A third-generation H2O2 biosensor based on horseradish peroxidase-labeled Au nanoparticles self-assembled to hollow porous polymeric nanopheres

Direct electrochemistry of glucose oxidase on screen-printed electrodes through one-step enzyme immobilization process with silica sol–gel/polyvinyl alcohol hybrid film

“…Secondly, they are capable of facilitating the electron transfer between the active sites of enzymes and underlying electrodes. These materials mainly include polymer [5,6], nano-scale materials (carbon nanotubes [7,8], Au nanoparticles [9], metal oxide nanoparticles [10,11]). Generally, a hybrid material needs to be prepared by combining more than one material in order to meet the above-mentioned requirements [12].…”

Expression of CotA laccase in Pichia pastoris and its electrocatalytic sensing application for hydrogen peroxide

“…The performance of the H 2 O 2 biosensor illustrated the potentiality and the advantage of CotA as a transducer for H 2 O 2 detection and preparation of amperometric biosensors. To date, a number of H 2 O 2 biosensors have been reported (ElKaoutit et al 2008;Li et al 2011;Xu et al 2007). Among these reports, horseradish peroxidase was generally used to fabricate the biosensors for the detection of H 2 O 2 .…”

Poly(glycidyl methacrylate‐co‐3‐thienyl‐methylmethacrylate) based working electrodes for hydrogen peroxide biosensing

“…ElKaoutit et al reported a low sensitivity value of 0.133 nA µ(mol L −1 ) −1 although they used a very conductive polymer of nafion and carbon composite. 29 Xu et al found a sensitivity value around 0.15 nA µ(mol L −1 ) −1 using an HRP-labeled gold nanoparticles self-assembled to hollow porous thiol functionalized Poly(divinylbenzene-co-acrylic acid) nanospheres. 28 The Poly(GMA 30% -co-MTM 70% )/PPy/HRP electrode had a lower detection limit (LOD) of 0.06 mmol L −1 , calculated as 3sb/m criteria, 38 where m was the slope of the linear range of the respective calibration plot, and sb was estimated as the standard deviation of the signals from different solutions of H 2 O 2 at the concentration level corresponding to the lowest concentration of the calibration plot.…”