2019
DOI: 10.1101/556977
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A telomerase with novel non-canonical roles: TERT controls cellular aggregation and tissue size inDictyostelium

Abstract: 11Telomerase, particularly its main subunit, the reverse transcriptase, TERT, prevents DNA 12 erosion during eukaryotic chromosomal replication, but also has poorly understood non-13 canonical functions. Here, in the model social amoeba Dictyostelium discoideum, we show 14 that the protein encoded by tert has telomerase-like motifs and regulates, non-canonically, 15 important developmental processes. Expression levels of wild-type (WT) tert were biphasic, 16 peaking at 8 and 12 h post-starvation, aligning with… Show more

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Cited by 3 publications
(2 citation statements)
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“…The culturing of Dictyostelium and the developmental assays including chemotaxis and cell adhesion assays were carried out as described by Nassir et al 39 or Lam et al 40 . Cells used in this study were from Ax2 background that were grown in modi ed maltose-HL5 medium (Formedium, UK).…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…The culturing of Dictyostelium and the developmental assays including chemotaxis and cell adhesion assays were carried out as described by Nassir et al 39 or Lam et al 40 . Cells used in this study were from Ax2 background that were grown in modi ed maltose-HL5 medium (Formedium, UK).…”
Section: Methodsmentioning
confidence: 99%
“…Serotonin was tested from 100 nM to 1 mM on wild-type cells and 500 µM concentration was found to be the effective dose in causing the mound arrest phenotype. The concentrations of SQ22536 (2 mM), H89 (50 µM), 8-Br-cAMP (5 mM), cAMP (0.2 mM), c-di-GMP (1 mM), DIF (200 nM), adenosine (2 mM), adenosine deaminase (5U), caffeine (2 mM) and IBMX (0.5mM) treatment were based on the literature 24,39,47,48 . All the ne chemicals were from Sigma-Aldrich, USA except as indicated.…”
mentioning
confidence: 99%