A third fatty acid Δ9-desaturase from Mortierella alpina with a different substrate specificity to ole1p and ole2p The EMBL accession number for the sequence reported in this paper is AJ278339.

MacKenzie, Donald; Carter, Andrew T.; Wongwathanarat, Prasert; Eagles, John M.; Salt, Joanne; Archer, David B.

doi:10.1099/00221287-148-6-1725

Cited by 37 publications

(13 citation statements)

References 39 publications

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“…D5 desaturase activity on VLCSFAs was demonstrated for ADS proteins from L. alba and A. leveillei (Cahoon et al, 2000;Sayanova et al, 2007). A related desaturase from the fungus Mortierella alpina has also been reported to catalyze synthesis of 26:1 (MacKenzie et al, 2002). Positional diversity of double-bond insertion catalyzed by this class of closely related proteins offers a unique opportunity to explore the mechanisms controlling regiospecificity of membrane-bound fatty acid desaturases.…”

Section: Discussionmentioning

confidence: 99%

Involvement of Arabidopsis ACYL-COENZYME A DESATURASE-LIKE2 (At2g31360) in the Biosynthesis of the Very-Long-Chain Monounsaturated Fatty Acid Components of Membrane Lipids

et al. 2012

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The Arabidopsis (Arabidopsis thaliana) acyl-coenzyme A (CoA) desaturase-like (ADS) gene family contains nine genes encoding fatty acid desaturase-like proteins. The biological function of only one member of the family, fatty acid desaturase5 (AtADS3/ FAD5, At3g15850), is known, and this gene encodes the plastidic palmitoyl-monogalactosyldiacylglycerol D7 desaturase. We cloned seven members of the gene family that are predicted not to have a chloroplast transit peptide and expressed them in the yeast Saccharomyces cerevisiae. All seven have previously undescribed desaturase activity on very-long-chain fatty acid (VLCFA) substrates and exhibit diverse regiospecificity, catalyzing introduction of double bonds relative to the methyl end of the molecule (n-x) at n-6 (AtADS4, At1g06350), n-7 (AtADS1.3, At1g06100 and AtADS4.2, At1g06360), n-9 (AtADS1, At1g06080 and AtADS2, At2g31360) or D9 (relative to the carboxyl end of the molecule) positions (AtADS1.2, At1g06090 and AtADS1.4, At1g06120). Through forward and reverse genetics it was shown that AtADS2 is involved in the synthesis of the 24:1(n-9) and 26:1(n-9) components (X:Y, where X is chain length and Y is number of double bonds) of seed lipids, sphingolipids, and the membrane phospholipids phosphatidylserine, and phosphatidylethanolamine. Plants deficient in AtADS2 expression showed no obvious phenotype when grown under normal growing conditions, but showed an almost complete loss of phosphatidylethanolamine (42:4), phosphatidylserine(42:4), dihydroxy-monohexosylceramide(42:2)-2, trihydroxy-monohexosylceramide(42:2)-3, and trihydroxy-glycosylinositolphosphoceramide(42:2)-3, lipid species that contain the VLCFA 24:1(n-9), and trihydroxyglycosylinositolphosphoceramide(44:2)-3, a lipid containing 26:1(n-9). Acyl-CoA profiling of these plants revealed a major reduction in 24:1-CoA and a small reduction in 26:1-CoA. Overexpression of AtADS2 resulted in a substantial increase in the percentage of glycerolipid and sphingolipids species containing 24:1 and a dramatic increase in the percentage of very-long-chain monounsaturated fatty acids in the acyl-CoA pool. Plants deficient in AtADS1 expression had reduced levels of 26:1(n-9) in seed lipids, but no significant changes in leaf phospholipids or sphingolipids were observed. These findings indicate that the 24-carbon and 26-carbon monounsaturated VLCFAs of Arabidopsis result primarily from VLCFA desaturation, rather than by elongation of long chain monounsaturated fatty acids.

