A technique for correction of attenuations in synchronous fluorescence spectroscopy

Devi, Sobha Lalitha; Ghosh, Nirmalya; Pradhan, Asima

doi:10.1016/j.jphotobiol.2015.06.019

Cited by 9 publications

(1 citation statement)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To reduce the absorption effects in the output polarized spectra, a ratio is taken between the resultant fluorescence and elastic scattering spectra. This intrinsic fluorescence extraction scheme has been experimentally validated by several groups on tissue mimicking phantoms and in-vitro cervical tissue samples 17 , 35 , 40 – 42 .…”

Section: Methodsmentioning

confidence: 99%

Design, fabrication and testing of 3D printed smartphone-based device for collection of intrinsic fluorescence from human cervix

Shukla

Sah

Hatiboruah

et al. 2022

Sci Rep

Self Cite

View full text Add to dashboard Cite

Fluorescence spectroscopy has the potential to identify discriminatory signatures, crucial for early diagnosis of cervical cancer. We demonstrate here the design, fabrication and testing of a 3D printed smartphone based spectroscopic device. Polarized fluorescence and elastic scattering spectra are captured through the device using a 405 nm laser and a white LED source respectively. The device has been calibrated by comparison of spectra of standard fluorophores (Flavin adenine dinucleotide, fluorescein, rhodamine, and porphyrin) with the corresponding spectra collected from a commercial spectrometer. A few cervical tissue spectra have also been captured for proof of its applicability as a portable, standalone device for the collection of intrinsic fluorescence spectra from human cervix.

show abstract

Section: Methodsmentioning

confidence: 99%

Design, fabrication and testing of 3D printed smartphone-based device for collection of intrinsic fluorescence from human cervix

Shukla

Sah

Hatiboruah

et al. 2022

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

Human Saliva for Oral Precancer Detection: a Comparison of Fluorescence & Stokes Shift Spectroscopy

et al. 2017

View full text Add to dashboard Cite

We report here a study on human saliva tested as a diagnostic medium for oral cancer detection on three groups: oral squamous cell carcinoma (OSCC), oral sub mucous fibrosis (OSMF; precancer), and healthy controls (normal). Measurements have been conducted using fluorescence spectroscopy with 350 nm excitation and Stokes shift (SS) spectroscopy (SSS) with 120 nm shift from a total of 99 saliva samples. For classification, principal component analysis (PCA) and linear discriminant analysis (LDA) have been applied on the data sets. Linear discriminant (LD) scores of fluorescence spectra are able to differentiate OSCC to normal, OSMF to normal and OSCC to OSMF with sensitivities 91%, 92%, 91% and specificities 97%, 100%, 94% respectively, while LD scores of SS spectra differentiate respective groups with sensitivities 100%, 94%, 94% and specificities 97%, 100%, 94%. Cross-validation on the datasets of PC scores during LDA illustrates that sensitivity and specificity of SSS data are less affected than those of fluorescence data. Saliva is thus seen as a potential non-invasive and simple diagnostic medium, with SS spectroscopy as a better diagnostic tool for oral precancer.

show abstract

Synchronous luminescence spectroscopy of tryptophan in head and neck cancer

Ganesan

Gnanatheepam

Aruna

2022

Biophotonics, Tryptophan and Disease

View full text Add to dashboard Cite

A technique for correction of attenuations in synchronous fluorescence spectroscopy

Cited by 9 publications

References 37 publications

Design, fabrication and testing of 3D printed smartphone-based device for collection of intrinsic fluorescence from human cervix

Design, fabrication and testing of 3D printed smartphone-based device for collection of intrinsic fluorescence from human cervix

Human Saliva for Oral Precancer Detection: a Comparison of Fluorescence & Stokes Shift Spectroscopy

Synchronous luminescence spectroscopy of tryptophan in head and neck cancer

Contact Info

Product

Resources

About