A three-dimensional discrete lattice-based system for modeling the growth of aerial hyphae of filamentous fungi on solid surfaces: A tool for investigating micro-scale phenomena in solid-state fermentation

Coradin, Juliana Hey; Braun, Aline; Viccini, Graciele; Luz, Luiz Fernando de Lima; Krieger, Nádia; Mitchell, David A.

doi:10.1016/j.bej.2011.02.012

Cited by 18 publications

(13 citation statements)

References 24 publications

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“…This finding was evidently related to the physical characteristics of the culture medium, with compact and viscous culture medium resulting in difficult mycelial growth. The results obtained through this research were consistent with those attained by other authors [19,30,40].…”

Section: Rhizopus Growth In Different Fluffiness Substrate and Glucoasupporting

confidence: 92%

“…To date, in the study of SSF, ideal technical routes are difficult to obtain by proportional magnification for pure strain culture, biomass within unit volume, and glucoamylase yield. A series of problems, such as poor extending and penetrating capabilities of Rhizopus anoxia mycelia [30] and weak enzyme productivity in the incubator during process scale-up for industrial production, need further research and development. Moreover, the process scale-up for ensuring reactors and hyphae to attain favorable mass and oxygen transfer will become a major research breakthrough for current engineering development [7,31].…”

Section: Introductionmentioning

confidence: 99%

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Application and Analysis of Rhizopus oryzae Mycelia Extending Characteristic in Solid-state Fermentation for Producing Glucoamylase

Tang¹,

Luo²,

Xue³

et al. 2018

Journal of Microbiology and Biotechnology

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Enhanced application of solid-state fermentation (SSF) in industrial production and the influence of SSF of Rhizopus K1 on glucoamylase productivity were analyzed using the flat band method. A growth model was implemented through SSF of Rhizopus K1 in this experiment, and spectrophotometric method was used to determine glucoamylase activity. Results showed that in bran and potato culture medium with 70% moisture in a loose state, µ of mycelium reached to 0.15 h − 1 after 45 h of culture in a thermostatic water bath incubator at 30°C. Under a low-magnification microscope, mycelial cells appeared uniform, bulky with numerous branches, and were not easily ruptured. The generated glucoamylase activity reached to 55 U/g (dry basis). This study has good utilization value for glucoamylase production by Rhizopus in SSF.

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Section: Rhizopus Growth In Different Fluffiness Substrate and Glucoasupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Application and Analysis of Rhizopus oryzae Mycelia Extending Characteristic in Solid-state Fermentation for Producing Glucoamylase

Tang¹,

Luo²,

Xue³

et al. 2018

Journal of Microbiology and Biotechnology

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“…Although our model is relatively simple, it has the flexibility to be extended to describe phenomena that are important in the development of real mycelia. With the incorporation of these modifications, it will be suitable for fusing with the spatial model of Coradin et al [ 25 ] to produce a model that can describe how the development of a fungal mycelium in three dimensions is affected by intracellular transport of nutrients and vesicles. The following sections discuss these points, as well as insights that our modeling work has given into the growth of reproductive aerial hyphae.…”

Section: Discussionmentioning

confidence: 99%

“…Firstly, the model could be used to describe the formation of a branched mycelium, such as that modeled by Coradin et al [ 25 ]. Depending on the fungal species being modeled, the branching would be apical or sub-apical.…”

Section: Discussionmentioning

confidence: 99%

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A Model for Growth of a Single Fungal Hypha Based on Well-Mixed Tanks in Series: Simulation of Nutrient and Vesicle Transport in Aerial Reproductive Hyphae

et al. 2015

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Current models that describe the extension of fungal hyphae and development of a mycelium either do not describe the role of vesicles in hyphal extension or do not correctly describe the experimentally observed profile for distribution of vesicles along the hypha. The present work uses the n-tanks-in-series approach to develop a model for hyphal extension that describes the intracellular transport of nutrient to a sub-apical zone where vesicles are formed and then transported to the tip, where tip extension occurs. The model was calibrated using experimental data from the literature for the extension of reproductive aerial hyphae of three different fungi, and was able to describe different profiles involving acceleration and deceleration of the extension rate. A sensitivity analysis showed that the supply of nutrient to the sub-apical vesicle-producing zone is a key factor influencing the rate of extension of the hypha. Although this model was used to describe the extension of a single reproductive aerial hypha, the use of the n-tanks-in-series approach to representing the hypha means that the model has the flexibility to be extended to describe the growth of other types of hyphae and the branching of hyphae to form a complete mycelium.

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The Cell Factory Aspergillus Enters the Big Data Era: Opportunities and Challenges for Optimising Product Formation

Meyer¹,

Fiedler²,

Nitsche³

et al. 2015

Advances in Biochemical Engineering/Biotechnology

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Living with limits. Getting more from less. Producing commodities and high-value products from renewable resources including waste. What is the driving force and quintessence of bioeconomy outlines the lifestyle and product portfolio of Aspergillus, a saprophytic genus, to which some of the top-performing microbial cell factories belong: Aspergillus niger, Aspergillus oryzae and Aspergillus terreus. What makes them so interesting for exploitation in biotechnology and how can they help us to address key challenges of the twenty-first century? How can these strains become trimmed for better growth on second-generation feedstocks and how can we enlarge their product portfolio by genetic and metabolic engineering to get more from less? On the other hand, what makes it so challenging to deduce biological meaning from the wealth of Aspergillus -omics data? And which hurdles hinder us to model and engineer industrial strains for higher productivity and better rheological performance under industrial cultivation conditions? In this review, we will address these issues by highlighting most recent findings from the Aspergillus research with a focus on fungal growth, physiology, morphology and product formation. Indeed, the last years brought us many surprising insights into model and industrial strains. They clearly told us that similar is not the same: there are different ways to make a hypha, there are more protein secretion routes than anticipated and there are different molecular and physical mechanisms which control polar growth and the development of hyphal networks. We will discuss new conceptual frameworks derived from these insights and the future scientific advances necessary to create value from Aspergillus Big Data.

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A three-dimensional discrete lattice-based system for modeling the growth of aerial hyphae of filamentous fungi on solid surfaces: A tool for investigating micro-scale phenomena in solid-state fermentation

Cited by 18 publications

References 24 publications

Application and Analysis of Rhizopus oryzae Mycelia Extending Characteristic in Solid-state Fermentation for Producing Glucoamylase

Application and Analysis of Rhizopus oryzae Mycelia Extending Characteristic in Solid-state Fermentation for Producing Glucoamylase

A Model for Growth of a Single Fungal Hypha Based on Well-Mixed Tanks in Series: Simulation of Nutrient and Vesicle Transport in Aerial Reproductive Hyphae

The Cell Factory Aspergillus Enters the Big Data Era: Opportunities and Challenges for Optimising Product Formation

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