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Section: Discussionmentioning

confidence: 99%

Involvement of Arabidopsis ACYL-COENZYME A DESATURASE-LIKE2 (At2g31360) in the Biosynthesis of the Very-Long-Chain Monounsaturated Fatty Acid Components of Membrane Lipids

et al. 2012

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“…[3][4][5][6][7][8][9][10] These genes have been extensively studied and it was found that addition of UFAs to the growth medium suppressed the expression of OLE1 gene in some yeasts, but not in others. 5,11) Furthermore, in S. cerevisiae, low-temperature stress was found to affect expression of OLE1 gene.…”

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confidence: 99%

Yeast Δ12 Fatty Acid Desaturase: Gene Cloning, Expression, and Function

Watanabe

Oura

Sakai

et al. 2004

Bioscience, Biotechnology, and Biochemistry

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“…Many genes for D9 fatty acid desaturases and D12 fatty acid desaturases have been cloned from fungi and well characterized (Stukey et al 1990;Huang et al 1999;Laoteng et al 1999;Passorn et al 1999;Wongwathanarat et al 1999;Calvo et al 2001;Kajiwara 2002;MacKenzie et al 2002;Watanabe et al 2004), and several of these genes are reported to be induced by low-temperature stress Calvo et al 2001;Nakagawa et al 2002;Watanabe et al 2004). Disruption of the odeA gene of the ascomycetous fungus Aspergillus nidulans, which encodes a D12 fatty acid desaturase, was also shown to influence the development of conidia and ascospores (Calvo et al 2001).…”

Section: Introductionmentioning

confidence: 96%

Cloning and functional characterization of a Δ12 fatty acid desaturase gene from the basidiomycete Lentinula edodes

Sakai

Kajiwara

2005

Mol Genet Genomics

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In the basidiomycete Lentinula edodes, a famous edible mushroom (shiitake), the fatty acyl composition of total lipids has previously been shown to change during cell differentiation. In the present study, we succeeded in cloning a gene for a Delta12 fatty acid desaturase from L. edodes. The ORF of this gene (named Le-FAD2) consists of 1308 bp and codes for 435 amino acids. The deduced Le-FAD2 protein shows 40-45% identity to Delta12 fatty acid desaturases from other fungi, and the three histidine clusters typical of the catalytic domain of such enzymes are conserved. Expression of the Le-FAD2 gene in the budding yeast Saccharomyces cerevisiae indicated that its product was able to synthesize linoleic acid (C18:2). Analysis of Le-FAD2 expression in L. edodes revealed that levels of transcription were higher in fruiting body primordia and in mature fruiting bodies, the two differentiated tissues, than in mycelium, and reduction of the growth temperature from 25 to 18 degrees C had no effect on the level of the Le-FAD2 transcript. Thus, although Le-FAD2 expression is correlated with the alteration in the complement of unsaturated fatty acids (UFAs) observed during fruiting body formation, the gene does not respond to a downshift in temperature to 18 degrees C.

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A third fatty acid Δ9-desaturase from Mortierella alpina with a different substrate specificity to ole1p and ole2p The EMBL accession number for the sequence reported in this paper is AJ278339.

Cited by 37 publications

References 39 publications

Involvement of Arabidopsis ACYL-COENZYME A DESATURASE-LIKE2 (At2g31360) in the Biosynthesis of the Very-Long-Chain Monounsaturated Fatty Acid Components of Membrane Lipids

Involvement of Arabidopsis ACYL-COENZYME A DESATURASE-LIKE2 (At2g31360) in the Biosynthesis of the Very-Long-Chain Monounsaturated Fatty Acid Components of Membrane Lipids

Yeast Δ12 Fatty Acid Desaturase: Gene Cloning, Expression, and Function

Cloning and functional characterization of a Δ12 fatty acid desaturase gene from the basidiomycete Lentinula edodes

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