A Technique for Heart Transplantation In the Rat

Abbott, Charles P.; Lindsey, Edward S.; Creech, Oscar; Dewitt, Charles W.

doi:10.1001/archsurg.1964.01320040061009

Cited by 114 publications

(47 citation statements)

References 5 publications

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“…At this time, the need has been addressed by using heterotopic, nonloaded heart grafts transplanted to the aAo of rodents [3,4,5,6]. Such a retrogradely perfused and vascularized heart, even though allowing organ transplantation research, cannot be used to address questions related to full native-analogous myocardial functionality and metabolic activity.…”

Section: Discussionmentioning

confidence: 99%

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Hemodynamic Assessment of a Murine Heterotopic Biventricularly Loaded Cardiac Transplant in vivo Model

et al. 2016

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Background: Heterotopic heart transplantation (HHT) in rodent animal models represents an important technique enabling studies on organ transplantation immunology and pharmaceutical development. Recent investigations used nonworking HHT designs, with the left ventricle (LV) bypassed in the anastomosis system. In spite of their principal success, the lack of orthogonal ventricular filling leads to myocardial atrophy. However, when focusing on the cellular and molecular mechanisms involved in the in vivo remodeling of the myocardium or cell-based cardiovascular implants, a nonworking model is suboptimal as it lacks the native-analogous hemodynamic and metabolic situation. Here we present the hemodynamic and electrical assessment of a biventricularly loaded murine HHT method without the need for a combined heart-lung transplantation approach. Methods: Heterotopic transplantations (n = 13) were performed on C57BL/6J-(H-2b) inbred mice (n = 13 donors, n = 13 recipients) by creating end-to-side anastomoses between the donors' cranial vena cava (CrVC) and the recipients' abdominal caudal vena cava (CVC), between the donors' ascending aorta and the recipients' abdominal aorta (aAo), and between the grafts' pulmonary trunk and the left atrium. After transplantation, a hemodynamic assessment using echocardiography (including 2D speckle tracking analysis) and electrocardiography was performed. Results: The loaded HHT procedure in the mice was performed with an overall success rate of 61%. In 3 of the remaining 5 cases, only atrial function was restored. The median duration of the entire surgical procedure for the recipient animal was 190 (IQR 180-250) min. The mean heart rate in the loaded HHT group was 355 ± 6 bpm in comparison to the control group with an in situ heart rate of 418 ± 61 bpm. A native-like closing and opening pattern of the aortic and mitral valves (visible on both 2D and M-mode images) was observed, confirming a native-analogous loading of the LV. Pulsed-wave Doppler provided visualization of the flow across the region of anastomoses between the pulmonary trunk and the left atrium, reaching a mean maximum velocity of 382 ± 12 mm/s. Exemplary 2D speckle tracking analysis of the LV free wall and interventricular septum revealed some differences in vector directions in one animal when compared to the orthotopic native heart, indicating an asynchronous movement of the LV. Conclusions: These results demonstrate the technical (micro)surgical feasibility of a fully loaded HHT procedure in the murine model without using a combined heart-lung transplantation approach. The acute hemodynamic performance of the HHT grafts approximated the native orthotopic situation. This model may open up new options for the investigation of cellular and molecular questions in the murine cardiovascular in vivo system in the near future.

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Section: Discussionmentioning

confidence: 99%

“…Hence, fully vascularized HHT models were developed being primarily based on a retrograde coronary and ventricular perfusion from the abdominal aorta (aAo). This heterotopic ‘unloaded' heart transplantation approach has been used in mice for experimental purposes for almost 45 years [4], and in rats for almost 50 years [5]. …”

Section: Introductionmentioning

confidence: 99%

Hemodynamic Assessment of a Murine Heterotopic Biventricularly Loaded Cardiac Transplant in vivo Model

et al. 2016

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“…Heterotopic heart transplantation was performed as de scribed [1,13] with some minor changes [3]. Briefly, anesthesia was induced with ether and maintained with halothane.…”

Section: Methodsmentioning

confidence: 99%

Active Enhancement of Heart Transplants in the Inbred Rat with Donor Strain Blood

Paola¹,

Colizza²

1975

Eur Surg Res

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Prolongation of survival time of heart transplants in inbred rats following intravenous injection of donor strain blood according to various time-dose schedules is described. The immunological status of long-term survivors shows detectable antidonor antibodies, normal graft-versus-host reactions and rejection of skin grafts in the usual time. Lymphocytotoxic activity is well related to the survival time of the heart in those rats which respond to the treatment.

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“…The most frequently applied transplant model is still the heterotopic mouse heart transplantation. This procedure was described for the first time by Corry in 1973[Corry et al, 1973 and the method was comparable to the cardiac transplantation in the rat as performed by Abbott et al in 1964[Abbott et al, 1964 and Ono et al in 1969[Ono & Lindsey, 1969.…”

Section: Potential Of Heterotopic Cardiac Transplantation In Mice As mentioning

confidence: 99%

Potential of Heterotopic Cardiac Transplantation in Mice as a Model for Elucidating Mechanisms of Graft Rejection

Laschinger¹,

Aßfalg²,

Matevossian³

et al. 2012

Cardiac Transplantation

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A Technique for Heart Transplantation In the Rat

Cited by 114 publications

References 5 publications

Hemodynamic Assessment of a Murine Heterotopic Biventricularly Loaded Cardiac Transplant in vivo Model

Hemodynamic Assessment of a Murine Heterotopic Biventricularly Loaded Cardiac Transplant in vivo Model

Active Enhancement of Heart Transplants in the Inbred Rat with Donor Strain Blood

Potential of Heterotopic Cardiac Transplantation in Mice as a Model for Elucidating Mechanisms of Graft Rejection

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