A theoretical model for single blastocyst transfer

Teranishi, A.; Kuwata, A.; Fumino, T.; Hamai, H.; Shigeta, Minoru

doi:10.1007/s10815-009-9321-y

Cited by 11 publications

(9 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Therefore, single-embryo transfer was proposed. Furthermore, elective blastocyst culture and single blastocyst transfer have been demonstrated as the best way to achieve this goal [ 9 10 11 ]. However, blastocyst culture is not suitable for all patients, so choosing patients appropriately is critical.…”

Section: Discussionmentioning

confidence: 99%

Influence of the insemination method on the outcomes of elective blastocyst culture

Wang

Feng

Zhang

et al. 2017

Clin Exp Reprod Med

View full text Add to dashboard Cite

ObjectiveThe aim of this study was to explore the effects of the insemination method on the outcomes of elective blastocyst culture.MethodsWe retrospectively analyzed the outcomes of elective blastocyst culture performed between January 2011 and December 2014.ResultsThere were 2,003 cycles of conventional in vitro fertilization (IVF) and 336 cycles of intracytoplasmic sperm injection (ICSI), including 25,652 and 4,164 embryos that underwent sequential blastocyst culture, respectively. No significant differences were found in the female patients' age, basal follicle-stimulating hormone level, basal luteinizing hormone level, body mass index, number of oocytes, maturity rate, fertilization rate, or good-quality embryo rate. However, the blastocyst formation rate and embryo utilization rate were significantly higher in the conventional IVF group than in the ICSI group (54.70% vs. 50.94% and 51.09% vs. 47.65%, respectively, p<0.05). The implantation/pregnancy rate (IVF, 50.93%; ICSI, 55.10%), miscarriage rate (IVF, 12.57%; ICSI, 16.29%), and live birth rate (IVF, 42.12%; ICSI, 44.08%) were similar (p>0.05). No cycles were canceled due to the formation of no usable blastocysts.ConclusionAlthough the fertilization method had no effect on clinical outcomes, the blastocyst formation rate and embryo utilization rate in the ICSI group were significantly lower than those observed in the conventional IVF group. Therefore, more care should be taken when choosing to perform blastocyst culture in ICSI patients.

show abstract

Section: Discussionmentioning

confidence: 99%

Influence of the insemination method on the outcomes of elective blastocyst culture

Wang

Feng

Zhang

et al. 2017

Clin Exp Reprod Med

View full text Add to dashboard Cite

show abstract

“…Conventional IVF procedures were performed using previously described routine methods [13]. Embryo assessment was performed on days 3 and 5 for all study groups according to previously published criteria, including cell number, fragmentation, and morphological aspects.…”

Section: Embryo Assessmentmentioning

confidence: 99%

Mitochondrial DNA copy number in cumulus cells is a strong predictor of obtaining good-quality embryos after IVF

Ogino

Taniguchi

Sakata

et al. 2016

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose The aim of this study was to establish a simple tool to predict good-quality embryos in in vitro fertilization (IVF) by using cumulus cells (CCs) or peripheral blood cells (PBCs). Methods Mitochondrial DNA was extracted from CCs and PBCs in patients undergoing IVF. Using real-time polymerase chain reaction, mtDNA copy number in a single cell was calculated. Embryo quality was assessed when it was transferred or frozen. Results CCs were obtained from 60 oocyte cumulus-cell complexes (OCCCs) in 30 women, and PBCs were collected from 18 women. For the 30 women in the study, the median age was 37 years old (range, 24-43), and the mean body mass index was 21.4 (standard error, 2.0). mtDNA content of CCs and PBCs was highly correlated (Pearson's r = 0.900, p < 0.0001). The median mtDNA content of CCs for goodand poor-quality embryos was 140 and 57, respectively (p < 0.0001). The median mtDNA content of PBCs for goodand poor-quality embryos was 36 and 13, respectively (p = 0.604). The logistic regression model indicated that mtDNA content in CCs was the only parameter that predicted good-quality embryos (p = 0.020). The receiver operating characteristic curve for obtaining good-quality embryos by mtDNA copy number in CCs had an area under the curve of 0.823, and using a threshold of 86, positive and negative predictive values were 84.4 and 82.1 %, respectively. Conclusions The determination of mtDNA content in CCs can be used to predict good-quality embryos.

show abstract

“…Embryos with grade ≥3BB serve as suitable candidates for single embryos transfer. 21 Specifically, 3BB stands for “degree of expansion and hatching status with a full blastocyst with a blastocoel completely filling the embryo; inner cell mass with loosely grouped, several cells; Tropoderm with few cells forming a loose epithelium.” 21 On D5, 35 derived blastocysts that scored better than 3BB were cryopreserved for future transfer; the remaining 26 blastocysts were transferred to new medium and cultured to D6 prior to cryopreservation. The spent culture media from D5 to D6 were collected and underwent cell-free DNA quantification.…”

Section: Methodsmentioning

confidence: 99%

Medium-Based Noninvasive Preimplantation Genetic Diagnosis for Human α-Thalassemias-SEA

Ding

Shen

et al. 2015

Medicine

View full text Add to dashboard Cite

To develop a noninvasive medium-based preimplantation genetic diagnosis (PGD) test for α-thalassemias-SEA.The embryos of α-thalassemia-SEA carriers undergoing in vitro fertilization (IVF) were cultured. Single cells were biopsied from blastomeres and subjected to fluorescent gap polymerase chain reaction (PCR) analysis; the spent culture media that contained embryo genomic DNA and corresponding blastocysts as verification were subjected to quantitative-PCR (Q-PCR) detection of α-thalassemia-SEA. The diagnosis efficiency and allele dropout (ADO) ratio were calculated, and the cell-free DNA concentration was quantitatively assessed in the culture medium.The diagnosis efficiency of medium-based α-thalassemias–SEA detection significantly increased compared with that of biopsy-based fluorescent gap PCR analysis (88.6% vs 82.1%, P < 0.05). There is no significant difference regarding ADO ratio between them. The optimal time for medium-based α-thalassemias–SEA detection is Day 5 (D5) following IVF.Medium-based α-thalassemias–SEA detection could represent a novel, quick, and noninvasive approach for carriers to undergo IVF and PGD.

show abstract

A theoretical model for single blastocyst transfer

Cited by 11 publications

References 27 publications

Influence of the insemination method on the outcomes of elective blastocyst culture

Influence of the insemination method on the outcomes of elective blastocyst culture

Mitochondrial DNA copy number in cumulus cells is a strong predictor of obtaining good-quality embryos after IVF

Medium-Based Noninvasive Preimplantation Genetic Diagnosis for Human α-Thalassemias-SEA

Contact Info

Product

Resources

About