shot) from a large number of control cases.…”

Section: Resultsmentioning

confidence: 99%

Monitoring Methylmalonic Aciduria by NMR Urinomics

Nicolescu

Daniela²,

Boiciuc³

et al. 2020

Molecules

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The paper reports on monitoring methylmalonic aciduria (MMA)-specific and non-specific metabolites via NMR urinomics. Five patients have been monitored over periods of time; things involved were diet, medication and occasional episodes of failing to comply with prescribed diets. An extended dataset of targeted metabolites is presented, and correlations with the type of MMA are underlined. A survey of previous NMR studies on MMA is also presented.

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A targeted metabolomic procedure for amino acid analysis in different biological specimens by ultra-high-performance liquid chromatography–tandem mass spectrometry

Cited by 37 publications

References 32 publications

Generation of mitochondrial reactive oxygen species is controlled by ATPase inhibitory factor 1 and regulates cognition

Generation of mitochondrial reactive oxygen species is controlled by ATPase inhibitory factor 1 and regulates cognition

A Novel UPLC-MS/MS Method Identifies Organ-Specific Dipeptide Profiles

Monitoring Methylmalonic Aciduria by NMR Urinomics

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