A Technique for Rearing Trombiculid Mites (Acarina) Developed in a Tropical Laboratory1

Nadchatram, M.

doi:10.1093/jmedent/5.4.465

Cited by 9 publications

(7 citation statements)

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“…We have succeeded in obtaining deutonymphs, by rearing larvae on activated charcoal as substrate in order to remove plaster of Paris toxicity (Shatrov 2003). A great variety of substrate types have been tested successfully by other researchers (Wharton 1946;Wharton and Carver 1946;Michener 1946;Jenkins 1947;Farrel and Wharton 1949;Hyland 1951Hyland , 1961Lipovsky 1953Lipovsky , 1954Kaufman and Traub 1966;Nadchatram 1968;Everett et al 1973;Mallow and Crossley 1984;Takahashi et al 1993;Shatrov 1996;Tuegel and Wrenn 1998), including modifications in the proportion of activated charcoal, density of those substrates and sizes or kind of the culture containers.…”

Section: Discussionmentioning

confidence: 99%

A contribution to the knowledge of Quadraseta brasiliensis Goff and Gettinger, 1989 (Trombidiformes: Trombiculidae), with description of the deutonymph instar

et al. 2018

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In the Neotropical region the genus Quadraseta Brennan, 1970, includes 14 species, with ectoparasitic habits during the larval stage. Quadraseta brasiliensis Goff and Gettinger, 1989, was described from larvae collected on the rodent Hylaeamys megacephalus (Fisher), cited as Oryzomys capito (Olfers). According to these authors, the holotype was deposited in the Museu de Zoologia da Universidade de São Paulo and the paratypes were deposited in three other collections: Bernice Pauahi Bishop Museum, Sam Noble Oklahoma Museum of Natural History and United States National Museum of Natural History, however, no type specimens were found in any of these museums. Here we redescribe the larva, describe the deutonymph instar obtained from field-collected larvae, and report new hosts and localities for this species in Brazil. In addition we provide sequences of the 18S ribosomal RNA gene for this species.

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Section: Discussionmentioning

confidence: 99%

A contribution to the knowledge of Quadraseta brasiliensis Goff and Gettinger, 1989 (Trombidiformes: Trombiculidae), with description of the deutonymph instar

et al. 2018

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“…The mites were maintained in plastic containers (4.0 cm tall) with a plaster of paris and charcoal (9:1 ratio) substrate (1.5 cm deep). Nymphs and adults were fed Collembola (Sinella curviseta Brook) eggs, whereas larvae (chiggers) were fed on 4-wk old mice using the method of Nadchatram (1968). All mite stages were held in a biosafety cabinet at 27ЊC Ϯ 3ЊC, 50%Ð 60% RH, and natural light.…”

Section: Methodsmentioning

confidence: 99%

Development of an In Vitro Method for the Evaluation of Candidate Repellents AgainstLeptotrombidium(Acari: Trombiculidae) Chiggers

et al. 2003

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We developed a rapid and economical in vitro procedure with which to evaluate the efficacy of candidate repellents against chiggers. The procedure requires only 5 min and a small number of chiggers to obtain a valid estimate of the median effective dose. We used this procedure to evaluate the repellent activity of 11 compounds against the chigger, Leptotrombidium imphalum Vercammen-Grandjean and Langston. Median effective doses were determined for 10 of the 11 compounds. DM-165-2 (N,N-diethyl-3-flurobenzamide) was the only compound that was significantly more effective than deet.

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“…A chigger colony can be started from field-collected engorged larvae taken from trapped hosts. The larvae are allowed to detach from the host naturally to avoid killing them [ 30 ]. This will require a different IUCUC protocol for holding the wild-caught host longer than typically needed for collecting chiggers from a trapped host which are released the same day.…”

Section: Chigger Biologymentioning

confidence: 99%

“…Eggs of collembola, e.g., Sinella curviseta [ 33 ] or of mosquitoes, e.g., Culex pipiens [ 18 ], are provided ad libitum to nymphs and adults as food. Eggs before hatching are separated from the other stages by flooding the container; the eggs remain at the bottom [ 30 ]. The unfed chigger larvae are placed on a laboratory mouse in a tight-fitting wire-mesh cage where the activity of the mouse is restrained.…”

Section: Chigger Biologymentioning

confidence: 99%

Biology, Systematics, Microbiome, Pathogen Transmission and Control of Chiggers (Acari: Trombiculidae, Leeuwenhoekiidae) with Emphasis on the United States

Chen

Roe

Ponnusamy

2022

IJERPH

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Chiggers are the larval stage of Trombiculidae and Leeuwenhoekiidae mites of medical and veterinary importance. Some species in the genus Leptotrombidium and Herpetacarus vector Orientia species, the bacteria that causes scrub typhus disease in humans. Scrub typhus is a life-threatening, febrile disease. Chigger bites can also cause dermatitis. There were 248 chigger species reported from the US from almost every state. However, there are large gaps in our knowledge of the life history of other stages of development. North American wide morphological keys are needed for better species identification, and molecular sequence data for identification are minimal and not clearly matched with morphological data. The role of chiggers in disease transmission in the US is especially understudied, and the role of endosymbionts in Orientia infection are suggested in the scientific literature but not confirmed. The most common chiggers in the eastern United States were identified as Eutrombicula alfreddugesi but were likely misidentified and should be replaced with Eutrombicula cinnabaris. Scrub typhus was originally believed to be limited to the Tsutsugamushi Triangle and the chigger genus, Leptotrombidium, but there is increasing evidence this is not the case. The potential of Orientia species establishing in the US is high. In addition, several other recognized pathogens to infect humans, namely Hantavirus, Bartonella, Borrelia, and Rickettsia, were also detected in chiggers. The role that chiggers play in these disease transmissions in the US needs further investigation. It is possible some of the tick-borne diseases and red meat allergies are caused by chiggers.

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A Technique for Rearing Trombiculid Mites (Acarina) Developed in a Tropical Laboratory1

Cited by 9 publications

References 0 publications

A contribution to the knowledge of Quadraseta brasiliensis Goff and Gettinger, 1989 (Trombidiformes: Trombiculidae), with description of the deutonymph instar

A contribution to the knowledge of Quadraseta brasiliensis Goff and Gettinger, 1989 (Trombidiformes: Trombiculidae), with description of the deutonymph instar

Development of an In Vitro Method for the Evaluation of Candidate Repellents AgainstLeptotrombidium(Acari: Trombiculidae) Chiggers

Biology, Systematics, Microbiome, Pathogen Transmission and Control of Chiggers (Acari: Trombiculidae, Leeuwenhoekiidae) with Emphasis on the United States

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