A Technique for Increasing the Number of Mitoses in the Bone Marrow of the Chinese Hamster by Injection of Pertussis Vaccine and Phytohemagglutinin

Vries, G.F. de

doi:10.1159/000230452

Cited by 6 publications

(2 citation statements)

References 10 publications

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“…The amount of lymphocytes per vene puncture can be raised by injecting the ani mal with Bordetella pertussis vaccine (HXyry and Defendi, 1970;de Vries, 1971). The proliferative potential of the lymphocytes can be enhanced in vitro by addition of 2 ME to the cultures (de Jong et al, 1977).…”

Section: Discussionmentioning

confidence: 99%

Micromethod for chromosome preparations from Chinese hamster lymphocytes

Jong¹,

Anders²,

Wierda³

et al. 1979

Cytogenet Genome Res

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Chromosome preparations from Chinese hamster lymphocytes were made by culturing for 2 to 6 days 50,000 to 800,000 lymphocytes in flat-bottomed Cooke microtiter plates in 0.1 or 0.2 ml Roswell Park Memorial Institute-1640 medium (RPMI-1640) supplemented with 5–40 µl PHA/ml, 20% fetal calf serum (FCS), and 40 µM 2-mercapto-ethanol. Depending on blood volume and cell concentrations used, it was possible to obtain up to 1400 well-spread metaphases from one venepuncture.

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Section: Discussionmentioning

confidence: 99%

Micromethod for chromosome preparations from Chinese hamster lymphocytes

Jong¹,

Anders²,

Wierda³

et al. 1979

Cytogenet Genome Res

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“…are the mean values of three (in some experiments two) replicate cultures. In the one-way cultures the stimulation index (SI) is expressed as: dprn ABx dpm AAx and in the two-way cultures as: dpm AB dpm A + dpm B t 2 Bordetella pertussis treatment of hamsters was performed, as described by De Vries (1971).…”

Section: A T E R I a L S A N D M E T H O D Smentioning

confidence: 99%

Genetics of Chinese Hamster Mixed Lymphocyte Reactivity

Jong¹,

Anders²,

Meer³

et al. 1980

Int J Immunogenetics

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Summary Two‐way and one‐way mixed lymphocyte cultures (MLC) with Chinese Hamster peripheral blood lymphocytes were performed. Reduction of the culture surface and addition of 2‐mercaptoethanol to the culture medium gives a marked enhancement of the reactivity of low numbers of lymphocytes in the MLC. Genetic analysis of the mixed lymphocyte reactivity (MLR) in the animals studied so far indicates that three or four different alleles are involved. Howerver, not all phenotypes could be traced. The samples of different stocks of Chinese Hamsters in Europe and the United States did not all share the same alleles and the samples were also different as regards the relative frequency of phenotypes. For DNA inhibition in stimulating cells mitomycin C treatment is less suitable than irradiation. Skin graft survival time was longer in animals which showed no MLR than in animals with a positive MLR.

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