AbstractDesign is inherently affected by human-related factors and it is of no surprise that the fine-tuning of instruments capable of measuring aspects of human behavior has attracted interest in the design field. The recalled instruments include a variety of devices that capture and quantitatively assess people's unintentional and unconscious reactions and that are generally referred as neurophysiological or biometric. The number of experimental applications of these instruments in design was extremely limited as of 2016, when Lohmeyer and Meboldt published a first report on relevant measures and their interpretation in design. In the last few years, the number of relevant publications has increased dramatically and this determines the opportunity to carry out a comprehensive review in the field. The reviewed contributions are analyzed and classified according to, among others, instruments used, the kind of stakeholders involved and the supported design research activities. The role of biometric measures with respect to traditional research methods is emphasized too. The discussed instruments can represent supports or substitutes for traditional approaches, as well as they are capable of exploring phenomena that could not be addressed hitherto. The intensity of research concerning experiments with biometric measurements is discussed too; a particular focus of the final discussion is the individuation of obstacles that prevent them from becoming commonplace in design research.