A Technique of Recipient Portal Venoplasty and Cuff Insertion for Portal Revascularization in Orthotopic Rat Liver Transplantation

Macomber, Christopher W.; Shah, Shimul A.

doi:10.1016/j.jss.2011.10.047

Cited by 3 publications

(1 citation statement)

References 10 publications

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“…A heterotopic liver transplantation model was established using DA rats as donors and Lewis rats as recipients, as described previously (14,15,19). A heterotopic liver transplantation model was established using DA rats as donors and Lewis rats as recipients, as described previously (14,15,19).…”

Section: Animalsmentioning

confidence: 99%

Cotransfection with IL-10 and TGF-β1 into immature dendritic cells enhances immune tolerance in a rat liver transplantation model

Lin

Qiu

et al. 2014

American Journal of Physiology-Gastrointestinal and Liver Physi

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Dendritic cells transfected with interleukin (IL)-10 and transforming growth factor-β1 (TGF-β1) enhance T cell immunity and tolerance. However, no quantitative studies have investigated the suppressive functions of immature dendritic cells (imDC) cotransfected with IL-10 and TGF-β1. The effects of imDC cotransfected with IL-10 and TGF-β1 (IL-10-TGF-β1-imDC) on immune tolerance induction in a rat transplantation model were investigated. In addition, effects of IL-10-TGF-β1-imDC relative to IL-10-transfected imDC (IL-10-imDC) and TGF-β1-transfected imDC (TGF-β1-imDC) were compared. The infusion of IL-10-TGF-β1-imDC into recipients prolonged liver graft survival, which was sustained for >90 days. IL-12 serum levels decreased, whereas alanine transaminase and total bilirubin slightly increased in rats infused with IL-10-TGF-β1-imDC compared with the IL-10-imDC and TGF-β1-imDC groups. Furthermore, a higher percentage of terminal transferase-mediated UTP nick end-labeling-positive cells was observed, and histological analysis of the allografts indicated a rejection activity index of mild acute rejection. Our results suggest infusion of IL-10 and TGF-β1 cotransfected imDC induces alloantigen-specific T cell hyporesponsiveness, inhibits antigen-specific immunological responses to liver allografts, prolongs liver allograft survival, and enhances the immune tolerance. This approach may provide a promising alternative for enhancing donor-specific tolerance during liver transplantation.

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Section: Animalsmentioning

confidence: 99%

Cotransfection with IL-10 and TGF-β1 into immature dendritic cells enhances immune tolerance in a rat liver transplantation model

Lin

Qiu

et al. 2014

American Journal of Physiology-Gastrointestinal and Liver Physi

View full text Add to dashboard Cite

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Prolonged survival effects induced by immature dendritic cells and regulatory T cells in a rat liver transplantation model

Lin

Luo

et al. 2016

Molecular Immunology

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Increased expression of Notch 1 in a rat liver transplantation model

Huang

Lin

et al. 2015

Molecular Medicine Reports

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Liver transplantation is the standard treatment for end‑stage liver failure; however, rejection can result in allograft failure. In order to investigate the role of Notch 1 during rejection, the present study evaluated Notch 1 expression, as well as the levels of immune reactivity, in rat liver allografts. A heterotopic liver transplantation model was established using Dark Agouti (DA) rats as donors and Lewis rats as recipients (DA/Lewis), with DA recipient rats serving as controls (DA/DA). The concentration levels of immune reactivity markers and serum Notch 1 were measured on days 3, 5, and 7. The overall survival was significantly shorter (<10 days) in the DA/Lewis group, as compared with the DA/DA group (P<0.0001). The concentration levels of serum alanine aminotransferase and total bilirubin were significantly higher 5 and 7 days following transplantation in the DA/Lewis group, as compared with the DA/DA group (P<0.001). The concentration levels of serum Notch 1 were significantly higher in the DA/Lewis group, as compared with the DA/DA group on days 3, 5, and 7 following transplantation (P<0.0001). These results indicate that the expression levels of serum Notch 1 significantly increase during liver allograft rejection, suggesting that Notch 1 is involved in the mechanism underlying liver allograft rejection. Notch 1 may serve as a marker of acute rejection in a rat liver transplantation model.

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A Technique of Recipient Portal Venoplasty and Cuff Insertion for Portal Revascularization in Orthotopic Rat Liver Transplantation

Cited by 3 publications

References 10 publications

Cotransfection with IL-10 and TGF-β1 into immature dendritic cells enhances immune tolerance in a rat liver transplantation model

Cotransfection with IL-10 and TGF-β1 into immature dendritic cells enhances immune tolerance in a rat liver transplantation model

Prolonged survival effects induced by immature dendritic cells and regulatory T cells in a rat liver transplantation model

Increased expression of Notch 1 in a rat liver transplantation model

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