A tetraspanin regulates septate junction formation in <i>Drosophila</i> midgut

Izumi, Yasushi; Motoishi, Minako; Furuse, Kyoko; Furuse, Mikio

doi:10.1242/jcs.180448

Cited by 56 publications

(101 citation statements)

References 38 publications

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“…Among the 13 genes contained in this chromosomal region, we focused on one gene encoding a membrane protein, designated Tsp2A, because the Flybase indicated that it is highly expressed in the midgut and Malpighian tubules. Through immunological studies using the antibodies generated against a synthetic polypeptide corresponding to the C‐terminal cytoplasmic domain of Tsp2A, we found that Tsp2A exclusively localizes to sSJs in the midgut and Malpighian tubules …”

Section: Identification Of Ssj‐associated Proteins Using a Genetic Apmentioning

confidence: 99%

“…Immunolocalization studies revealed that the three membrane proteins Ssk, Mesh, and Tsp2A were exclusively located at sSJs in the midgut and Malpighian tubules of Drosophila (Fig. ) . Ssk consists of 162 amino acids and has four transmembrane domains.…”

Section: Molecular Structures and Functions Of Ssk Mesh And Tsp2amentioning

confidence: 99%

“…Tsp2A consists of 244 amino acids and belongs to the tetraspanin family of proteins, which are membrane proteins widely conserved in metazoans with four transmembrane domains. While tetraspanins have been reported to show a wide variety of functions, Tsp2A is the first observed to accumulate highly at cell–cell junctions . Figure depicts these sSJ‐specific membrane proteins and other sSJ‐associated proteins.…”

Section: Molecular Structures and Functions Of Ssk Mesh And Tsp2amentioning

confidence: 99%

“…A schematic drawing of sSJ‐associated proteins in the Drosophila midgut. In addition to Ssk, Mesh, and Tsp2A, the adhesion molecule Fas III and three plaque proteins––Dlg, Coracle, and Lgl––are known to localize to sSJs …”

Section: Molecular Structures and Functions Of Ssk Mesh And Tsp2amentioning

confidence: 99%

“…Ssk, Mesh, and Tsp2A cooperatively contribute to sSJ formation . Immunoprecipitation analyses showed that all three proteins coprecipitated as a protein complex, although the manners of their interactions remained unclear.…”

Section: Molecular Structures and Functions Of Ssk Mesh And Tsp2amentioning

confidence: 99%

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Molecular dissection of smooth septate junctions: understanding their roles in arthropod physiology

Furuse

Izumi

2017

Annals of the New York Academy of Sciences

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Smooth septate junctions (sSJs) are cell-cell junctions that are thought to regulate the paracellular pathway of the intestine and renal system in arthropods. The detailed mechanism of action of sSJs is not well understood, because their molecular organization has remained elusive for a long time. Recently, two sSJ-specific membrane proteins, Ssk and Mesh, were identified by screening monoclonal antibodies raised against sSJ-containing membrane fractions isolated from the silkworm midgut. Furthermore, a genetic screen in Drosophila based on microscopic observation of sSJ formation identified Tsp2A as a novel sSJ-specific membrane protein. Together with Tsp2A, Ssk and Mesh form a protein complex, and all three proteins are required for sSJ formation, as well as intestinal barrier function in Drosophila. Additional studies are likely to elucidate their roles in (1) the formation and reorganization of sSJs, (2) paracellular barrier functions and permselectivity, and (3) short-term and long-term regulation of paracellular functions in arthropod epithelia.

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Section: Identification Of Ssj‐associated Proteins Using a Genetic Apmentioning

confidence: 99%

Section: Molecular Structures and Functions Of Ssk Mesh And Tsp2amentioning

confidence: 99%

Section: Molecular Structures and Functions Of Ssk Mesh And Tsp2amentioning

confidence: 99%

Section: Molecular Structures and Functions Of Ssk Mesh And Tsp2amentioning

confidence: 99%

Section: Molecular Structures and Functions Of Ssk Mesh And Tsp2amentioning

confidence: 99%

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Molecular dissection of smooth septate junctions: understanding their roles in arthropod physiology

Furuse

Izumi

2017

Annals of the New York Academy of Sciences

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Requirement of Snakeskin for normal functions of midgut and Malpighian tubules in Henosepilachna vigintioctopunctata

Zhang

Yang

Jin

et al. 2023

Arch Insect Biochem Physiol

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Septate junctions (SJs) are located between epithelial cells and play crucial roles in epithelial barrier formation and epithelia cell homeostasis. Nevertheless, the molecular constituents, especially those related to smooth SJs (sSJs), have not been well explored in non‐Drosophilid insects. A putative integral membrane protein Snakeskin (Ssk) was identified in a Coleoptera foliar pest Henosepilachna vigintioctopunctata. RNA interference‐aided knockdown of Hvssk at the third‐instar larval stage arrested larval development. Most resultant larvae failed to shed larval exuviae until their death. Silence of Hvssk at the fourth‐instar larvae inhibited the growth and reduced foliage consumption. Dissection and microscopic observation revealed that compromised expression of Hvssk caused obvious phenotypic defects in the midgut. A great number of morphologically abnormal columnar epithelial cells accumulated throughout the midgut lumen. Moreover, numerous vesicles were observed in the malformed cells of the Malpighian tubules (Mt). All the Hvssk depleted larvae remained as prepupae; they gradually darkened and eventually died. Furthermore, depletion of Hvssk at the pupal stage suppressed adult feeding and shortened adult lifespan. These findings demonstrated that Ssk plays a vital role in the integrity and function of both midguts and Mt, and established the conservative roles of Ssk in the formation of epithelial barrier and the homeostasis of epithelial cells in H. vigintioctopunctata.

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Txp40, a protein from Photorhabdus akhurstii, conferred potent insecticidal activity against the larvae of Helicoverpa armigera, Spodoptera litura and S. exigua

Shankhu

Mathur

Mandal

et al. 2020

Pest Management Science

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BACKGROUND: Txp40, a 37 kDa protein, previously characterized from the Gram-negative bacterium Photorhabdus akhurstii (symbiotically associates with insect-parasitic nematode, Heterorhabditis indica), conferred insecticidal activity against Galleria mellonella. Here, the biological activity of Txp40 was evaluated against economically important insects, including Helicoverpa armigera, Spodoptera litura and S. exigua.RESULTS: When both intra-hemocoel injected and orally fed to test insects, comparatively greater oral LD 50 (187.7-522 ng g −1 ) than injection LD 50 (32.33-150.6 ng g −1 ) was obtained with Txp40 derived from P. akhurstii strain IARI-SGMG3

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A tetraspanin regulates septate junction formation in Drosophila midgut

Cited by 56 publications

References 38 publications

Molecular dissection of smooth septate junctions: understanding their roles in arthropod physiology

Molecular dissection of smooth septate junctions: understanding their roles in arthropod physiology

Requirement of Snakeskin for normal functions of midgut and Malpighian tubules in Henosepilachna vigintioctopunctata

Txp40, a protein from Photorhabdus akhurstii, conferred potent insecticidal activity against the larvae of Helicoverpa armigera, Spodoptera litura and S. exigua

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