A Thermal Model for Pulsed EM Field Exposure Effects in Cells at Nonthermal Levels

Croce, R. P.; Vita, Assunta De; Pierro, V.; Pinto, I. M.

doi:10.1109/tps.2009.2038163

Cited by 23 publications

(18 citation statements)

References 29 publications

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“…2. Values for the density q M and membrane specific heat c M were chosen to be 10 3 kg/m 3 and 2 9 10 3 J/kg/K, respectively, in keeping with reports [6]. Figure 4 shows the temperature profile.…”

Section: Resultsmentioning

confidence: 99%

Synergistic effects of local temperature enhancements on cellular responses in the context of high-intensity, ultrashort electric pulses

Song

Joshi

Schoenbach

2011

Med Biol Eng Comput

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Results of self-consistent analyses of cells show the possibility of temperature increases at membranes in response to a single nanosecond, high-voltage pulse, at least over small sections of the membrane. Molecular Dynamics simulations indicate that such a temperature increase could facilitate poration, which is one example of a bio-process at the plasma membrane. Our study thus suggests that the use of repetitive high-intensity voltage pulses could open up possibilities for a host of synergistic bio-responses involving both thermal and electrically driven phenomena.

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Section: Resultsmentioning

confidence: 99%

Synergistic effects of local temperature enhancements on cellular responses in the context of high-intensity, ultrashort electric pulses

Song

Joshi

Schoenbach

2011

Med Biol Eng Comput

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“…It is likely that the proteins, such as those embedded in the VG channels, may experience a conformational change and therefore force the gates to open directly due to the ultra-high electric fields. Another potential effect that hasn’t been considered in the MD calculations is the selective heating of the membrane by ultrashort pulses due to a dielectric relaxation process in the plasma membrane, which again might cause faster pore formation at lower membrane voltages [31]. …”

Section: Discussionmentioning

confidence: 99%

A subnanosecond electric pulse exposure system for biological cells

Xiao

Semenov

Petrella

et al. 2016

Med Biol Eng Comput

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An exposure system adapted for use on a microscope stage was constructed for studying the effects of high electric field, subnanosecond pulses on biological cells. The system has a bandpass of 3 GHz and is capable of delivering high voltage electric pulses (6.2 kV) to the electrodes, which are two tungsten rods (100 μm in diameter) in parallel with a gap distance of 170 μm. Electric pulses are delivered to the electrodes through a π-network, which serves as an attenuator as well as an impedance matching unit to absorb the reflection at the electrodes. By minimizing the inductance of the pulse delivery system, it was possible to generate electric fields of up to 200 kV/cm with a pulse duration of 500 ps at the surface of the cover slip under the microscope. The electric field at the cover slip was found to be homogenous over an area of 50 to 70 μm. Within this area, neuroblastoma cells placed on the cover slip were studied with respect to membrane potential changes caused by subnanosecond pulses. This allowed us, for the first time, to demonstrate depolarization of the cell membrane potential.

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“…The majority of the works dealing with this EM approach take into account spherical threelayered geometry to describe extra-cellular medium, plasma membrane, and cytoplasm [2]- [10], [13]. Variable cell radius dimensions from few to tens of μm were analyzed as well as the results of TMP induced by single pulses or trains [9] presenting changeable duration, rise and fall times are presented Only two research groups [11], [12] work on 6th European Conference on Antennas and Propagation (EUCAP) 978-1-4577-0919-7/12/$26.00 ©2011 IEEE developing closed form solution for ellipsoidal prolate and oblate multilayered ellipsoidal geometry subjected to E pulses with variable parameters (e.g. signal durations between 1 ns and few μs).…”

Section: A Analytical Methodsmentioning

confidence: 99%

“…In addition in [13] a chemomechanical model for electroporation-mediated gene delivery is coupled with the EM solution, while in [9] the thermal model of an isolated cell under pulsed electric field is reported.…”

Section: A Analytical Methodsmentioning

confidence: 99%

Microdosimetry for ultrashort electric pulses: A literature review

Merla

Paffi

Apollonio

et al. 2012

2012 6th European Conference on Antennas and Propagation (EUCAP)

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A review of the electromagnetic (EM) approaches used for electric (E) field and transmembrane potential (TMP) evaluation (i.e. microdosimetry) on cells exposed to ultra short-pulsed E fields (usPEF) is presented in this paper. The literature classification is provided on the basis of the EM solution type (i.e. analytical, numerical) and of the analyzed biological target (i.e. single cell, clusters). Moreover, for each EM approach, dielectric modeling of cell compartments is described as well as the various cell shapes employed and pulses characteristics. The EM solutions coupling with biophysical models as those used for membrane electroporation is also discussed. © 2012 IEEE

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A Thermal Model for Pulsed EM Field Exposure Effects in Cells at Nonthermal Levels

Cited by 23 publications

References 29 publications

Synergistic effects of local temperature enhancements on cellular responses in the context of high-intensity, ultrashort electric pulses

Synergistic effects of local temperature enhancements on cellular responses in the context of high-intensity, ultrashort electric pulses

A subnanosecond electric pulse exposure system for biological cells

Microdosimetry for ultrashort electric pulses: A literature review

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