A test paper for the detection of galactose and certain galactose-containing sugars

Rorem, Edward S.; Lewis, John B.

doi:10.1016/0003-2697(62)90059-3

Cited by 15 publications

(4 citation statements)

References 5 publications

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“…( 2) Its rate of hydrolysis by acid is seven times greater than that of a ß-D-galactopyranoside, and is clearly in the range expected of a galactofuranoside. (3) It fails to react with galactose oxidase, a property shared by ethyl ß-D-galactofuranoside, but not by D-galactopyranosides (Rorem and Lewis, 1962). (4) Its reactions with periodate are compatible only with a furanoside structure.…”

Section: Discussionmentioning

confidence: 99%

A Glycerol Galactofuranoside from the Lipid of an Anaerobe^*

Reeves¹,

Latour²,

Lousteau³

1964

Biochemistry

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The lipid from an anaerobic organism provisionally designated Bacteroides symbiosus was found to contain D-galactose. Alkaline hydrolysis of the lipid yielded a new glycerol galactoside, specific rotation -73°, with a crystalline hexabenzoate, mp 133-134°, specific rotation -6°. The properties of the new galactoside indicate it to be a 1-glycerol ß-D-galactofuranoside. This appears to be the first recorded recognition of a naturally occurring galactofuranoside.An anaerobic organism provisionally designated Bacteroides symbiosus is consumed in quantity by Entamoeba histolytica during amebal growth in the Shaffer-Frye culture systems (Shaffer, 1952;Reeves et al., 1957). Our interest in determining the lipid requirements for amebal growth led to a study of the composition of the bacterial lipid. This report deals with the finding of a new glycerol galactoside in the lipid.

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Section: Discussionmentioning

confidence: 99%

A Glycerol Galactofuranoside from the Lipid of an Anaerobe^*

Reeves¹,

Latour²,

Lousteau³

1964

Biochemistry

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“…Since the last century, paper, generally made of cellulose fibers, has been used as a platform for chromatographic separations, 1 chemical, 2 and biochemical tests. 3,4 The main attractive features of paper are its low-cost, high abundance, elevated porosity, biodegradability, and excellent chemical compatibility with many applications. Recently, microfluidic paper-based analytical devices (mPADs) were firstly reported by Whitesides' group 5,6 as cheap platforms for lab-on-paper applications.…”

Section: Introductionmentioning

confidence: 99%

3D Origami-based multifunction-integrated immunodevice: low-cost and multiplexed sandwich chemiluminescence immunoassay on microfluidic paper-based analytical device

et al. 2012

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A novel 3D microfluidic paper-based immunodevice, integrated with blood plasma separation from whole blood samples, automation of rinse steps, and multiplexed CL detections, was developed for the first time based on the principle of origami (denoted as origami-based device). This 3D origami-based device, comprised of one test pad surrounded by four folding tabs, could be patterned and fabricated by wax-printing on paper in bulk. In this work, a sandwich-type chemiluminescence (CL) immunoassay was introduced into this 3D origami-based immunodevice, which could separate the operational procedures into several steps including (i) folding pads above/below and (ii) addition of reagent/buffer under a specific sequence. The CL behavior, blood plasma separation, washing protocol, and incubation time were investigated in this work. The developed 3D origami-based CL immunodevice, combined with a typical luminuol-H(2)O(2) CL system and catalyzed by Ag nanoparticles, showed excellent analytical performance for the simultaneous detection of four tumor markers. The whole blood samples were assayed and the results obtained were in agreement with the reference values from the parallel single-analyte test. This paper-based microfluidic origami CL detection system provides a new strategy for a low-cost, sensitive, simultaneous multiplex immunoassay and point-of-care diagnostics.

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“…Galactose oxidase has been found to be a helpful analytical tool for the specific determination of D-galactose in blood plasma (DeVerdier and Hjelm, 1962), in plant extracts (Rorem and Lewis, 1962), and in phospholipids (Agranoff, Radin, and Suomi, 1962;Bradley and Kanfer, 1964), and was highly useful for the characterization of terminal D-galactoside units in several polymers (Avigad et al, 1962;Blumenfeld et al, 1963;Barker, Pardoe, and Stacey, 1963;Osborn et al, 1964;Robinson and Pierce, 1964).…”

mentioning

confidence: 99%

Effect of Culture Conditions on the Production of d-Galactose Oxidase by Dactylium dendroides1

Markus¹,

Miller²,

Avigad³

1965

Applied Microbiology

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Effect of culture conditions on the production of D-galactose oxidase by Dactylium dendroides. Appl. Microbiol. 13:686-693. 1965.-The effects on enzyme production of inoculum size and age, medium composition, and culture conditions were studied in shake flasks and in a pilot-plant fermentor. Using a medium consisting of glucose, yeast extract, and inorganic salts in deionized water, we found that the addition of Cu++ was essential for the formation of active enzyme. Cultures grown in the absence of added copper produced an inactive enzyme protein which could be activated by 103 M Cu+. Thiamine fulfilled all requirements for exogenous vitamins for growth and enzyme production. Glucose concentrations higher than 1% markedly suppressed enzyme formation. The mycelium inactivated the enzyme on prolonged incubation of the culture. Mycelial autolysates and sonic extracts were found to contain a thermostable and slowly dialyzable galactose oxidase-inactivating factor. The experiments suggest that this factor operates as a chelating agent which forms complexes with the copper of the enzyme. Copper ions (10I8 M) prevented enzyme inactivation and restored activity to samples previously inactivated by this factor.

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A test paper for the detection of galactose and certain galactose-containing sugars

Cited by 15 publications

References 5 publications

A Glycerol Galactofuranoside from the Lipid of an Anaerobe^*

A Glycerol Galactofuranoside from the Lipid of an Anaerobe^*

3D Origami-based multifunction-integrated immunodevice: low-cost and multiplexed sandwich chemiluminescence immunoassay on microfluidic paper-based analytical device

Effect of Culture Conditions on the Production of d-Galactose Oxidase by Dactylium dendroides1

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A test paper for the detection of galactose and certain galactose-containing sugars

Cited by 15 publications

References 5 publications

A Glycerol Galactofuranoside from the Lipid of an Anaerobe*

A Glycerol Galactofuranoside from the Lipid of an Anaerobe*

3D Origami-based multifunction-integrated immunodevice: low-cost and multiplexed sandwich chemiluminescence immunoassay on microfluidic paper-based analytical device

Effect of Culture Conditions on the Production of d-Galactose Oxidase by Dactylium dendroides1

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A Glycerol Galactofuranoside from the Lipid of an Anaerobe^*

A Glycerol Galactofuranoside from the Lipid of an Anaerobe^*