A temporary immersion system improves in vitro regeneration of peach palm through secondary somatic embryogenesis

Abstract

The present study confirms the occurrence of secondary somatic embryos in peach palm and describes a feasible protocol for regeneration of peach palm in vitro. Further optimizations include the use of explants obtained from adult palms and improvement of somatic embryo conversion rates.

Histological analysis and transcription profiles on somatic embryogenesis in interspecific hybrids of <i>Elaeis guineensis × E. oleifera</i>

“…"Ear"-like structures were reported decades ago for E. guineensis [8] and observed in coconut [15] and Bactris [23]. In the present study, they became visible during the initial months of cultivation and again following the reduction in auxin concentration, which induced culture 511 to revert throughout the route for embryogenesis to produce callus line 511A.…”

“… Callus lines 509 and 002 were highly embryogenic cultures collected from multiplication/maturation medium supplemented with 8 mg·l −1 2,4-D 18 months after the introduction of zygotic embryos in vitro.  Callus line 511B was obtained by transferring "ear"-like embryogenic structures excised from 511A for 2.4 mg·l −1 Picloram in basal medium free of charcoal [23] for two months followed by additional three months on basal medium free of growth regulators and charcoal. Immediately after registration of the morphological aspects through high definition digital images, biological material (calli and/or more friable cultures) was organized as individual 100 mg samples, immediately frozen in liquid nitrogen and preserved at −80˚C.…”

Peach palm (Bactris gasipaes) in tropical Latin America: implications for biodiversity conservation, natural resource management and human nutrition

“…Only in one collection have clones been selected for propagation (Table 2). Nevertheless, research is underway to further improve techniques, such as somatic embryogenesis, for clonal propagation (Steinmacher et al 2007(Steinmacher et al , 2011. In contrast to cultivated peach palm, wild populations (being important resources for genetic improvement) are threatened by deforestation, driven mainly by agricultural expansion and the transition of forest to savannah ).…”

Cellular Markers for Somatic Embryogenesis

“…The most important question concerns the mechanisms underlying the changes (the transition) of the differentiated state of the plant cell into a totipotent and finally an embryogenic state . It was documented that during DSE somatic cells acquire their embryogenic competence through dedifferentiation (Harada, 1999;Fehér et al, 2003;Steinmacher et al, 2011). Such big changes in cell fate depend on the possibility of acquiring the ability to divide (Nagata, 2010).…”