A thermoresponsive cationic block copolymer brush-grafted silica bead interface for temperature-modulated separation of adipose-derived stem cells

Thermoresponsive mixed polymer brush to effectively control the adhesion and separation of stem cells by altering temperature

“…The recovered MSCs with adipogenic differentiation exhibit similar PPARγ and LPL expression levels to MSCs without seeding to the PNIPAAm brush and with adipogenic differentiation. We also confirmed the expression of CD90 on MSCs recovered from the PDMAPAAm- b -PNIPAAm brush through flow cytometry [ 68 ]. Thus, the MSCs recovered from the mixed polymer brushes would also have differentiated potency similar to previously reported cell separation materials using PNIPAAm copolymer brushes [ 40 , 68 ].…”

“…We also confirmed the expression of CD90 on MSCs recovered from the PDMAPAAm- b -PNIPAAm brush through flow cytometry [ 68 ]. Thus, the MSCs recovered from the mixed polymer brushes would also have differentiated potency similar to previously reported cell separation materials using PNIPAAm copolymer brushes [ 40 , 68 ].…”

Bioanalytical technologies using temperature-responsive polymers

“…This thermoresponsive polymer has been widely utilized in biomedical applications because of its relatively low LCST, which is close to the human body temperature. Examples of these applications include temperature-controlled drug and gene delivery systems [ 37 – 44 ], biosensors and bioimaging systems [ 45 – 52 ], nanoactutors [ 53 – 59 ], bioseparation tools [ 60 – 74 ], cell separation materials [ 75 – 90 ], and cell culture substrates for tissue engineering [ 91 – 110 ].…”

Temperature-modulated separation of vascular cells using thermoresponsive-anionic block copolymer-modified glass

“…Moreover, PNIPAAm exhibits extension and shrinkage below and above the transition temperature, respectively. The unique properties of PNIPAAm have been applied in various biomedical contexts, such as temperature-regulated drug and gene delivery systems [ [36] , [37] , [38] , [39] , [40] , [41] ], bioanalysis and biosensor devices [ [42] , [43] , [44] , [45] , [46] , [47] ], nano-actuators [ [48] , [49] , [50] , [51] ], bioseparation tools [ [52] , [53] , [54] , [55] , [56] , [57] , [58] , [59] , [60] , [61] , [62] , [63] ], cell-separation materials [ [32] , [33] , [34] , [64] , [65] , [66] , [67] , [68] , [69] , [70] ], cell culture substrates [ [71] , [72] , [73] , [74] , [75] , [76] , [77] ], and cell sheet therapy in diverse types of regenerative medicine [ 5 , [78] , [79] , [80] , [81] , [82] , [83] , [84] , [85] , [86] , [87] , [88] ]. Temperature-controlled cell adhesion and detachment were applied to cell separation procedures using PNIPAAm-modified substrates.…”

“…The incorporation of ionic groups into PNIPAAm is an effective approach for increasing the differences in cell adhesion behavior as cells have different electrostatic properties, which leads to variations in adhesion behavior [ 64 , 65 , 69 , 89 , 90 ]. In addition, smooth muscle cell adhesion is enhanced by the carboxyl group of the PNIPAAm-modified substrate [ 91 ].…”