A Terrified Sound Stress Impairs Learning And Memory Ability of Adult Female

Abstract

Stress, as an important environmental factor of mental health, cannot be ignored. The great physiological difference between males and females implies that the effects of stress may differ by gender. However, few studies have focused on the effects of stress on females. This study investigated the effects of a terrified sound stress on adult female mice.Methods: 32 adults female C57BL/6 mice were randomly divided into control group (n=16) and stress group (n=16). Sucrose preference test and open field test (OFT) were carried out to evaluate the anxiety and depression of mice. Spatial learning and memory ability were measured by Morris Water maze test (MWM). Endocrine hormones were determined by enzyme-linked immunosorbent assay (ELISA). Serum differential proteins were screened by mass spectrometry (MS). Results: Compared with control group, the sucrose preference of stress group was decreased; in MWM, the escape latency of the stress group was significantly prolonged (P<0.05), and the total swimming distance was significantly increased (P<0.05).Serum T (P<0.05), GnRH (P<0.05), FSH and LH levels decreased; thirty six differential peaks were found by MS, eight of them had high multiples of difference (> 1.2 or <0.8). Conclusion: terrified sound stress impairs spatial learning ability and mental health of adult female mice.