A test of four candidate barcoding markers for the identification of geographically widespread<i>Chimaphila</i>species (Pyroleae, Ericaceae)

Liu, Zhen Wen; Zhao, Qian

doi:10.1080/12538078.2013.773462

Cited by 2 publications

(3 citation statements)

References 27 publications

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“…In conclusion, we support the use of the ITS marker as a supplementary barcode in plants, while the performance of ITS should be evaluated in extensive trials in different plant groups (Liu et al, 2013; Liu J. et al, 2014; Wirta et al, 2016). The pragmatic solution to a complex trade-off between universality, sequence quality, discrimination, and cost, is the combination of T+I (90.5% species resolution), the most efficient and economical marker among single or combined DNA barcodes when identifying tree species in Aceraceae.…”

Section: Discussionsupporting

confidence: 52%

“…With the development of molecular biology, the rise of DNA barcoding is expected to mitigate this dilemma, at least in part. DNA barcoding is one method that uses standard molecular techniques to achieve rapid and accurate species identification by a short DNA sequence (Hebert et al, 2003; Hebert and Gregory, 2005; Liu et al, 2013; Krawczyk et al, 2014). This method to remedy the limitations of a traditional morphology-based identification system will allow for more rapid progress in traditional taxonomic work (Kress et al, 2005; Liu J. et al, 2014; Nigro et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

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Efficient Identification of the Forest Tree Species in Aceraceae Using DNA Barcodes

Han

Duan

et al. 2016

Front. Plant Sci.

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Aceraceae is a large forest tree family that comprises many economically and ecologically important species. However, because interspecific and/or intraspecific morphological variations result from frequent interspecific hybridization and introgression, it is challenging for non-taxonomists to accurately recognize and identify the tree species in Aceraceae based on a traditional approach. DNA barcoding is a powerful tool that has been proposed to accurately distinguish between species. In this study, we assessed the effectiveness of three core standard markers (matK, rbcL and ITS) plus the chloroplast locus trnS-trnG as Aceraceae barcodes. A total of 231 sequences representing 85 species in this forest family were collected. Of these four barcode markers, the discrimination power was highest for the ITS (I) region (50%) and was progressively reduced in the other three chloroplast barcodes matK (M), trnS-trnG (T) and rbcL (R); the discrimination efficiency of the ITS marker was also greater than any two-locus combination of chloroplast barcodes. However, the combinations of ITS plus single or combined chloroplast barcodes could improve species resolution significantly; T+I (90.5% resolution) and R+M+T+I (90.5% resolution) differentiated the highest portion of species in Aceraceae. Our current results show that the nuclear ITS fragment represents a more promising DNA barcode marker than the maternally inherited chloroplast barcodes. The most efficient and economical method to identify tree species in Aceraceae among single or combined DNA barcodes is the combination of T+I (90.5% resolution).

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Section: Discussionsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 99%

Efficient Identification of the Forest Tree Species in Aceraceae Using DNA Barcodes

Han

Duan

et al. 2016

Front. Plant Sci.

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“…Panax ginseng C.A.Mey. [ 29 30 ], Dendrobium Species[ 25 26 31 ], Euphorbia pekinensis [ 32 ], Bupleurum species[ 33 ], Chimaphila species[ 34 ] and Gentianopsis paludosa (Hook. f.) Ma[ 24 ] are all successful examples.…”

Section: Discussionmentioning

confidence: 99%

Molecular authentication of the traditional medicinal plantPeucedanum praeruptorumand its substitutes and adulterants by dna 0 - barcoding technique

Wang

Liu

2014

Phcog Mag

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Background:Peucedanum praeruptorum L., a traditional Chinese medicine known as Qian-hu, is commonly used for dispelling wind-heat and expectorant and loss of energy. However, due to similar morphological characters and high market demand, there are many substitutes and adulterants of P. praeruptorum. DNA barcoding is an approach to identify species based on sequences from a short, standardized DNA region.Objective:To authenticate P. praeruptorum from its substitutes and adulterants.Materials and Methods:The differential identification of P. praeruptorum and 13 regional substitutes and 23 adulterants was investigated by means of DNA sequence analysis of internal transcribed spacer (ITS) region of the nuclear ribosomal DNA (nrDNA), a bootstrap neighbor-joining (NJ) tree according to Kimura's 2-parameter method was also calculated.Results:The data showed that P. praeruptorum, its substitutes and adulterants could be easily distinguished at the DNA level, while almost all species were well resolved, and successfully identified on the NJ tree.Conclusion:The ITS sequence can be used for the identification of P. praeruptorum and to distinguish it from common substitutes and adulterants.

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A test of four candidate barcoding markers for the identification of geographically widespreadChimaphilaspecies (Pyroleae, Ericaceae)

Cited by 2 publications

References 27 publications

Efficient Identification of the Forest Tree Species in Aceraceae Using DNA Barcodes

Efficient Identification of the Forest Tree Species in Aceraceae Using DNA Barcodes

Molecular authentication of the traditional medicinal plantPeucedanum praeruptorumand its substitutes and adulterants by dna 0 - barcoding technique

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