A theoretical model of dentinogenesis: Dentin and dentinal tubule formation

Nino-Barrera, Javier; Gutiérrez, María Lucía Lahoz; Garzón‐Alvarado, Diego Alexander

doi:10.1016/j.cmpb.2013.06.010

Cited by 10 publications

(4 citation statements)

References 26 publications

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“…The hardness of a tissue determines its resistance to deformation, scratching, or abrasion. Additionally, hardness is described as the capability to resist permanent indentation 35. In the case of teeth, it is a valid indicator to assess the tooth or dentin resistance to fracture.…”

Section: Resultsmentioning

confidence: 99%

<p>Phenotypic Properties of Collagen in Dentinogenesis Imperfecta Associated with Osteogenesis Imperfecta</p>

Ibrahim¹,

Strange²,

Aguayo³

et al. 2019

IJN

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IntroductionDentinogenesis imperfecta type 1 (OIDI) is considered a relatively rare genetic disorder (1:5000 to 1:45,000) associated with osteogenesis imperfecta. OIDI impacts the formation of collagen fibrils in dentin, leading to morphological and structural changes that affect the strength and appearance of teeth. However, there is still a lack of understanding regarding the nanoscale characterization of the disease, in terms of collagen ultrastructure and mechanical properties. Therefore, this research presents a qualitative and quantitative report into the phenotype and characterization of OIDI in dentin, by using a combination of imaging, nanomechanical approaches.MethodsFor this study, 8 primary molars from OIDI patients and 8 primary control molars were collected, embedded in acrylic resin and cut into longitudinal sections. Sections were then demineralized in 37% phosphoric acid using a protocol developed in-house. Initial experiments demonstrated the effectiveness of the demineralization protocol, as the ATR-FTIR spectral fingerprints showed an increase in the amide bands together with a decrease in phosphate content. Structural and mechanical analyses were performed directly on both the mineralized and demineralized samples using a combination of scanning electron microscopy, atomic force microscopy, and Wallace indentation.ResultsMesoscale imaging showed alterations in dentinal tubule morphology in OIDI patients, with a reduced number of tubules and a decreased tubule diameter compared to healthy controls. Nanoscale collagen ultrastructure presented a similar D-banding periodicity between OIDI and controls. Reduced collagen fibrils diameter was also recorded for the OIDI group. The hardness of the (mineralized) control dentin was found to be significantly higher (p<0.05) than that of the OIDI (mineralized) dentine. Both the exposed peri- and intratubular dentinal collagen presented bimodal elastic behaviors (Young’s moduli). The control samples presented a stiffening of the intratubular collagen when compared to the peritubular collagen. In case of the OIDI, this stiffening in the collagen between peri- and intratubular dentinal collagen was not observed and the exposed collagen presented overall a lower elasticity than the control samples.ConclusionThis study presents a systematic approach to the characterization of collagen structure and properties in OIDI as diagnosed in dentin. Structural markers for OIDI at the mesoscale and nanoscale were found and correlated with an observed lack of increased elastic moduli of the collagen fibrils in the intratubular OIDI dentin. These findings offer an explanation of how structural changes in the dentin could be responsible for the failure of some adhesive restorative materials as observed in patients affected by OIDI.

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Section: Resultsmentioning

confidence: 99%

<p>Phenotypic Properties of Collagen in Dentinogenesis Imperfecta Associated with Osteogenesis Imperfecta</p>

Ibrahim¹,

Strange²,

Aguayo³

et al. 2019

IJN

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“…As odontoblasts migrate towards the pulp, they leave behind an extension referred to as the odontoblastic process, which then penetrates the predentin. At this stage, predentin is not mineralized and is composed of a collagen network, which gradually becomes mineralized as the extension of the odontoblastic process results in protein secretion necessary for the mineralization process [18]. With mutations in the genes encoding collagen, dentin defects may occur [13].…”

Section: Discussionmentioning

confidence: 99%

Morphological Characterization of Deciduous Enamel and Dentin in Patients Affected by Osteogenesis Imperfecta

et al. 2020

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The purpose of this study was to clarify the structural and ultrastructural alterations of the enamel and dentin collagen network in the deciduous teeth of children affected by osteogenesis imperfecta (OI) using field-emission in-lens scanning electron microscopy (FEI-SEM) and transmission electron microscopy (TEM) analyses. Exfoliated primary teeth were collected from children with a diagnosis of OI and from healthy individuals (N = 24). Tooth slices containing both dentin and enamel were fixed, dehydrated and dried, gold sputtered, and observed using FEI-SEM. Additional dentin fragments were decalcified, dehydrated, embedded in resin, cut, and processed for TEM analysis. Under FEI-SEM, the enamel in OI-affected children showed an irregular prism distribution with the enamel hydroxyapatite crystals unpacked. Ultrastructural correlative analysis of the dentin in patients affected by OI showed an altered collagen pattern with a low density. In some areas, teeth in OI patients showed a reduction in the number of dentinal tubules, with odontoblastic process missing in most of the tubules. The presence of altered dentine and enamel organization in OI children was firmly established at an ultrastructural level, but additional biochemical studies are necessary in order to clarify quantitatively and qualitatively the collagenic and non-collagenic proteins in this disorder.

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“…These observations have been useful to the understanding of the carious process in dentine. However, little is known about how the mechanical properties degrade as a result of the acid dissolution [24]. In this paper, we first develop an analytical composite model of human dentine in relation to its unique PTD and ITD microstructure and then establish five 3D numerical representative volume elements (RVEs) in two groups with variations in either PTD fraction or dentinal tubule density to determine how the effective Young's modulus would degrade, to calculate mechanical response, in particular the effective stress redistribution subject to a mastication-like load, and to evaluate the critical factors that can influence the mechanical performance, as a consequence of acid dissolution.…”

Section: Introductionmentioning

confidence: 99%

Insights into the reinforcement role of peritubular dentine subjected to acid dissolution

Sui

2020

Journal of the Mechanical Behavior of Biomedical Materials

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A theoretical model of dentinogenesis: Dentin and dentinal tubule formation

Cited by 10 publications

References 26 publications

<p>Phenotypic Properties of Collagen in Dentinogenesis Imperfecta Associated with Osteogenesis Imperfecta</p>

<p>Phenotypic Properties of Collagen in Dentinogenesis Imperfecta Associated with Osteogenesis Imperfecta</p>

Morphological Characterization of Deciduous Enamel and Dentin in Patients Affected by Osteogenesis Imperfecta

Insights into the reinforcement role of peritubular dentine subjected to acid dissolution

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