To investigate the fracture properties of the rock-concrete interface after fatigue loading, the fatigue tests with the maximum loads of 60% and 70% of the ultimate load were first carried out under threepoint bending (TPB) loading. After 80000 fatigue loading cycles, the specimens were subjected to quasi-static TPB loading until failure. The fracture parameters including the nominal initial fracture toughness K ini 1C , the nominal unstable fracture toughness K un 1C , the critical crack length ac, the fracture energy Gf and the characteristic length lch were obtained based on the experimental results. The test results indicated that the interfacial crack did not propagate in the fatigue tests if the applied maximum fatigue load was lower than the initial cracking load under quasi-static loading. However, microcracks would occur and accumulate around the pre-crack tip during the fatigue process, which generates a larger damage area than that under the quasi-static load condition. The larger damage area absorbed more energy provided by the applied loading in the subsequent monotonic TPB tests, leading to the increases of the initial cracking load and the nominal initial fracture toughness. Accordingly, a distribution model of the nominal initial fracture toughness along the interfacial ligament was derived for the fatigued specimens. Furthermore, a crack propagation criterion based on the nominal initial fracture toughness was proposed and employed to simulate the fracture process of the fatigued specimens. The good agreements between the experimental and numerical results validated the proposed distribution model and the crack propagation criterion.