A three-dimensional co-culture model of the aortic valve using magnetic levitation

Tseng, Hubert; Balaoing, Liezl R.; Grigoryan, Bagrat; Raphael, Robert M.; Killian, T. C.; Souza, Glauco R.; Grande-Allen, K. Jane

doi:10.1016/j.actbio.2013.09.003

Cited by 92 publications

(110 citation statements)

References 59 publications

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“…Although the magnetic modification of human cells has become a routine method in tissue engineering research [1,2,10], limited data are currently available on the effects of MNPs on magnetically modified cells. Particularly, magnetization approaches based on intracellular introduction of MNPs into the cytoplasm were used to systematically assess the influence of MNPs on cellular physiology, although to date only limited data has been published, based on either viability dyes tests [27,28] or enzyme activity tests [29].…”

Section: Pah-stabilized Mnps Do Not Affect the Physiological Propertimentioning

confidence: 99%

“…Magnetic functionalization of cells was also employed in fabrication of cellular spheroids [7], which are regarded as three-dimensional building blocks in tissue engineering and a prospective tool in pharmaceutical applications (i.e., drug screening) [8] and tumor studies [9]. MNPs-modified cells were also employed for fabrication of aortic valve mimics using a magnetic levitation procedure [10]. Overall, artificial addition of magnetic functionality in cells allows for effective spatial manipulation using relatively simple and accessible magnetic fields, which dramatically improves control over geometry, size distribution, and cell distribution within multicellular constructs, highlighting magnetic methods in tissue engineering as a promising direction in modern regenerative medicine.…”

Section: Introductionmentioning

confidence: 99%

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Cell surface engineering with polyelectrolyte-stabilized magnetic nanoparticles: A facile approach for fabrication of artificial multicellular tissue-mimicking clusters

et al. 2015

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Regenerative medicine requires new ways to assemble and manipulate cells for fabrication of tissue-like constructs. Here we report a novel approach for cell surface engineering of human cells using polymer-stabilized magnetic nanoparticles (MNPs). Cationic polyelectrolyte-coated MNPs are directly deposited onto cellular membranes, producing a mesoporous semi-permeable layer and rendering cells magnetically responsive. Deposition of MNPs can be completed within minutes, under cell-friendly conditions (room temperature and physiologic media). Microscopy (TEM, SEM, AFM, and enhanced dark-field imaging) revealed the intercalation of nanoparticles into the cellular microvilli network. A detailed viability investigation was performed and suggested that MNPs do not inhibit membrane integrity, enzymatic activity, adhesion, proliferation, or cytoskeleton formation, and do not induce apoptosis in either cancer or primary cells. Finally, magnetically functionalized cells were employed to fabricate viable layered planar (two-cell layers) cell sheets and 3D multicellular spheroids.

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Section: Pah-stabilized Mnps Do Not Affect the Physiological Propertimentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cell surface engineering with polyelectrolyte-stabilized magnetic nanoparticles: A facile approach for fabrication of artificial multicellular tissue-mimicking clusters

et al. 2015

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“…Another successful example is the magnetic nanobeads-mediated spheroid formation [41][42][43][44][45][46][47][48][49] . This method (also known as 'magnetic levitation' when the cells are collected on top of the fluid in a tissue culture well rather than on its bottom 41 ), is extremely versatile.…”

Section: Cell Spheroids As Building Blocks For Bioprintingmentioning

confidence: 99%

Principles of the Kenzan Method for Robotic Cell Spheroid-Based Three-Dimensional Bioprinting

Moldovan

Hibino

Nakayama

2017

Tissue Engineering Part B: Reviews

263

204

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Abstract.Bioprinting is a technology with the prospect to change the way many diseases are treated, by replacing the damaged tissues with live, de novo created bio-similar constructs. However, after more than a decade of incubation and many proofs-of-concept, the field is still in its infancy. The current stagnation is the consequence of its early success: the first bioprinters, and most of those which followed, were modified versions of the 3D printers used in additive manufacturing, redesigned for layer-by-layer dispersion of biomaterials. In all variants (inkjet, micro-extrusion or laser-assisted), this approach is material-('scaffold'-) dependent and energy-intensive, making it hardly compatible with some of the intended biological applications. Instead, the future of bioprinting may benefit from the use of gentler, scaffoldfree bio-assembling methods. A substantial body of evidence has accumulated indicating this is possible by use of preformed cell spheroids, which have been assembled in cartilage, bone and cardiac muscle-like constructs. However, a commercial instrument capable to directly and precisely 'print' spheroids has not been available until the invention of the microneedles-based ('Kenzan') spheroid assembling, and the launching in Japan of a bioprinter based on this method. This robotic platform laces spheroids into predesigned contiguous structures with micron-level precision, using stainless steel micro-needles ("kenzans') as temporary support. These constructs are further cultivated until the spheroids fuse into cellular aggregates and synthesize their own extracellular matrix, thus attaining the needed structural organization and robustness. This novel technology opens wide opportunities for bio-engineering of tissues and organs.

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“…Therefore, the method is said to be universal regarding the phenotype of the involved cells, although the stability of the spheroids and thus of the resulting constructs may vary. Using this regent, a series of high‐profile papers have been published, with examples in vascular,41, 42 pulmonary43 and tumour,44, 45 biology.…”

Section: Recent Developments In Cardiovascular Scaffold‐free 3d Bioprmentioning

confidence: 99%

Progress in scaffold‐free bioprinting for cardiovascular medicine

Moldovan

2018

J Cellular Molecular Medi

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Biofabrication of tissue analogues is aspiring to become a disruptive technology capable to solve standing biomedical problems, from generation of improved tissue models for drug testing to alleviation of the shortage of organs for transplantation. Arguably, the most powerful tool of this revolution is bioprinting, understood as the assembling of cells with biomaterials in three‐dimensional structures. It is less appreciated, however, that bioprinting is not a uniform methodology, but comprises a variety of approaches. These can be broadly classified in two categories, based on the use or not of supporting biomaterials (known as “scaffolds,” usually printable hydrogels also called “bioinks”). Importantly, several limitations of scaffold‐dependent bioprinting can be avoided by the “scaffold‐free” methods. In this overview, we comparatively present these approaches and highlight the rapidly evolving scaffold‐free bioprinting, as applied to cardiovascular tissue engineering.

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A three-dimensional co-culture model of the aortic valve using magnetic levitation

Cited by 92 publications

References 59 publications

Cell surface engineering with polyelectrolyte-stabilized magnetic nanoparticles: A facile approach for fabrication of artificial multicellular tissue-mimicking clusters

Cell surface engineering with polyelectrolyte-stabilized magnetic nanoparticles: A facile approach for fabrication of artificial multicellular tissue-mimicking clusters

Principles of the Kenzan Method for Robotic Cell Spheroid-Based Three-Dimensional Bioprinting

Progress in scaffold‐free bioprinting for cardiovascular medicine

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